Sentinel node mapping by colonic tattooing

Ra, Chisei; Lindsey, Ian; Cunningham, Chris

doi:10.1007/s00464-010-0941-1

Cited by 8 publications

(10 citation statements)

References 6 publications

(6 reference statements)

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“…Furthermore, the multivariate analysis of OSNA results showed that the number of collected LN, the gender and the histologic grade were independent predictors of OSNA results. Our data highlights the importance of procuring any identifiable LN irrespective of its size [ 40 – 42 , 45 – 47 ].…”

Section: Discussionmentioning

confidence: 95%

Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study

et al. 2016

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Stage I–II (pN0) colorectal cancer patients are surgically treated although up to 25 % will eventually die from disease recurrence. Lymph node (LN) status is an independent prognostic factor in colorectal cancer (CRC), and molecular tumour detection in LN of early-stage CRC patients is associated with an increased risk of disease recurrence and poor survival. This prospective multicentre study aimed to determine the relationship between LN molecular tumour burden and conventional high-risk factors in stage I–II colon cancer patients. A total of 1940 LN from 149 pathologically assessed pN0 colon cancer patients were analysed for the amount of tumour cytokeratin 19 (CK19) messenger RNA (mRNA) with the quantitative reverse transcription loop-mediated isothermal amplification molecular assay One-Step Nucleic Acid Amplification. Patient’s total tumour load (TTL) resulted from the sum of all CK19 mRNA tumour copies/μL of each positive LN from the colectomy specimen. A median of 15 LN were procured per case (IQR 12;20). Molecular positivity correlated with high-grade (p < 0.01), mucinous/signet ring type (p = 0.017), male gender (p = 0.02), number of collected LN (p = 0.012) and total LN weight per case (p < 0.01). The TTL was related to pT stage (p = 0.01) and tumour size (p < 0.01) in low-grade tumours. Multivariate logistic regression showed independent correlation of molecular positivity with gender, tumour grade and number of fresh LN [AUC = 0.71 (95 % CI = 0.62–0.79)]. Our results show that lymph node CK19 mRNA detection correlates with classical high-risk factors in stage I–II colon cancer patients. Total tumour load is a quantitative and objective measure that may help to better stage early colon cancer patients.Electronic supplementary materialThe online version of this article (doi:10.1007/s00428-016-1990-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 95%

Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study

et al. 2016

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“…24–27 In the current study, multiple patients received pre-operative endoscopic tattooing of the tumor, which resulted in multiple blue colored nodes prior to the SLN procedure. Since the difference between staining by endoscopic tattooing and patent blue appeared to be difficult, the blue dye staining could be less reliable for detecting the first echelon nodes in the current study.…”

Section: Discussionmentioning

confidence: 99%

Ex vivo sentinel node mapping in colon cancer combining blue dye staining and fluorescence imaging

Schaafsma

Verbeek

Vorst

et al. 2013

Journal of Surgical Research

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Background The sentinel lymph node procedure has been proposed to improve nodal staging in colon cancer patients. The aim of this study was to assess the added value of near-infrared fluorescence imaging to conventional blue dye staining for ex vivo sentinel lymph node mapping. Materials and Methods Twenty-two consecutive patients undergoing surgery for colon cancer were included. After tumor resection, a premixed cocktail of the near-infrared lymphatic tracer HSA800 and blue dye was submucosally injected around the tumor for detection of sentinel lymph nodes. The Mini-FLARE imaging system was used for fluorescence imaging. Results In 95% of the patients, at least one sentinel lymph node was identified. Overall, a total of 77 sentinel lymph nodes were identified, of which 77 were fluorescent (100%) and 70 (91%) were blue. Sentinel lymph nodes that were located deeper in the mesenteric fat could easily be located by NIR fluorescence. In 4 out of 5 patients with lymph node metastases, tumor cells were present in at least 1 of the sentinel lymph nodes. Conclusions This study shows the successful use and added value of the near-infrared fluorescence tracer HSA800 to conventional blue dye for the ex vivo sentinel lymph node procedure in colon cancer.

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“…There is no definite proof yet that the carboncontaining LNs are indeed the first echelon nodes, but it does seem very plausible that the greatest degree of ink is deposited in the first order draining nodes. 3 However, secondary echelon nodes cannot be discriminated from first echelon nodes with this technique. This probably would have resulted in a higher number of detected sentinel nodes.…”

Section: Increased Ln Yieldmentioning

confidence: 99%

The effect of colonoscopic tattooing on lymph node retrieval and sentinel lymph node mapping

Bartels¹,

Zaag²,

Dekker³

et al. 2012

Gastrointestinal Endoscopy

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Sentinel node mapping by colonic tattooing

Cited by 8 publications

References 6 publications

Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study

Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study

Ex vivo sentinel node mapping in colon cancer combining blue dye staining and fluorescence imaging

The effect of colonoscopic tattooing on lymph node retrieval and sentinel lymph node mapping

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