Separation and Characterization of Intestinal Brush Border
Enzymes in Adult Rats and in Suckling Rats under Normal
Conditions and after Hydrocortisone Injections

Raul, F.; Pm, Simon; Kédinger, Michèle; Jf, Grenier; Haffen, K

doi:10.1159/000458557

Cited by 34 publications

(17 citation statements)

References 19 publications

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“…The proximal part of the small intestine was resected, everted and the mucosa was removed. Partial purification of brush border membranes has been performed by a modification (14) of the procedure of Schmitz et al (18). The brush border proteins were separated by gel electrophoresis on 7.5% polyacrylamide gels containing 0.1% sodium dodecyl sulfate (8).…”

Section: Methodsmentioning

confidence: 99%

“…Enzymes were eluted from the slices by overnight incubation of each slice in 1 ml of distilled water at 4 °C. Disaccharidases [isomaltase (substrate:palatinose), lactase, maltase, sucrase) as well as glucoamylase were determined according to Messer and Dahlqvist (12) on aliquots of the gel eluates (14) and of the starting homogenates (lactase, sucrase). The enzyme activities of the ho mogenates were expressed as mU/mg protein (mean t SEM).…”

Section: Methodsmentioning

confidence: 99%

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Sucrase and Lactase Synthesis in Suckling Rat Intestine in Response to Substrate Administration

et al. 1978

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The intestinal brush border disaccharidases separated by gel electrophoresis were studied after oral administration of a high sucrose or lactose diet to 11-day-old suckling rats during 3 days. Some modifications of the brush border protein and enzyme patterns could be attributed to the effect of the basic diet: increase of glucoamylase, appearance of a weak sucrase activity and of a second molecular form of maltase. However, the specific action of a given disaccharide on the synthesis of the corresponding hydrolytic enzyme could be clearly demonstrated. Indeed, the electrophoretic pattern after sucrose or lactose feeding showed a marked increase of the protein bands corresponding to sucrase-isomaltase or lactase activities.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Sucrase and Lactase Synthesis in Suckling Rat Intestine in Response to Substrate Administration

et al. 1978

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“…2 mM Tris, pH 7. Partial purifica tion of brush border membranes was performed as described earlier [22] on intestinal homogenates. En zyme activities were measured in the purified brush border fraction.…”

Section: Determination O F Proteins and O F Enzyme Activitiesmentioning

confidence: 99%

Comparative in vivo and in vitro Effect of Mono- and Disaccharides on Intestinal Brush Border Enzyme Activities in Suckling Rats

Raul¹,

Kédinger²,

Simon³

et al. 1981

Neonatology

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Suckling rats were bottle fed during 48 h with a basic diet enriched with different mono- or disaccharides. In parallel, explants of intestinal mucosa were cultured in vitro for 48 h in the presence or in the absence of a synthetic glucocorticoid (dexamethasone) and with or without different carbohydrates. In both studies, enzyme activities were assayed on purified brush border membranes. From these combined in vivo and in vitro investigations it appeared that (1) there is a highly specific stimulation of mono- and disaccharides on the corresponding brush border disaccharidases: glucose, fructose, sucrose on sucrase and maltase activities, fructose being generally the most potent activator. Other brush border enzymes were not modified by the dietary carbohydrates. (2) These sugar-mediated effects were obtained only in the presence of glucocorticoids. This hormone alone induced the appearance of a slight sucrase activity and provoked a stimulation of maltase activity. The results show clearly that glucocorticoids are necessary to induce sucrase activity, but that the level of this activity is under the strict dependence of the dietary carbohydrates.

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“…It is generally assumed that it is the glucocorticoid hormone which triggers the maturation. The serum levels of glucocorticoid hormones increase rapidly at the time of weaning (2) and treatment with large doses of glucocorticoid in suckling rats results in precocious development of enzymes in the gastrointestinal tract (3)(4)(5)(6).…”

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Role of Aldosterone for Control of Colonic NaKATPase Activity in Weanling Rats

Finkel

Aperia

1986

Pediatr Res

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ABSTRACT. Ex~eriments were ~erforrned to examine MATERIALS AND METHODShow aldosterone ;noddates coloniE NaKATPase activity during the weaning period. NaKATPase activity was determined in proximal and distal colon in control rats aged 16, 20, and 40 days, in rats aged 20 and 40 days fed low sodium diet for 4 days and in 20-day-old rats adrenalectomized on day 16. In some protocols net sodium and water transport was determined with in vivo perfusion technique. In control rats colonic NaKATPase activity increased significantly between day 16 and 20. This increase was abolished by adrenalectomy but restored by aldosterone substitution, 5 pg/lOO g body weight112 h. No significant increase in NaKATPase activity occurred between day 20 and 40. Serum levels of both aldosterone and of corticosterone were low until day 14 and increased to peak level at day 18-20. In 20-and 40-day-old rats fed a low sodium diet, NaKATPase activity increased significantly in proximal and distal colon in both age groups but the increases were significantly greater in the 20-than the 40-day-old animals. A low sodium diet increased serum aldosterone, but not serum corticosterone levels in both age groups: also the low sodium diet significantly increased net sodium and water transport in 20-but not in 40-day-old rats. Aldosterone is of physiological importance for the regulation of Pi, inorganic phosphorousAt the time of weaning there is an accelerated maturation of gastrointestinal enzymes and this maturation is attenuated by adrenalectomy (1). It is generally assumed that it is the glucocorticoid hormone which triggers the maturation. The serum levels of glucocorticoid hormones increase rapidly at the time of weaning (2) and treatment with large doses of glucocorticoid in suckling rats results in precocious development of enzymes in the gastrointestinal tract (3-6).There are few developmental studies of the large intestine. The adult large intestine has a high concentration of mineralocorticoid receptors (7,8), and the activity of NaKATPase enzyme in the adult colon can be modulated by mineralocorticoid (9). The present study was designed to explore the influence of mineralocorticoid hormone on colonic NaKATPase activity at the time of weaning. Animals. Sprague-Dawley rats from Anticimex, Sollentuna, Sweden were used. The animal quarters were air-conditioned (2 1 + lo C) with a 12-h light cycle (0700-1900 h) and a 12-h dark cycle. The litter sizes were maintained at four to six animals.Experimental Groups. Zn~act rats. The animals were kept with lactating dams. They had free access to ordinary rat food (Ewos R3) containing 0.13 mmol Na/g and tap water ad libitum until 20 days. Thereafter they were kept in litters without dams. Their body weight increased from a mean of 21.5 g at 10 days to a mean of 170 g at 40 days. Sodium depletion. These animals were fed a low sodium diet (Ewos) for 4 days. The diet was similar to the ordinary rat food but contained 0.009 mmol Na/g. Animals aged 16 days were separated from the dams and fed a low salt diet...

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Separation and Characterization of Intestinal Brush Border Enzymes in Adult Rats and in Suckling Rats under Normal Conditions and after Hydrocortisone Injections

Cited by 34 publications

References 19 publications

Sucrase and Lactase Synthesis in Suckling Rat Intestine in Response to Substrate Administration

Sucrase and Lactase Synthesis in Suckling Rat Intestine in Response to Substrate Administration

Comparative in vivo and in vitro Effect of Mono- and Disaccharides on Intestinal Brush Border Enzyme Activities in Suckling Rats

Role of Aldosterone for Control of Colonic NaKATPase Activity in Weanling Rats

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