Separation and detection of oxidation products of fluorodeoxyglucose and glucose by high-performance liquid chromatography–electrospray ionisation mass spectrometry

Buriova, E.; Medová, M; Macášek, F.; Brúder, Peter

doi:10.1016/j.chroma.2004.01.060

Cited by 20 publications

(11 citation statements)

References 25 publications

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“…Preliminary approaches to this analysis carried out by adding ammonium formate52 caused, after a few runs, the deposition of a solid (ammonium or sodium formate) on the transfer line capillary with decrease in sensitivity. On the other hand, when formic acid53 was added to the mobile phase we observed peak broadening effects which decreased the resolution capability of the amino‐bonded column.…”

Section: Resultsmentioning

confidence: 99%

“…Consequently, interferences between oligosaccharides and the matrix components can strongly affect the results by hindering peak identification and integration. With the development of so‐called 'soft' desorption/ionization methods, in particular the electrospray ionization/multi‐stage mass spectrometry (ESI‐MS n ) technique has become more attractive for analysis of underivatized oligosaccharides both in the positive20–46 and negative37, 44, 47–57 ion mode, being sensitive and more selective. For structural elucidation of oligosaccharides, ESI‐MS of positive and negative ions gives complementary information.…”

mentioning

confidence: 99%

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Analysis of underivatized oligosaccharides by liquid chromatography/electrospray ionization tandem mass spectrometry with post‐column addition of formic acid

Verardo

Duse

Callea

2009

Rapid Comm Mass Spectrometry

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Underivatized oligosaccharides were analyzed by electrospray ionization (ESI) using a linear ion trap mass spectrometer in the negative ion mode with post-column addition of an aqueous solution of formic acid. Under these conditions all oligosaccharides showed the presence of the corresponding formate adduct [M + HCOO](-) with high intensity and easy subsequent low-energy collision-induced dissociation (CID) fragmentation using successive MS(n) experiments. A careful examination of the mass spectra obtained from these MS(n) experiments pointed out some significant differences useful to identify and quantify the single components in mixtures of coeluted disaccharides. This new sensitive and rapid method was successfully applied to the quantification of oligosaccharides in some juices minimizing sample handling.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Analysis of underivatized oligosaccharides by liquid chromatography/electrospray ionization tandem mass spectrometry with post‐column addition of formic acid

Verardo

Duse

Callea

2009

Rapid Comm Mass Spectrometry

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“…Figure 4 shows a clear change in zeta potential as the reaction proceeded. The initial drop in zeta potential for the first 15 min is believed to arise from the adsorption of negatively charged dissociated carboxylic anions, formed during oxidation of glucose [21]. This is followed by gradual increase to the original zeta potential as the surface adsorbed intermediates are mineralised.…”

Section: Photocatalytic Mineralisation Of Saccharidesmentioning

confidence: 96%

Photocatalytic mineralisation of organic compounds: a comparison of flame-made TiO2 catalysts

et al. 2007

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A comparative photocatalytic analysis was carried out on TiO 2 made in a Flame Spray Pyrolysis (FSP) process and flame-made Degussa P25. Both have similar crystallinity, phase composition, phase segregation and a non-porous surface. Hence comparison was made based on their difference in specific surface area, organic adsorption and the amount of OH generated upon illumination. The photocatalytic activity tests were carried out using the following series of organic compounds: sucrose, glucose, fructose, maleic acid, glyoxylic acid, oxalic acid, isobutyric acid, phenol and methanol. FSP-made TiO 2 outperformed P25 for saccharides mineralisation, while for phenol and methanol mineralisation P25 was better than FSP-made TiO 2 . Similar mineralisation rates were observed for both FSP-made and P25 TiO 2 for the mineralisation of carboxylic acids. This shows that the relative performance of the photocatalysts depends on the type of organic compounds to be degraded. The high surface area and possibly a more efficient interfacial charge transfer of FSP-made TiO 2 provided an efficient pathway for saccharides mineralisation. As for phenol and methanol, the mineralisation rates were higher when using P25 due to the greater amount of OH radicals generated by this photocatalyst. The fast mineralisation rates of carboxylic acids made degradation of these organic compounds to be less affected by the TiO 2 photocatalyst properties and conditions tested in this work.

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“…Also formed was formate (from both), hydroxyl radicals (from dihydroxyacetone), and ribose (from fructose) (Maksimovic et al, 2006). Furthermore, 60% of 3 mM glucose was oxidized by Fenton radicals at pH 5 in less than 3 h to eight identified products including arabonic and gluconic acids (Buriova et al, 2004). Fe also forms a complex with fructose (Lawrence et al, 2008) and the ferrous iron fructose complex could react with H 2 O 2 to form a more powerful oxidizing agent that oxidizes fructose and especially its carbonyl metabolites.…”

Section: Discussionmentioning

confidence: 99%

Hepatocyte inflammation model for cytotoxicity research: fructose or glycolaldehyde as a source of endogenous toxins

Feng

Wong

Dong³

et al. 2009

Archives of Physiology and Biochemistry

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Insulin resistance and hepatotoxicity induced in high fructose fed rats may involve fructose derived endogenous toxins formed by inflammation. Thus fructose was seventy-fold more toxic if hepatocytes were exposed to non-toxic levels of hydrogen peroxide (H(2)O(2)) released by inflammatory cells. This was prevented by iron (Fe) chelators, hydroxyl radical scavengers, and increased by Fe, copper (Cu) or catalase inhibition. Fructose or glyceraldehyde/dihydroxyacetone metabolites were oxidized by Fenton radicals to glyoxal. Glyoxal (15 microM) cytotoxicity was increased about 200-fold by H(2)O(2). Glycolaldehyde was enzymically formed from glyceraldehyde, the fructokinase/aldolase B product of fructose. Glycolaldehyde cytotoxicity was increased 20-fold by H(2)O(2). The oxidative stress cytotoxicity induced was attributed to the Fenton oxidation of glycolaldehyde forming glycolaldehyde radicals and glyoxal, since cytotoxicity was prevented by aminoguanidine (glyoxal trap) or Fenton inhibitors. Glyoxal was also the Fenton product responsible for glycolaldehyde protein carbonylation as carbonylation was prevented by aminoguanidine or Fenton inhibitors.

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Separation and detection of oxidation products of fluorodeoxyglucose and glucose by high-performance liquid chromatography–electrospray ionisation mass spectrometry

Cited by 20 publications

References 25 publications

Analysis of underivatized oligosaccharides by liquid chromatography/electrospray ionization tandem mass spectrometry with post‐column addition of formic acid

Analysis of underivatized oligosaccharides by liquid chromatography/electrospray ionization tandem mass spectrometry with post‐column addition of formic acid

Photocatalytic mineralisation of organic compounds: a comparison of flame-made TiO2 catalysts

Hepatocyte inflammation model for cytotoxicity research: fructose or glycolaldehyde as a source of endogenous toxins

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