Separation and determination of anthraquinones in Cassia obtusifolia (Leguminosae) by micellar electrokinetic capillary electrophoresis

Abstract

An MEKC method was developed for the determination of the five pharmaceutically important anthraquinones: chrysophanol (1), physcion (2), emodin (3), aloe-emodinin (4), and rhein (5) in Cassia obtusifolia (Leguminosae). A buffer solution (pH 9.00) composed of 20 mM sodium borate, 20 mM sodium deoxycholate (DOC), and 15% ACN was found to be the most suitable electrolyte for this separation. Regression equations revealed linear relationships (correlation coefficients: 0.9993, 0.9992, 0.9996, 0.9989, and 0.9991) between the peak area of each compound (1, 2, 3, 4, and 5) and its concentration. The RSDs of migration times and peak areas were <1.23 and 2.72% within 1 day, respectively. The effects of pH value, surfactant (DOC) concentration, and organic modifier on the migration were also studied. By this way, the contents of five anthraquinones in the extracts of the seed of C. obtusifolia (Leguminosae) from different sources were successfully determined within 14 min.

Simultaneous Determination of Eight Anthraquinones in Semen Cassiae by HPLC‐DAD

“…Several analytical methods determining AQs in SC extracts have been reported. These include UV spectrophotometry, thin‐layer chromatography (TLC), high‐performance liquid chromatography (HPLC) and capillary electrophoresis (CE), with different detectors such as diode array detection (DAD) and mass spectrometry (MS) (Crawford et al , ; Jiang et al , ; Zhang et al , ). However, these methods only allow the quantitation of a few AQ components.…”

Determination of pharmacologically active compounds in root extracts of Cassia alata L. by use of high performance liquid chromatography

“…Several analytical methods such as thin-layer chromatography [28], micellar electrokinetic capillary chromatography [29], capillary zone electrophoresis [30], capillary electrophoresis-mass spectrometry (CE-MS) [31], gas chromatography [32], and gas chromatography-mass spectrometry (GC-MS) [33] have been used for the determination of anthraquinones in various medicinal plants and natural products. In addition, analytical methods involving the use of HPLC [32][33][34][35][36] and liquid chromatographymass spectrometry (LC-MS) [37][38][39][40] have also been used for anthraquinone analysis in several natural products.…”

Discrimination of Semen cassiae from two related species based on the multivariate analysis of high‐performance liquid chromatography fingerprints

“…Semen cassiae, called Juemingzi in Chinese, has been used as a traditional Chinese medicine (TCM) for constipation, asthenic, eye disease and hepatitis, and as a diuretic agent as well as to improve visual acuity . In 2002, Semen cassiae was listed as one of the items that can be used as both food and drug by the Ministry of Health in China.…”