Separation and detection of selenium-containing proteins in human serum

Gao, Yang; Wang, Zijian

doi:10.1007/s002160051599

Cited by 11 publications

(17 citation statements)

References 20 publications

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“…Selenium in the plasma or serum exists as part of albumin (mostly selenomethionine), selenoprotein P (SelP), GPx, and an unknown low molecular weight protein [38]. Only a few studies have examined the different proportions of selenoproteins in plasma or serum [38][39][40][41] and no data is available on the distribution of selenium among serum selenoproteins in breast cancer subjects. In this study, we examined serum selenium and selenoproteins to obtain a more complete understanding of selenium status in breast cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Serum selenium measurements in women with early-stage breast cancer with and without chemotherapy-induced ovarian failure

Breedlove

Smith

Burk

et al. 2006

Breast Cancer Res Treat

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Blood selenium has been shown to decline as breast cancer progresses and fluctuate with estrogen. The objective of this study was to determine the effect of estrogen depletion resulting from chemotherapy-induced ovarian failure on serum selenium and selenoproteins in stage I/II premenopausal breast cancer patients. Serum selenium, glutathione peroxidase (GPx) activity, and selenoprotein P (SelP) were measured and a dietary questionnaire was completed at baseline (before chemotherapy) and 6, 12, and 24 months after start of chemotherapy. Twelve months after the start of adjuvant chemotherapy 33 (75%) patients developed ovarian failure (OF) and 11 (25%) retained menstrual function (non-OF). Dietary selenium intake was 30-58% above the Recommended Dietary Allowance for both groups. By six months the mean estradiol (pg/ml) was lower in the OF group than in the non-OF group (32+/-5 versus 140+/-62 pg/ml, p=0.01) and this difference was maintained at 12 and 24 months. However, there was no differences in serum selenium, GPx activity, or SelP in the OF and non-OF groups at 6, 12, and 24 months. Selenium status in premenopausal breast cancer patients, as measured by serum selenium, GPx and SelP, was within the normal range before and following adjuvant chemotherapy, and was not affected by chemotherapy-induced ovarian failure.

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Section: Discussionmentioning

confidence: 99%

Serum selenium measurements in women with early-stage breast cancer with and without chemotherapy-induced ovarian failure

Breedlove

Smith

Burk

et al. 2006

Breast Cancer Res Treat

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“…In human serum, 4 Se-containing proteins with apparent mol masses of 57-74, 46-56, 40-42, and 21-22 kDa could be separated by gel electrophoresis [30]. The incorporation of Se into protein components was different among the chemical forms applied.…”

Section: Functions and Speciation In Living Organismsmentioning

confidence: 99%

Selenium in agriculture, food, and nutrition

Sager

2006

Pure and Applied Chemistry

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In the case of Se, the concentration range between essentiality and toxicity for terrestric animals and humans is rather narrow, while aquatic organisms are much less affected, and no essentiality to green plants and aquatic macrophytes has been established yet. This review focuses on the situation in Europe, where Se levels are generally low. Apart from industrial and mining activities, the main Se sources are the burning of coal and selenite additions to animal feedstuffs. Reduction processes in sediments, soils, and feedstuffs to yield elemental Se act as sinks for available Se forms. In soils and crops, Se levels get enhanced from recycling of manure, dung, and sewage sludge, which is beneficial for Europe. New data from Austria have been added to the detailed discussions. In human nutrition, Se is supplied via pork, liver and kidneys, seafood, and cereals, but main sources as well as blood Se levels vary between different countries and nutritional habits. Food processing, like boiling, baking, or grilling, results in Se losses.

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“…A detection limit of 0.06 ng Se and quantitative recoveries of Se in glutathione peroxidase were achieved. In a recent study, Se was determined in four protein bands in human serum after SDS-PAGE [37].…”

Section: Atomic Fluorescence Spectrometrymentioning

confidence: 99%

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

McLeod

Tomlinson

et al. 2004

Electrophoresis

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The metabolism of trace elements, in particular their binding to proteins in biological systems is of great importance in biochemical, toxicological, and pharmacological studies. As a result there has been a sustained interest over the last two decades in the speciation of protein-bound metals. Various analytical approaches have been employed, combining efficient separation of metalloproteins by liquid chromatography or electrophoresis with high-sensitivity elemental detection. Slab-gel electrophoresis (GE) is a key platform for high-resolution protein separation, and has been combined with autoradiography and various atomic spectrometric techniques for in-gel determination of protein-bound metals. Recently, the combination of GE with state-of-the-art inductively coupled plasma-mass spectrometry (ICP-MS), particularly when linked to laser ablation (LA) for direct gel interrogation, has opened up new opportunities for rapid characterization of metalloproteins. The use of GE and atomic spectrometry for the speciation of protein-bound trace elements is reviewed in this paper. Technical requirements for gel electrophoresis/atomic spectrometric measurement are considered in terms of method compatibilities, detection capability and potential usefulness. The literature is also surveyed to illustrate current status and future trends.

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Separation and detection of selenium-containing proteins in human serum

Cited by 11 publications

References 20 publications

Serum selenium measurements in women with early-stage breast cancer with and without chemotherapy-induced ovarian failure

Serum selenium measurements in women with early-stage breast cancer with and without chemotherapy-induced ovarian failure

Selenium in agriculture, food, and nutrition

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

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