Separating large microscale particles by exploiting charge differences with dielectrophoresis

Polniak, Danielle V.; Goodrich, Eric; Hill, Nicole; Lapizco‐Encinas, Blanca H.

doi:10.1016/j.chroma.2018.02.051

Cited by 25 publications

(16 citation statements)

References 61 publications

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“…As shown in Fig. A, the post array spans around 3 cm, which is much longer than traditional iDEP devices used by our group. Particle samples were introduced electrokinetically into the channel and allowed to migrate through the post array by applying a DC potential between the channel inlet and outlet.…”

Section: Streaming Idep Systemsmentioning

confidence: 95%

On the use of correction factors for the mathematical modeling of insulator based dielectrophoretic devices

Hill

Lapizco‐Encinas

2019

Electrophoresis

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Mathematical modeling is a fundamental component in the development of new microfluidics techniques and devices. Modeling allows for the rapid testing of new system configurations while saving resources. Microscale electrokinetic (EK) techniques have significantly benefited by the advances in modeling programs and software packages. However, EK phenomena are complex to model, as they dynamically affect system characteristics, including the physical properties of the particles and fluid within the system. Insulator‐based dielectrophoresis (iDEP) is an EK technique that has received important attention during the last two decades. In particular, numerous research groups that study iDEP systems employ a combination of modeling and experimentation for developing new iDEP systems. An important fraction of these research groups has adopted the practice of employing “correction factors” to account for EK phenomena that cannot be accurately predicted in their models due to model complexity and limitations in computing resources. The present review article aims to provide the reader with an overview of the most common approaches in the use of correction factors for the modeling of iDEP systems.

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Section: Streaming Idep Systemsmentioning

confidence: 95%

On the use of correction factors for the mathematical modeling of insulator based dielectrophoretic devices

Hill

Lapizco‐Encinas

2019

Electrophoresis

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“…The time for the cells to revert to the original pattern of distribution depended on the kind of polarisation and cell size. The larger cell size had a slower change compared to the smaller cells [54].…”

Section: Discussionmentioning

confidence: 99%

Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre

et al. 2019

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This paper describes the development of an integrated system using a dry film resistant (DFR) microfluidic channel consisting of pulsed field dielectrophoretic field-flow-fractionation (DEP-FFF) separation and optical detection. The prototype chip employs the pulse DEP-FFF concept to separate the cells (Escherichia coli and Saccharomyces cerevisiae) from a continuous flow, and the rate of release of the cells was measured. The separation experiments were conducted by changing the pulsing time over a pulsing time range of 2–24 s and a flow rate range of 1.2–9.6 μ L min − 1 . The frequency and voltage were set to a constant value of 1 M Hz and 14 V pk-pk, respectively. After cell sorting, the particles pass the optical fibre, and the incident light is scattered (or absorbed), thus, reducing the intensity of the transmitted light. The change in light level is measured by a spectrophotometer and recorded as an absorbance spectrum. The results revealed that, generally, the flow rate and pulsing time influenced the separation of E. coli and S. cerevisiae. It was found that E. coli had the highest rate of release, followed by S. cerevisiae. In this investigation, the developed integrated chip-in-a lab has enabled two microorganisms of different cell dielectric properties and particle size to be separated and subsequently detected using unique optical properties. Optimum separation between these two microorganisms could be obtained using a longer pulsing time of 12 s and a faster flow rate of 9.6 μ L min − 1 at a constant frequency, voltage, and a low conductivity.

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“…For microfluidic technology, it is fundamental to pump and mix fluids, electrokinetic offers an option to avoid mechanical pumps with electro-osmosis [ 60 ]. Additionally, separation of phases or heterogeneous solutions can be done with electrophoresis and dielectrophoresis [ 61 , 62 ]. A principles representation of the main electrokinetic phenomena is presented in Figure 3 .…”

Section: Electrokinetic Phenomena: Theory and Microfluidic Applicamentioning

confidence: 99%

Organs-on-a-Chip Module: A Review from the Development and Applications Perspective

et al. 2018

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In recent years, ever-increasing scientific knowledge and modern high-tech advancements in micro- and nano-scales fabrication technologies have impacted significantly on various scientific fields. A micro-level approach so-called “microfluidic technology” has rapidly evolved as a powerful tool for numerous applications with special reference to bioengineering and biomedical engineering research. Therefore, a transformative effect has been felt, for instance, in biological sample handling, analyte sensing cell-based assay, tissue engineering, molecular diagnostics, and drug screening, etc. Besides such huge multi-functional potentialities, microfluidic technology also offers the opportunity to mimic different organs to address the complexity of animal-based testing models effectively. The combination of fluid physics along with three-dimensional (3-D) cell compartmentalization has sustained popularity as organ-on-a-chip. In this context, simple humanoid model systems which are important for a wide range of research fields rely on the development of a microfluidic system. The basic idea is to provide an artificial testing subject that resembles the human body in every aspect. For instance, drug testing in the pharma industry is crucial to assure proper function. Development of microfluidic-based technology bridges the gap between in vitro and in vivo models offering new approaches to research in medicine, biology, and pharmacology, among others. This is also because microfluidic-based 3-D niche has enormous potential to accommodate cells/tissues to create a physiologically relevant environment, thus, bridge/fill in the gap between extensively studied animal models and human-based clinical trials. This review highlights principles, fabrication techniques, and recent progress of organs-on-chip research. Herein, we also point out some opportunities for microfluidic technology in the future research which is still infancy to accurately design, address and mimic the in vivo niche.

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Separating large microscale particles by exploiting charge differences with dielectrophoresis

Cited by 25 publications

References 61 publications

On the use of correction factors for the mathematical modeling of insulator based dielectrophoretic devices

On the use of correction factors for the mathematical modeling of insulator based dielectrophoretic devices

Separation and Detection of Escherichia coli and Saccharomyces cerevisiae Using a Microfluidic Device Integrated with an Optical Fibre

Organs-on-a-Chip Module: A Review from the Development and Applications Perspective

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