Separation and Biological Activities of Phospholipase A2 (Mb-PLA2) from the Venom of <i>Montivipera bornmuelleri</i>, a Lebanese Viper

Accary, Claudine; Mantash, Aziza; Mallem, Yassine; Fajloun, Ziad; Elkak, Assem

doi:10.1080/10826076.2014.968660

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…For example, it was reported that snake venom (Bernardes-Oliveira et al, 2016), and more precisely Phospholipases A2 (Prinholato da Silva et al, 2015), can lead to cell cycle arrest at the G0/G1 phase. M. bornumelleri's venom is known to contain Phospholipases A2 (Accary et al, 2015); therefore, it will not be surprising if the venom affects the cell cycle in a similar way to what was reported previously (Bernardes-Oliveira et al, 2016;Prinholato da Silva et al, 2015).…”

Section: Discussionsupporting

confidence: 73%

Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies

Haddoub

Rima

Heurtebise

et al. 2020

PeerJ

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Background Montivipera bornmuelleri’s venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating the sensitivity of murine B16 skin melanoma and 3-MCA-induced murine fibrosarcoma cell lines to M. bornmuelleri’s venom and its effect on tumor growth in vivo. Methods The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. Results The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 µg/kg. Our study demonstrates a cytotoxic effect of M. bornmuelleri’s venom in vitro which, however, does not translate to an anticancer action in vivo.

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Section: Discussionsupporting

confidence: 73%

Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies

Haddoub

Rima

Heurtebise

et al. 2020

PeerJ

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“…In the literature, it has been shown that snake venom components can induce pro-inflammatory cytokines secretion and mediate tissue damage. In accordance, PLA2 isolated from the venom of M. bornmuelleri exhibited pro-inflammatory activities in vitro [ 13 ]. This could be explained by the fact that this venom component can act directly on leukocytes, macrophages and endothelial cells to stimulate cytokine production.…”

Section: Discussionmentioning

confidence: 95%

“…Many studies focused on identifying, characterizing, and purifying proteins within M. bornmuelleri crude venom, while other studies highlighted the biological properties of the venom in vitro [ [11] , [12] , [13] , [14] ]. However, the in vivo effects of this Lebanese snake venom is poorly known.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, it was suggested that M. bornmuelleri venom lacks direct lytic factors since it induces indirect hemolysis in the presence of PLA2, which is able to hydrolyze lecithin [ 11 ]. To further characterize the role of PLA2, it was purified and its biological activity was tested showing that it exhibits strong anti-bacterial, hemolytic, anti-coagulant and pro-inflammatory activities [ [13] , [14] ].…”

Section: Introductionmentioning

confidence: 99%

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Montivipera bornmuelleri venom has immunomodulatory effects mainly up-regulating pro-inflammatory cytokines in the spleens of mice

et al. 2018

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“…To further characterize the role of PLA2 in this Lebanese viper venom, the purified enzyme was tested for its biological activity. It has been shown that M. bornmuelleri’s PLA2 exhibits strong anti-bacterial, hemolytic, anti-coagulant, and pro-inflammatory activities [ 59 ]. This is in accordance with previous studies elucidating the effects of the crude venom of M. bornmuelleri as well as studies revealing the varied properties of PLA2.…”

Section: Montivipera Bornmuellerimentioning

confidence: 99%

Vipers of the Middle East: A Rich Source of Bioactive Molecules

et al. 2018

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Snake venom serves as a tool of defense against threat and helps in prey digestion. It consists of a mixture of enzymes, such as phospholipase A2, metalloproteases, and l-amino acid oxidase, and toxins, including neurotoxins and cytotoxins. Beside their toxicity, venom components possess many pharmacological effects and have been used to design drugs and as biomarkers of diseases. Viperidae is one family of venomous snakes that is found nearly worldwide. However, three main vipers exist in the Middle Eastern region: Montivipera bornmuelleri, Macrovipera lebetina, and Vipera (Daboia) palaestinae. The venoms of these vipers have been the subject of many studies and are considered as a promising source of bioactive molecules. In this review, we present an overview of these three vipers, with a special focus on their venom composition as well as their biological activities, and we discuss further frameworks for the exploration of each venom.

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Separation and Biological Activities of Phospholipase A2 (Mb-PLA2) from the Venom of Montivipera bornmuelleri, a Lebanese Viper

Cited by 12 publications

References 24 publications

Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies

Cytotoxic effect of Montivipera bornmuelleri’s venom on cancer cell lines: in vitro and in vivo studies

Montivipera bornmuelleri venom has immunomodulatory effects mainly up-regulating pro-inflammatory cytokines in the spleens of mice

Vipers of the Middle East: A Rich Source of Bioactive Molecules

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