Separation and Identification of Highly Fluorescent Compounds Derived from trans-Resveratrol in the Leaves of Vitis vinifera Infected by Plasmopara viticola

Abstract

Abstract:A method for identification of highly fluorescent compounds in vine leaves infected by Plasmopara viticola was developed using reversed phase liquid chromatography with simultaneous diode array and fluorometric detection. Fluorescent compounds were extracted from leaves with a methanol-water mixture (70:30). Separation by HPLC was performed using a C 18 column and gradient elution with water-acetonitrile mixtures (20-80% of acetonitrile). The main unknown fluorescent compound was identified by line spectral comparison with a standard obtained by UV photoisomerization of trans-resveratrol glucoside, and its structure was confirmed by liquid chromatography-mass spectrometry. Identification and structural elucidation of the fluorescent compound in the leaves of Vitis vinifera allows early detection of Plasmopara viticola invasion.

Variability in the Content of Trans-Resveratrol, Trans-ε-Viniferin and R2-Viniferin in Grape Cane of Seven Vitis vinifera L. Varieties during a Three-Year Study

“…Data were recorded as mg/kg dry weight. The method was validated in terms of limits of detection and of linearity [ 16 ]. Trans -resveratrol (LOD 0.056 µg/mL, LOQ 0.187 µg/mL), trans -ε-viniferin (LOD 0.158 µg/mL, LOQ 0.525 µg/mL).…”

Various Extraction Methods for Obtaining Stilbenes from Grape Cane of Vitis vinifera L.

“…Also used were an G1315B diode array detector (DAD, Agilent, Prague, Czech Republic) with detection wavelengths at 220 and 315 nm and scanning range 190–600 nm, as well as an G1321A fluorescence detector (FLD, Agilent, Prague, Czech Republic) with excitation wavelength 315 nm, emission wavelength 395 nm, and scanning of emission in the range of 300–600 nm. Finally, the method was validated in terms of linearity, limits of detection, and repeatability [ 17 , 18 ].…”

Trans-pterostilbene and its derivative 2,4-dimethoxy-6-hydroxyphenanthrene in the leaves of Parthenocissus tricuspidata

“…Formation of phenanthrene derivatives as fi nal products in the UV photo isomerization of trans-resveratrol to cis-resveratrol and fi nal cyclization to the derivative phenanthrene in the leaves of Vitis vinifera plants following attack by Plasmopara viticola is described in the literature (Tříska et al 2012). Senescence of Parthenocissus tricuspidata leaves, visibly manifested by the colour change in autumn, may have a similar mechanism also Fig.…”

“…The compounds were identified by measurements made using a LC-MS (LCQ Accela Fleet (Thermo Fisher Scientific, San Jose, CA, USA). Separations using HPLC and LC-MS (APCI) are described in detail in Tříska et al (2012).…”