Separation and characterization of benzaldehyde-functional polyethylene glycols by liquid chromatography under critical conditions

Abstract

Baseline separation and characterization of benzaldehyde-substituted PEGs based on end-group functionality achieved by LCCC and confirmed by off-line MALDI-TOF-MS.

Reverse-phase high performance liquid chromatography (RP-HPLC) as a powerful tool to characterise complex water-soluble copolymer architectures

“…[37][38][39] At this critical point, namely a set of temperature and solvent conditions at which enthalpic and entropic factors are balanced for one polymer constituent, retention time is independent of molar mass of one of the homopolymer and therefore the retention time for copolymers is only reliant of chain length of the other block component. 40,41 This was successfully used to characterise a large number of water-soluble [42][43][44] and water-insoluble copolymers, 45,46 but requires time-consuming optimisation to determine the critical point of the studied system. Alternatively, two-dimensional approaches in which liquid chromatography techniques are coupled to another characterisation device such as gel permeation chromatography (GPC) 47 or mass spectrometry (MS) 48,49 have also been used in the past to generate rich maps of copolymers structure, yet these requires complex equipment which might not be accessible to most laboratories.…”

Characterization of N-phenylmaleimide-terminated poly(ethylene glycol)s and their application to a tetra-arm poly(ethylene glycol) gel

“…Modified and unmodified PEGs can be distinguished by isocratic elution using a reversed-phase column (C18) via the changes in their polarity when functional groups are attached to the ends of the polymer chains. 49,50 In this paper, we report a novel characterization method to evaluate the conversion and purity of N-aryl MA-bearing PEGs using HPLC. The establishment of this characterization method also provides a guideline for N-aryl MA-functionalized PEGs, because the ''PEGylation'' of drugs, peptides, proteins, and PEG-based micelles has been widely used in various fields.…”