Separation and Analysis of Peptides and Proteins

Abstract

This review focuses on selected applications of the separation and analysis of peptides and proteins published during the period of 1997-1998. Specific topic areas covered include high-performance liquid chromatography (HPLC), ultrafiltration, capillary electrophoresis (CE), affinity-based methods for protein isolation and separation, mass spectrometry (MS), detection of nonenzymatic posttranslational modifications, nuclear magnetic resonance spectroscopy (NMR), infrared (IR) and Raman spectroscopy, circular dichroism (CD), UV-visible absorption spectroscopy, dynamic light scattering, and calorimetry. The quantification and identification of peptides and proteins by chromatographic methods and MS have become fairly routine, as has the conformational analysis of peptides and small proteins in solution by CD, IR, and NMR. Therefore, these topics are not reviewed in detail here. In this review, we have attempted to highlight new technological developments or unique applications of analytical methods that impact the analysis of peptides and proteins.

Separation of diastereomers of phosphinic pseudopeptides by capillary zone electrophoresis and reverse phase high‐performance liquid chromatography

“…HPLC is a well-established technique for peptide analysis [17,18] and it is currently also used, mostly in reverse phase mode (RP-HPLC), for routine separations of stereoisomers in crude synthetic products [19,20]. A separation of diastereomers of synthetic analogues of deltorphin C and enkephalin by PØter et al [21], a study on a series of D-isomer substitutions of 36-peptide neuropeptide Y by Kirby et al [22], an ion-pair RP-HPLC separation of diastereomers of tripeptide thymotrinan and tetrapeptide thymocartin by Gazdag et al [23], or a separation of tryptophan peptide diastereomers by Griehl et al [24] may serve as relevant examples of successful RP-HPLC separations of peptide diastereomers.…”

Chitosan Based Membranes for Separation, Pervaporation and Fuel Cell Applications: Recent Developments

“…Adsorption performances of the composite membranes for Cu 2+ ions and BSA on the surface were compared with CA hollow fibers membranes. The blend hollow fibers membranes achieved significantly better adsorption performance as compared to CA hollow fibers, indicating the benefit of adding CS into CA to make novel blend hollow fibers membranes in improving the performance of the traditional CA hollow fibers, especially for the affinity-based separations [70]. Compared to chromatographic methods, membrane separation techniques offer advantages of lower cost and ease to scale-up for commercial production.…”

Recent Developments in Capillary Electrophoresis–Mass Spectrometry of Proteins and Peptides

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14] By building on these and earlier efforts, it is anticipated that the popularity of CE-MS will grow, and its advantages will be better appreciated and exploited.…”