Seonamhaeicola aphaedonensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a tidal flat sediment

Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, AH-M5T, which was isolated from a tidal flat sediment at Aphae Island in South Korea, was characterized taxonomically. Strain AH-M5T grew optimally at 25 °C, at pH 7.0–8.0 and in presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain AH-M5T clustered coherently with the type strains of Mangrovimonas yunxiaonensis and Meridianimaribacter flavus , showing 93.4–94.3 % sequence similarity. The novel strain exhibited 16S rRNA gene sequence similarity values of less than 93.4 % to the type strains of other recognized species. Strain AH-M5T contained MK-6 as the predominant menaquinone and iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) as the major fatty acids. The polar lipid profile of strain AH-M5T containing phosphatidylethanolamine and one unidentified lipid as major components was differentiated from those of the type strains of Mangrovimonas yunxiaonensis and Meridianimaribacter flavus . The DNA G+C content of strain AH-M5T was 34.8 mol%. Differential phenotypic properties, together with the phylogenetic and chemotaxonomic data, demonstrated that strain AH-M5T is distinguished from Mangrovimonas yunxiaonensis and Meridianimaribacter flavus . On the basis of the data presented, strain AH-M5T is considered to represent a novel genus and species within the family Flavobacteriaceae , for which the name Seonamhaeicola aphaedonensis gen. nov., sp. nov. is proposed. The type strain of the type species is AH-M5T ( = KCTC 32578T = CECT 8487T).

Seonamhaeicola algicola sp. nov., a complex-polysaccharide-degrading bacterium isolated from Gracilaria blodgettii, and emended description of the genus Seonamhaeicola

“…T were similar to those of S. aphaedonensis AH-M5 T , as they both contained phosphatidylethanolamine and an unknown lipid (Park et al, 2014). Consequently, strain Gy8 T and S. aphaedonensis AH-M5…”

“…S1). The polar lipid profile of strain Gy8 T distinguished it from S. aphaedonensis AH-M5 T and J. pallidilutea EM39 T by having three additional unknown lipids and by the absence of glycolipid (Park et al, 2013(Park et al, , 2014). However, the major polar lipids of strain Gy8…”

“…Growth occurs at 10-40 uC (optimum, 30-37 uC), at pH 6.0-8.0 (optimum, 7.0-7.5) and with 0-7.0 % (w/v) NaCl (optimum, 2.0-2.5 % Park et al (2014) and Lee et al (2009).…”

“…The genus Seonamhaeicola belongs to the family Flavobacteriaceae, phylum Bacteroidetes, and was first proposed by Park et al (2014). Members of this genus contain MK-6 as the major menaquinone and phosphatidylethanolamine and an unknown lipid as the major polar lipids.…”

Seonamhaeicola acroporae sp. nov., a marine species of the family Flavobacteriaceae isolated from the hard coral Acropora formosa

“…Members of family Flavobacteriaceae (Jooste 1985) within the phylum Bacteroidetes (Garrity and Holt 2001) have been isolated and identified from many diverse marine environments and marine organisms (Kirchman 2002;O'Sullivan et al 2006;Alonso et al 2007;Zeng et al 2013). The genus Seonamhaeicola, a marine member of the family Flavobacteriaceae, was first proposed by Park et al (2014). Recently, the description of the genus has been amended (Zhou et al 2016).…”