Separation and determination of chalcones fromCarthamus tinctorius L. and its medicinal preparation by capillary zone electrophoresis

Jiang, Tianyue; Lv, Zhi-Hua; Wang, Yuanhong

doi:10.1002/jssc.200500001

Cited by 20 publications

(12 citation statements)

References 24 publications

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“…To date, ∼ 50 secondary metabolites have been identified in safflower flower extracts; most of them are generalized flavonoids, a class of modulators that mediate bifunctional interactions at vicinal ATP- and steroid-binding sites [9]. These include carthamone, safflor yellow A, hydroxysafflor yellow A, 6-hydroxykaempferol, 6-hydroxykaempferol-3-o-β-D-glucoside, kaempferol [10], [11], [12], and others. The abundance of flavonoids as secondary metabolites makes the safflower a good model for investigating flavonoid biosynthesis in plants, and the related genes and pathways.…”

Section: Introductionmentioning

confidence: 99%

The First Illumina-Based De Novo Transcriptome Sequencing and Analysis of Safflower Flowers

et al. 2012

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BackgroundThe safflower, Carthamus tinctorius L., is a worldwide oil crop, and its flowers, which have a high flavonoid content, are an important medicinal resource against cardiovascular disease in traditional medicine. Because the safflower has a large and complex genome, the development of its genomic resources has been delayed. Second-generation Illumina sequencing is now an efficient route for generating an enormous volume of sequences that can represent a large number of genes and their expression levels.Methodology/Principal FindingsTo investigate the genes and pathways that might control flavonoids and other secondary metabolites in the safflower, we used Illumina sequencing to perform a de novo assembly of the safflower tubular flower tissue transcriptome. We obtained a total of 4.69 Gb in clean nucleotides comprising 52,119,104 clean sequencing reads, 195,320 contigs, and 120,778 unigenes. Based on similarity searches with known proteins, we annotated 70,342 of the unigenes (about 58% of the identified unigenes) with cut-off E-values of 10−5. In total, 21,943 of the safflower unigenes were found to have COG classifications, and BLAST2GO assigned 26,332 of the unigenes to 1,754 GO term annotations. In addition, we assigned 30,203 of the unigenes to 121 KEGG pathways. When we focused on genes identified as contributing to flavonoid biosynthesis and the biosynthesis of unsaturated fatty acids, which are important pathways that control flower and seed quality, respectively, we found that these genes were fairly well conserved in the safflower genome compared to those of other plants.Conclusions/SignificanceOur study provides abundant genomic data for Carthamus tinctorius L. and offers comprehensive sequence resources for studying the safflower. We believe that these transcriptome datasets will serve as an important public information platform to accelerate studies of the safflower genome, and may help us define the mechanisms of flower tissue-specific and secondary metabolism in this non-model plant.

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Section: Introductionmentioning

confidence: 99%

The First Illumina-Based De Novo Transcriptome Sequencing and Analysis of Safflower Flowers

et al. 2012

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“…Hydroxysafflor yellow A is a marker component to control the quality of Carthamus tinctorius L. because of its unambiguous bioactivity 7, 8. Much research on these compounds has been conducted including the separation and isolation,9 quantitative analysis,10 stability,11 and pharmaceutical kinetics and metabolism,12, 13 but no mass spectrometric (MS) characterization has been reported as far as we know. An understanding of their fragmentation rules will make it possible to find more C‐glycosyl quinochalcones without isolation.…”

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confidence: 99%

Characterization of C‐glycosyl quinochalcones in Carthamus tinctorius L. by ultraperformance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Jin

Zhang

Shi

et al. 2008

Rapid Comm Mass Spectrometry

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C-Glycosyl quinochalcones are unique components in Carthamus tinctorius L. The reported C-glycosyl quinochalcones have the same quinochalcone skeleton with a hydroxyl group at the 5'-position and a glucose linked to this position with a carbon-carbon bond. In this study, the standard hydroxysafflor yellow A and water-extracted fraction of Carthamus tinctorius L. were analyzed by ultraperformance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOFMS) in both positive and negative ion modes. The fragmentation pathways of C-glycosyl quinochalcones were interpreted and validated by accurate mass measurement. Their fragmentation showed a special cleavage at the C-C bond except for the typical internal cleavage at the sugar moiety of other C-glycosyl flavonoids. In positive ion mode, cleavage of the 5'-glucose produced an [M+H-162](+) ion by a neutral loss, while cleavage of the 5'-glucose in negative ion mode led to an [M-H-163](-.) ion by radical cleavage. The cleavage from the carbonyl group produced fragment ions containing an A or a B ring. The fragment ions containing an A ring were common product ions of seven compounds in both ion modes, and fragment ions containing the B ring were used to judge the different substituent groups at the 3'-position. The fragmentation patterns of seven structurally related C-glycosyl quinochalcones were analyzed systematically and the formation of the fragment ions in two modes is explained in detail in this report. UPLC/Q-TOFMS is an effective tool for characterizing a complex sample, which gives higher resolution separation and generates accurate mass measurement of the product ions.

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“…Flavonoid glycosides, carthamin, a flavonoid type dye, and safflower yellow are the main constituents in the flower of C. tinctorius [6]. The flowers also contain carthamidin, isocarthamidin, quercetin, kaempferol, 6-hydroxykaempferol and its glycosides, chalcones including hydroxysafflor yellow A, safflor yellow A, safflamin C and safflamin A, and safflomin-A [16][17]. Safflower is useful for the treatment of diabetes and its complications.…”

Section: Resultsmentioning

confidence: 99%

Volatile oil composition of <i>Carthamus Tinctorius</i> L. flowers grown in Kazakhstan

Turgumbayeva

Устенова

Yeskaliyeva

et al. 2018

Ann Agric Environ Med.

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Separation and determination of chalcones fromCarthamus tinctorius L. and its medicinal preparation by capillary zone electrophoresis

Cited by 20 publications

References 24 publications

The First Illumina-Based De Novo Transcriptome Sequencing and Analysis of Safflower Flowers

The First Illumina-Based De Novo Transcriptome Sequencing and Analysis of Safflower Flowers

Characterization of C‐glycosyl quinochalcones in Carthamus tinctorius L. by ultraperformance liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

Volatile oil composition of <i>Carthamus Tinctorius</i> L. flowers grown in Kazakhstan

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