Separation and detection of vitellogenin in fish plasma by capillary zone electrophoresis

Song, Maoyong; Shao, Jing; He, Bin; Jiang, Guibin; Shi, Guoqing

doi:10.1016/j.jchromb.2005.04.013

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Measurement Of Vtg In Zebrafish Blood1

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2007

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“…After centrifugation, serum volumes (2-10 lL) were obtained from individual adult zebrafish, and subsequently diluted in phosphate-buffered saline (PBS) and frozen at À80°C until analysis. Vtg was purified from serum of E2-induced zebrafish according our previous work Song et al, 2005), and used as a standard after identification and quantification. Serum vtg levels were determined using a comparative enzyme-linked immunosorbent assay (ELISA) .…”

Section: Measurement Of Vtg In Zebrafish Bloodmentioning

confidence: 99%