Separation and characterization of the hemoglobin components ofPterygoplichthys pardalis, the acaribodo

Biological aspects of Amazonian fishes. Hemoglobin, hematology, intraerythrocytic phosphates, and whole blood Bohr effect of Mylossoma duriventris

“…Multiple hemoglobins are common in fishes and may be considered an adaptative strategy since, in some cases, they present different functional properties (Brunori et al 1979;Weber et al 1 976;Garlick et al 1 979). For example, there can occur changes in the relative concentrations of hemoglobin fractions as a consequence of environmental variability (Houston and Cyr 1974;Hattingh 1976;Val et al 1985).…”

Structural comparison of the haemoglobin components of the armoured catfish Pterygoplichthys pardalis. Evolutionary considerations

“…These data also show that the estimated sequence differences for haemoglobins from fish belonging to different superorders are of the same order of magnitude; even more important is the fact that the difference between two functionally similar components, i.e. Pt Hb I and trout Hb I (Brunori et al, 1979), is considerably smaller than that observed between functionally distinct haemoglobins belonging to the same animal and actually present within the same erythrocyte (Brun- ori et al, 1974) (compare, in Table 2, trout Hb I with trout Hb IV and Pt Hb I with Pt Hb IV). These observations strongly support the conclusion reached from the comparison of the N-terminal sequences (62 residues) of trout I, trout IV and Catostomus clarkii (a catostomid fish) a-chains (Bossa et al, 1978).…”

“…Blood obtained from several speciments of Pterygoplichthys pardalis was collected during the Alpha-Helix expedition in the Amazonas (November-December, 1976) and the haemolysate was kept as an (NH4)2SO4 precipitate under pure CO at -200C. Separation of the four haemoglobin components was achieved as described by Brunori et al (1979).…”

“…For these reasons, during the 'Alpha-Helix' expedition in the Amazonas, the four haemoglobin components of Pterygoplichthys were separated and characterized from the functional point of view (Brunori et al, 1979). It was shown that these haemoglobins can be divided in two groups characterized by distinct and complementary functional properties: one group is represented by the most abundant haemoglobin, component I, the other comprises components II, III and IV.…”

Estudos de hemoglobinas em peixes da Amazônia : uma revisão

“…A grande multiplicidade e o polimorfísmo de hemoglobinas em vertebrados estão da mesma maneira, bem esta belecidos. Os estudos de hemolisados de peixes da Amazônia (Fyhn et a 1»,1979) e freqüente e que a poliploidia é amplamente distribuída (Ohno, 1970;Schrõder ed 1973 Brunori et al , 1979;Garlick et al, í979b). Bonaventura et al (1975) (Watt & Riggs, 1975), Weber et al, (1976c) sugerem que hemoglobinas insensíveis a mudanças de temperatura podem estabilizar sua afinidade ao oxigênio durante a variação deste parâmetro.…”