Senyawa Sitotoksik dari Fraksi Diklorometana Kulit Terong Ungu (Solanum melongena L.) Terhadap Sel Kanker Payudara T47D

Widiyantoro, Ari; Harlia, Harlia; Prasetya, Banu

doi:10.33751/jf.v11i2.3110

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Perbedaan nilai IC50 dari ekstrak metanol, fraksi n-heksana, fraksi etil asetat, dan fraksi metanol diprediksi akibat adanya perbedaan distribusi metabolit sekundernya yang terjadi karena perbedaan kepolaran ekstrak dari masing-masing fraksi. Kondisi ini memungkinkan terjadinya interaksi sinergis dan antagonis senyawa-senyawa yang terkandung, sehingga mempengaruhi aktivitas sitotoksik terhadap sel kanker (Prasetya et al, 2021).…”

Section: Pembahasan Sitotoksisitas Ekstrak Dan Fraksi Terhadap Sel Ka...unclassified

Sitotoksisitas dan Selektivitas Fraksi Kayu Batang Simpur Air (Dillenia suffruticosa (Griff.) Martelli) Terhadap Sel Kanker Payudara

Nurdiani,

Masriani,

Rasmawan

et al. 2023

Al-Kauniyah J. Biol.

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AbstrakKanker payudara didiagnosis menjadi penyebab utama kematian pada wanita di seluruh dunia. Beberapa penelitian terdahulu telah membuktikan bahwa tanaman simpur air (Dillenia suffruticosa (Griff.) Martelli) dapat menjadi agen antikanker yang potensial. Penelitian ini bertujuan untuk menentukan sitotoksisitas dan selektivitas dari fraksi kayu batang simpur air terhadap sel kanker payudara T47D, MCF-7, dan 4T1. Metode penelitian adalah eksperimental laboratorium dan uji aktivitas antikanker terhadap sel kanker payudara dilakukan secara in vitro menggunakan sel kanker payudara T47D, MCF-7, dan 4T1. Proses ekstraksi kayu batang simpur air menggunakan metode maserasi dengan pelarut metanol, selanjutnya difraksinasi dengan pelarut n-heksana dan etil asetat secara berturut-turut dan diuji aktivitas sitotoksiknya dengan metode MTT [3-(4,5-dimetiltiazol-2-il)-2,5 difeniltetrazolium bromida] assay. Hasil yang diperoleh, yaitu fraksi n-heksana menunjukkan sifat sitotoksik sedang pada sel kanker payudara T47D, MCF-7, dan 4T1 dengan nilai IC50 berturut-turut sebesar 65,27 ± 26,17; 66,98 ± 0,97; dan 69,23 ± 6,25 µl/mL dengan IS>2. Dengan demikian, fraksi n-heksana kayu batang simpur berpotensi dikembangkan sebagai antikanker baru.AbstractBreast cancer is diagnosed as the leading cause of death in women worldwide. Several previous studies have proven that the simpur air plant (Dillenia suffruticosa (Griff.) Martelli) can be a potential anticancer agent. This study aims to determine the cytotoxicity and selectivity of the simpur air stem wood fraction against T47D, MCF-7, and 4T1 breast cancer cells. This research is laboratory experimental and the anticancer activity test against breast cancer cells was carried out in vitro. The extraction process for Simpur Air wood stems uses the maceration method with methanol solvent, then fractionated with n-hexane and ethyl acetate solvents successively and tested for cytotoxic activity using the MTT method [3-(4,5-dimethylthiazol-2-yl)-2 .5 diphenyltetrazolium bromide] assay. The results obtained, namely the n-hexane fraction showed moderate cytotoxic properties on T47D, MCF-7, and 4T1 breast cancer cells with IC50 values of 65.27 ± 26.17 respectively; 66.98 ± 0.97; and 69.23 ± 6.25 µl/mL with IS>2. Thus, the n-hexane fraction of simpur stem wood has the potential to be developed as a new anticancer.

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Section: Pembahasan Sitotoksisitas Ekstrak Dan Fraksi Terhadap Sel Ka...unclassified

Sitotoksisitas dan Selektivitas Fraksi Kayu Batang Simpur Air (Dillenia suffruticosa (Griff.) Martelli) Terhadap Sel Kanker Payudara

Nurdiani,

Masriani,

Rasmawan

et al. 2023

Al-Kauniyah J. Biol.

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“…Analisis senyawa flavonoid menggunakan reagen Serium Sulfat yang ditandai dengan terbentuknya noda berwarna jingga hingga coklat (Meidianto, et al, 2019). Noda berwarna merah jingga hingga coklat terbentuk karena terjadi reaksi redoks karena senyawa flavonoid teroksidasi dari gugus karboksilat menjadi keton, kemudian serium (IV) sulfat (Ce(SO4)2 ) tereduksi yang semula Ce 4+ menjadi Ce 3+ sehingga menimbulkan warna jingga-coklat [7].…”

Section: Maserasi Dan Partisiunclassified

Anti-Termite Activities of The Bioactive Compounds of Gaharu Culture (Aetoxylon sympetalum) From Maceration Results Using Acetone Solvent

Yuniar,

Jayuska,

Alimuddin

et al. 2023

B. SAINSTEK

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Agarwood is a plant known for its aromatic resin, which is one of the most widely distributed species in Indonesia. This research was carried out in several stages, namely maceration, partitioning, Thin Layer Chromatography (TLC), and termites activity. The test was carried out for 3 days with variations in the concentration of 0% (negative control), 0.1%, 0.2%. 0.3%, 0.4%, 0.5% (w/v), and 0.25% fipronil reagent as positive controls. The crude extract yield of sapwood gaharu aloe acetone obtained was 23,4321% and the partition result of 233,789 grams of crude extract of acetone consisted of n-hexane fraction with a yield of 3.812%, chloroform fraction of 42.205%, and acetone fraction of 43.621%. The results of the phytochemical test showed that aloe vera sapwood contained alkaloid compounds, flavonoids, steroids, terpenoids, phenolics and saponins. GC-MS analysis of the n-hexane fraction showed the number of peaks as many as 55 peaks which may contain 55 compounds in the fraction. The compound with the highest % area was 4-Chloro-6-methoxy-2-methyl quinoline-8-amine (10.33%) followed by stigmasterol compound (5.94%). The results of the termite activity test showed that the most active fraction as an anti-termite was acetone fraction (LC50 0.082%) followed by chloroform fraction (LC50 0.134%), crude extract (LC50 0.144%) and n-hexane fraction (LC50 0.176%)

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Senyawa Sitotoksik dari Fraksi Diklorometana Kulit Terong Ungu (Solanum melongena L.) Terhadap Sel Kanker Payudara T47D

Cited by 2 publications

References 3 publications

Sitotoksisitas dan Selektivitas Fraksi Kayu Batang Simpur Air (Dillenia suffruticosa (Griff.) Martelli) Terhadap Sel Kanker Payudara

Sitotoksisitas dan Selektivitas Fraksi Kayu Batang Simpur Air (Dillenia suffruticosa (Griff.) Martelli) Terhadap Sel Kanker Payudara

Anti-Termite Activities of The Bioactive Compounds of Gaharu Culture (Aetoxylon sympetalum) From Maceration Results Using Acetone Solvent

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