Separation and Fractionation of Aquilaria Malaccensis Oil Using Supercritical Fluid Extraction and tThe Cytotoxic Properties of the Extracted Oil

Ibrahim, Ahmad H.; Al-Rawi, Sawsan S.; Majid, Amin Malik Shah Abdul; Rahman, N.N. Ab.; Salah, K.M. Abo; Kadir, Mohd Omar Ab

doi:10.1016/j.profoo.2011.09.287

Cited by 36 publications

(19 citation statements)

References 9 publications

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“…Another study found that methanolic extract of Aquilaria agallocha woody hull of fruit was effective in inhibiting colorectal cancer cell line [16]. Aquilaria malaccensis stem bark oleoresin extracted via supercritical fluid extraction also exhibited profound cytotoxic effects towards HCT116 human colorectal cancer cell line which further support the finding of this current study [17].…”

Section: Effect Of Abe16 On Cell Attachment and Viabilitysupporting

confidence: 87%

CYTOTOXIC EFFECTS AND RESPONSE SURFACE OPTIMIZATION OF SOLVENT EXTRACTION OF CRUDE EXTRACTS FROM Aquilaria subintegra UNINFECTED BRANCH

Abbas¹,

Hashim²,

Salleh³

2018

Sci. herit. j.

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Agarwood plant has been used in traditional medicine to treat wide range of disorders aside from the main function of its fragrant wood in perfumery. In this present study, we are interested to maximize the yield of extract and investigate the cytotoxic/anti-cancer effects of uninfected branch extracts. Uninfected branch is readily available from agarwood plantation particularly during the early years before inoculation process that formed the fragrant wood (resin); and as such is seen as an economical raw material. Uninfected branch from Aquilaria subintegra was subjected to response surface methodology (RSM)-guided ethanolic extraction to achieve maximum yield while maintaining the biological activity. Cytotoxicity/anti-cancer assays including cell attachment assay, cell viability assay and sulforhodamine B (SRB) assay were carried out on the extracts. Run 11 with 12 hours extraction time, 50 °C temperature, 100 rpm and 60 ml extraction volume gave the highest yield of 0.2130 ± 0.036 g/g agarwood branch extract (ABE). Experimental extract from run 16 showed the most promising cytotoxic effects against MCF-7 with IC50 of 8 µg/ml. The study showed that ABE possess potential cytotoxic/anti-cancer activities against MCF-7 cells and further research is warranted to identify the bioactive compounds and mechanism of action.

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Section: Effect Of Abe16 On Cell Attachment and Viabilitysupporting

confidence: 87%

CYTOTOXIC EFFECTS AND RESPONSE SURFACE OPTIMIZATION OF SOLVENT EXTRACTION OF CRUDE EXTRACTS FROM Aquilaria subintegra UNINFECTED BRANCH

Abbas¹,

Hashim²,

Salleh³

2018

Sci. herit. j.

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“…Mitra, Orbell and Muralitharan (2007) addressed the local ethnomedicinal use of A. malaccensis in treating general dropsy or oedema using A. malaccensis root infusion while its leaves are rubbed over swollen hands and legs of a dropsical patient. This plant has gained enormous interest among researchers due to its antioxidant activity (Huda, Munira, Fitrya, & Salmah, 2009;Miniyar et al, 2008;Nik Wil, Mohd Omar, Awang@Ibrahim, & Tajuddin, 2014;Ray, Leelamanit et al, 2014;Sattayasai et al, 2012;Tay et al, 2014), hepatoprotective effect (Vakati et al, 2013), anticancer activity (Ibrahim et al, 2011), antidiabetic (Zulkifle et al, 2013), and antimicrobial activity (Saad S Dahham et al, 2015;Kamonwannasit et al, 2013). Its essential oil can act as mosquito larvicidal and repellent (Zaridah, Nor Azah, & Rohani, 2006).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of acute and sub-acute oral toxicity of the aqueous extract of Aquilaria malaccensis leaves in Sprague Dawley rats

Razak

Rahman

Hamdan

et al. 2019

APJMBB

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Aquilaria malaccensis or commonly known as ‘gaharu’ is a species of Aquilaria genus and belongs to the Thymelaeaceae family. It is widely distributed in Malaysia, Indonesia, and the Borneo Islands. Traditionally, its leaves were used to relieve bruises and studies have shown that they function as an antioxidant, aphrodisiac, and tranquilizer. Despite its proven beneficial medicinal properties, information regarding its toxicity is limited. Therefore, we performed a safety evaluation on the aqueous A. malaccensis leaves extract (AMAE) in Sprague Dawley rats. The assessment of acute toxicity based on the Organization for Economic Cooperation and Development (OECD) Guideline 420 revealed that AMAE did not influence mortality, clinical appearance, body weight gain, or necropsy findings at a dose of 2000 mg/kg body weight. In the sub-acute toxicity, all doses did not significantly modify the body weight and food and water intake. In male rats treated with 2000 mg/kg, there was a significant reduction in the relative weight of liver. Not only that, an increase in alkaline phosphatase and alanine transaminase was also observed in different groups among the female rats. A significant decrease in the creatinine level was also seen among male rats administered with different doses of AMAE. In both sexes, histopathological analysis had shown abnormalities in the liver and kidney of rats treated at the dose of 2000 mg/kg. In conclusion, the 50% lethal dose (LD50) of AMAE was estimated to be greater than 2000 mg/kg. In sub-acute duration, the findings suggested that AMAE administered orally is slightly toxic at higher doses (2000 mg/kg) and could provoke functional and structural changes in the kidney and liver of rats. Thus, the extract should be used with caution.

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“…However, the cytotoxicity against normal cells was negligible [129]. Another study evaluated the cytotoxicity of the oil fraction of the plant extract against HCT116 colon cancer cells and reported an IC 50 value 4 µg/mL [130]. The major phytoconstituents of the plant were benzaldehyde, pinene, octanol, germacrene, and hexadecanal [131].…”

Section: Thymelaeaceaementioning

confidence: 99%

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

Mazumder

Biswas²,

Raja³

et al. 2020

Molecules

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Cancer or uncontrolled cell proliferation is a major health issue worldwide and is the second leading cause of deaths globally. The high mortality rate and toxicity associated with cancer chemotherapy or radiation therapy have encouraged the investigation of complementary and alternative treatment methods, such as plant-based drugs. Moreover, over 60% of the anti-cancer drugs are molecules derived from plants or their synthetic derivatives. Therefore, in the present review, an attempt has been made to summarize the cytotoxic plants available in the Indian subcontinent along with a description of their bio-active components. The review covers 99 plants of 57 families as well as over 110 isolated bioactive cytotoxic compounds, amongst which at least 20 are new compounds. Among the reported phytoconstituents, artemisinin, lupeol, curcumin, and quercetin are under clinical trials, while brazilin, catechin, ursolic acid, β-sitosterol, and myricetin are under pharmacokinetic development. However, for the remaining compounds, there is little or no information available. Therefore, further investigations are warranted on these subcontinent medicinal plants as an important source of novel cytotoxic agents.

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Separation and Fractionation of Aquilaria Malaccensis Oil Using Supercritical Fluid Extraction and tThe Cytotoxic Properties of the Extracted Oil

Cited by 36 publications

References 9 publications

CYTOTOXIC EFFECTS AND RESPONSE SURFACE OPTIMIZATION OF SOLVENT EXTRACTION OF CRUDE EXTRACTS FROM Aquilaria subintegra UNINFECTED BRANCH

CYTOTOXIC EFFECTS AND RESPONSE SURFACE OPTIMIZATION OF SOLVENT EXTRACTION OF CRUDE EXTRACTS FROM Aquilaria subintegra UNINFECTED BRANCH

Evaluation of acute and sub-acute oral toxicity of the aqueous extract of Aquilaria malaccensis leaves in Sprague Dawley rats

A Review of Cytotoxic Plants of the Indian Subcontinent and a Broad-Spectrum Analysis of Their Bioactive Compounds

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