Separation and Identification of 2,4-Dinitrophenylhydrazones of Aldehydes and Ketones, and 3,5-Dinitrobenzoates of Alcohols by Filter-Paper Chromatography

Abstract

A spectrophotometric method for the estimation of phosphate fractions has been developed which takes advantage of the desirable features of the Fiske and Subbarow method (use of aminonaphtholsulfonic acid as reducing agent) and of the Boltz and Mellon method (development of color by heat in 1 sulfuric acid solution).The advantages of the proposed method are greater sensitivity, greater convenience, and greater specificity over previousmethods.The method is particularly suited for the routine estimation of inorganic phosphate, phosphocreatine, and adenosine triphosphate in biological material (8).

Canning and Storage Effects, Volatile Water-Soluble and Oil Constituents of Valencia Orange Juice

“…The 2,4-dinitrophenylhydrazones were mixtures which could not be resolved by recrvstallization. They were resolved chromatographically (28) and were a mixture of acetone and acetaldehyde 2,4-dinitrophenylhydrazones. The presence of acetone had been demonstrated by Hall and Wilson (70) by means of a potassium nitroprusside test for methyl ketones and an iodoform test.…”

Fixation of carbon dioxide by extracts of the strict autotroph Thiobacillus denitrificans

“…In general sufficient material was applied to the paper to be visible without further treatment. Faint spots were intensified by spraying the paper with 10 % (w/v) aqueous NaOH (Rice, Keller & Kirchner, 1951). Radioactivity.…”

High-performance liquid chromatographic determination of 2-furaldehyde and 5-hydroxymethyl-2-furaldehyde in honey

“…However, both the colorimetric and spectrophotometric methods (i) are time consuming, (ii) make use of toxic or anyhow hazardous chemicals, (iii) require a strict control of both reaction time and temperature, since the instability of the reaction product may lead to low recoveries and wide statistical variations of the results and (iv) none of the methods is specific [3][4][5][7][8][9][10]12,[21][22][23]. Consequently, several chromatographic methods were developed that allow the determination of the individual carbonyls by paper chromatography [24,25], classical liquid-liquid partition chromatography [26], thin-layer chromatography [27], gas chromatography [28][29][30] or high-pressure liquid chromatography (HPLC) [22,31]. These methods are less time consuming, offer improved accuracy, sensitivity and specificity as compared to the colorimetric and spectrophotometric procedures and utilize less hazardous reagents [4,9,10,21].…”