Separate regulatory elements for the heat-inducible and ovarian expression of the drosophila hsp26 gene

Abstract

We have constructed and transformed into the D. melanogaster germ line a series of hsp26 deletion variants. We show that the region -728 to +14 contains all of the cis-acting sequences necessary for the developmental and heat-inducible transcription of hsp26. Sequences upstream of -341 are not required for heat-inducible expression but are required for ovarian expression. Conversely, sequences between -351 and -53 are unnecessary for ovarian expression but are needed for maximal heat-inducible expression. We conclude that most, or all, of the cis-acting regulatory sequences required for hsp26 gene expression during oogenesis are physically separable from those required for heat-inducible expression. These results suggest that different trans-acting factors bind at different regulatory sites in the 5'-flanking region of the gene, thus activating its transcription.

The Adh gene promoters of Drosophila melanogaster and Drosophila orena are functionally conserved and share features of sequence structure and nuclease-protected sites.

“…Protected regions located immediately upstream of the proximal start of tran-VOL. 10,1990 on scription are conserved (p0 to p3), but footprints further upstream are not (p4 to p6). Two footprints are seen in the 5' leader sequence of the proximal transcript, p8 and p7; both are conserved in the two species.…”

Characterization of the shsp genes in Drosophila buzzatii and association between the frequency of Valine mutations in hsp23 and climatic variables along a longitudinal gradient in Australia

“…The sequences which regulate the tissue-specific expression and the heat shock expression of D. melanogaster hsp26 have been shown to be located in different parts of the promoter. Three regions involved in spermatocyte-specific expression are identified downstream from −352, whereas the ovarian expression is dependent on the region (−522 to −352) (Cohen and Meselson 1985;Frank et al 1992;Michaud et al 1997).…”

“…In D. melanogaster, the regulation of each of the shsps has been thoroughly investigated, either using promoter deletions to locate important regulator regions (Cohen and Meselson 1985;Riddihough and Pelham 1986;Frank et al 1992;Michaud et al 1997), or regulation by known tissue specific transcription factors (Michaud and Tanguay 2003) or search for consensus transcription factor binding sites. The expression after heat shock is regulated by the heat shock factor (HSF) binding to the heat shock element (HSE) and HSE have been found in the promoter of most hsp genes (Mestril et al 1986;Sandaltzopoulos et al 1995;Riddihough and Pelham 1986).…”

Promoter and enhancer elements from the rat elastase I gene function independently of each other and of heterologous enhancers.

“…Fusion gene constructs with either homologous or heterologous promoters have been used to localize other cis-acting regulatory elements in upstream sequences. Deletion analyses of these types of constructs demonstrate that some of the cis-acting control elements are distinct from promoter regions (6,12,13,18,19,23). These cis-acting control elements behave like tissue-specific transcriptional enhancers in that they regulate the expression of heterologous genes independently of their position or orientation (12,13,17,18,39).…”

“…The tissue specificity of expression in transgenic animals can be assayed by measuring the product of genes containing the promoter and upstream sequences of a test gene fused to a reporter gene or to a minigene (4,6,12,13,18,19,20,23,(31)(32)(33)39). Throughout this paper, DNA sequences that designate the site of transcription initiation are referred to as promoters.…”