Sentinel-node biopsy sampling in breast cancer

Jones, Peter A.; Jones, Sue; Humphreys, S.; Schofield, J. B.; Pickering, D. C.

doi:10.1016/s0140-6736(05)73829-0

Cited by 3 publications

(1 citation statement)

References 3 publications

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“…As a consequence, depending on the number of sections per node examined, the diagnosis can be given with unsurpassed reliability. Since there is at present some controversy about sentinel nodebased decisions in surgery [11,16], we would argue that a better methodology of nodal examinations, and especially more dependable NO diagnoses could lead to better results with the application of this surgical concept. From the technical point of view, the proposed procedure proved to be simple, fast, sensitive and very satisfying.…”

Section: Counterstainingmentioning

confidence: 99%

Fast and sensitive immunodetection of carcinoma cells in sentinel nodes

Karsten

Stosiek

2001

Virchows Arch

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In a number of clinical situations, especially in the context of the recent sentinel node concept, lymph-node involvement has to be determined intraoperatively. Since serious and dependable decisions are to be made according to the result of this examination, the most reliable method for the detection of tumour cells should be applied. We and others have shown previously that routine histological examination underestimates lymph-node metastases, and that immunohistochemistry (IHC) significantly improves the accuracy of staging. However, IHC has so far been difficult to apply to the intraoperative examination of cryosections since it has required too much time. We have developed a novel modification of IHC for the rapid detection of metastases of carcinomas in cryosections from lymph nodes. It is based on a unique directly labelled cytokeratin antibody, immunofluorescence, and a specially devised staining solution. This one-step staining procedure can be performed within 10 min. At the same time, its sensitivity is very high. Single tumour cells can easily be detected, and background staining is very low. The high sensitivity could result in a markedly improved reliability of sentinel node-based decisions.

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Section: Counterstainingmentioning

confidence: 99%