Separable cis-acting control elements for expression of the white gene of Drosophila.

Levis, Robert; Hazelrigg, Tulle; Rubin, Gerald M.

doi:10.1002/j.1460-2075.1985.tb04108.x

Cited by 119 publications

(64 citation statements)

References 45 publications

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“…We then monitored changes of the expression of a marker gene, the mini-white gene, that is carried within the EP(3)3583 element. The miniwhite gene in P-element transformation vectors is derived from the X-linked white gene (Levis et al 1985;Pirrotta 1988). Regulatory elements necessary for high levels of white expression in the adult eyes are absent from the mini-white gene.…”

Section: Mutagenesis To Generate a Null Allele Of The Hsp27 Genementioning

confidence: 99%

The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance

et al. 2007

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Section: Mutagenesis To Generate a Null Allele Of The Hsp27 Genementioning

confidence: 99%

The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance

et al. 2007

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“…The P}w ÷ ry + ]64B insertion strain was kindly provided by Bob Levis (Levis et al 1985). The original strain contained an unlinked lethal that was recombined out by constructing a homozygous w; P[w + ry+]64B h thcu sr e strain.…”

Section: Strainsmentioning

confidence: 99%

Masquerade: a novel secreted serine protease-like molecule is required for somatic muscle attachment in the Drosophila embryo.

Murugasu-Oei¹,

Rodrigues²,

Yang³

et al. 1995

Genes Dev.

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Diverse developmental processes, such as neuronal growth cone migration and cell shape changes, are mediated by the interactions of cells with the extracellular matrix. We describe here a secreted molecule encoded by the Drosophila masquerade (mas) gene. Total loss of mas function causes defective muscle attachment. This mutant phenotype suggests that mas normally acts to stabilize cell-matrix interaction and represents a novel functional and limiting component in the adhesion process, mas encodes a 1047-amino-acid preproprotein that is further processed by proteolytic cleavage to generate two polypeptides. The carboxy-terminal polypeptide is highly similar to serine proteases and has an extracellular localization; however, it is unlikely to possess proteolytic activity, because the catalytic site serine has been substituted by a glycine residue. During embryonic development, the mas amino-and carboxy-terminal polypeptides are differentially localized. The mas carboxy-terminal polypeptide accumulates at all somatic muscle attachment sites, which corresponds well with the morphological defect seen in the mas mutants. Our findings demonstrate the involvement of an extracellular component in somatic muscle attachment. We propose that mas acts via its modified serine protease motif, either as a novel adhesion molecule and/or as a competitive antagonist of serine proteases, to stabilize muscle attachment.

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“…Studies of other tissue-specific genes provide evidence for a modular arrangement of distinct regulatory elements that mediate expression in different tissues. For instance, the tissuespecific expression of the Drosophila melanogaster white gene is independently controlled by different regulatory elements that are active in either the testes, the Malpighian tubules, or the eye (Levis et al 1985).…”

Section: Pollen-and Cell Type-specific Regulatory Elements Modulate Lmentioning

confidence: 99%

Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements.

Twell

Yamaguchi²,

Wing³

et al. 1991

Genes Dev.

291

220

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We have investigated the functional organization and properties of cis regulatory elements in the promoter regions of two genes from tomato (LAT52 and LAT59) that are preferentially and coordinately expressed during pollen maturation. Promoter deletion analysis in transgenic plants demonstrated that only minimal (~200 bp) promoter proximal regions are required for developmentally regulated expression in pollen and in specific cell types of the sporophyte. Cis-acting regulatory regions of these two promoters and of a third pollen-expressed promoter (LAT56) were characterized in detail using a transient expression assay. We identified two upstream activator regions in the LAT52 promoter and further showed that a 19-bp segment from one of those regions enhanced expression of the heterologous CaMV35S promoter in pollen. Similarities in sequence between crucial cis elements provide evidence that shared regulatory elements are involved in the coordinate expression of the LAT genes during microsporogenesis.[Key Words: Tomato; tobacco; male gametophyte; transcriptional control; enhancer; cell-specific gene regulation]Received September 28, 1990; revised version accepted January 4, 1991.Identifying the molecular components that control the spatial and temporal patterns of gene expression accompanying gametogenesis is an important step in understanding sexual reproduction in eukaryotes. We are interested in the development of the male gametophyte (pollen) of angiosperms because it provides a unique system with which to study cell-specific gene regulation and cellular differentiation during reproductive development in plants. Major cytological and biochemical events that occur during pollen development are well characterized, the mRNA populations in pollen have been analyzed, pollen-specific isoenzymes have been identified, and several genes that are preferentially expressed in pollen in a stage-specific manner recently have been cloned and sequenced (for reviews, see Mascarenhas 1975Mascarenhas , 1989. Of particular interest is the asymmetric mitotic division of the haploid microspore (microspore mitosis), which results in the formation of two dimorphic cells with different developmental fates. The Present addresses: ~Leicester Biocentre, University of Leicester, Leicester LE1 7RH UK;

show abstract

Separable cis-acting control elements for expression of the white gene of Drosophila.

Cited by 119 publications

References 45 publications

The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance

The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance

Masquerade: a novel secreted serine protease-like molecule is required for somatic muscle attachment in the Drosophila embryo.

Promoter analysis of genes that are coordinately expressed during pollen development reveals pollen-specific enhancer sequences and shared regulatory elements.

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