Separation and assay methods for melatonin and its precursors

Harumi, Tatsuo; Matsushima, Shoji

doi:10.1016/s0378-4347(00)00064-5

Cited by 44 publications

(26 citation statements)

References 60 publications

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“…The identification of endogenous IAA and MEL was made by MS/ESI+. Selected ion monitoring (SIM) in the multiple reaction monitoring (MRM) of the daughter ions, in the case of IAA (m/z 176 fi 130) and in the case of MEL (m/z 233 fi 174), in lupin samples unequivocally confirmed their identification (Harumi and Matsushima 2000;Yang et al 2002). Table 1 shows the IAA and MEL levels in different hypocotyl zones: apical, central and basal.…”

Section: Resultsmentioning

confidence: 71%

Melatonin: a growth-stimulating compound present in lupin tissues

Hernández‐Ruíz

Caño

Arnao

2004

Planta

306

218

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Melatonin (N-acetyl-5-methoxi-tryptamine), a well-known animal hormone synthetised by the pineal gland, plays a key role in the circadian rhythm of vertebrates. An exhaustive bibliographical revision of studies on melatonin in plants published since 1990 points to very few studies (around 20), of which only 8 have a clear plant physiological focus. The data presented in this study demonstrate that melatonin plays a physiological role in plant tissues. Melatonin is seen to be a molecule that promotes vegetative growth in etiolated Lupinus albus L. hypocotyls, in a similar way to IAA. The measurements of melatonin and IAA in lupin hypocotyls by high-performance liquid chromatography with electrochemical detection, and their identification by tandem mass spectrometry, point to a different distribution of these molecules in etiolated hypocotyls.

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Section: Resultsmentioning

confidence: 71%

Melatonin: a growth-stimulating compound present in lupin tissues

Hernández‐Ruíz

Caño

Arnao

2004

Planta

306

218

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“…Several analytical techniques have been developed for the simultaneous measurement of 5-HT and MT, liquid chromatography coupled to mass spectrometry and capillary electrophoresis with UV detection being most often used [16][17][18][19]. Although these methods can adequately monitor the levels of 5-HT and MT, they still require skilled personnel and sophisticated and expensive instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Screen-printed electrodes modified with carbon nanotubes or graphene for simultaneous determination of melatonin and serotonin

et al. 2015

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Single-walled carbon nanotubes (SWCNT), multiwalled carbon nanotubes (MWCNT) and graphene have been tested as carbon allotropes for the modification of carbon screen-printed electrodes (CSPEs) to simultaneously determine melatonin (MT) and serotonin (5-HT). Two groups of CSPEs, both 4 mm in diameter, were explored: The first includes commercial SWCNT, MWCNT and graphene, the second includes SWCNT, MWCNT, graphene oxide nanoribbons and reduced nanoribbons that were drop casted on the electrodes. The carbon nanomaterials enhanced the electroactive area in the following order: CSPE

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“…1 Since its structure was first discovered in 1958 by Lerner, 2 many studies about melatonin in the scientific literature were published, making melatonin one of the hottest subjects of life extension research. 3,4 Many analytical methods have been developed for the determination of melatonin and its related indoleamines, [5][6][7][8] in which, chemiluminescence (CL) methods have also been used for certain indole derivatives. [9][10][11][12][13] For example, Alwarthan 11 determined tryptophan using flow injection CL system based on the oxidation of tryptophan with cerium(IV) in sulfuric acid.…”

Section: Introductionmentioning

confidence: 99%

Determination of Melatonin in Rat Pineal Gland and Drug with Flow‐Injection Chemiluminescence

et al. 2007

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A flow-injection chemiluminescence (CL) method for the determination of melatonin based on the CL reaction of melatonin with hydrogen peroxide and sodium hypochlorite (NaOCl) in a basic alkaline solution was developed. The possible CL mechanism has been discussed, and a proposal for the reaction pathway was given that singlet oxygen was clarified to be produced in this reaction system and was responsible for the CL emission. Under the optimized conditions, the linear concentration range of application was 1.0×10 -7 -2.5×10 -4 mol•L -1 with a detection limit of 5.0×10 -8 mol•L -1 (S/N＝3). The relative standard deviation for eight repeated measurements of 1.0 ×10 -6 mol•L -1 melatonin was 2.8%. The interferences of several important biological substances, some indole compound, cations and anions were studied. No interference was found for the anions, glucose, starch, most of cations and low concentration (less than 3.0×10 -6 mol•L -1 ) of some biological substances and indole compound. The method was applied to the determination of melatonin in rat pineal gland and drug with satisfactory results. The sample throughput was 90 injections per hour.

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Separation and assay methods for melatonin and its precursors

Cited by 44 publications

References 60 publications

Melatonin: a growth-stimulating compound present in lupin tissues

Melatonin: a growth-stimulating compound present in lupin tissues

Screen-printed electrodes modified with carbon nanotubes or graphene for simultaneous determination of melatonin and serotonin

Determination of Melatonin in Rat Pineal Gland and Drug with Flow‐Injection Chemiluminescence

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