Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin

Abstract

Patent Blue V (PBV), a dye used clinically for sentinel lymph node detection, was mixed with human serum albumin (HSA). After binding to HSA, the fluorescence quantum yield increased from 5 × 10−4 to 1.7 × 10−2, which was enough to allow fluorescence detection and imaging of its distribution. A detection threshold, evaluated in scattering test objects, lower than 2.5 nmol × L−1 was obtained, using a single-probe setup with a 5-mW incident light power. The detection sensitivity using a fluorescence imaging device was in the µmol × L−1 range, with a noncooled CCD camera. Preclinical evaluation was performed on a rat model and permitted to observe inflamed nodes on all animals.

Health safety issues of synthetic food colorants

“…Furthermore, new toxicity concerns have arisen in several colorants due to their ability to bind to human serum albumin. This was reported in Sunset Yellow (Kus and Eroglu, 2015), Tartrazine (Pan et al, 2011), Azorubine (Basu and Kumar 2014a;Datta et al, 2013), Allura Red (Wang et al, 2014;Wu et al, 2015a) and Patent Blue (Tellier et al, 2012). Hypersensitivity reactions have also been reported to become more frequent with new indications of the dyes, e.g.…”

“…Specifically, binding to HSA was reported for Tartrazine (Pan et al, 2011), Azorubine (Basu and Kumar 2014a;Datta et al, 2013;Masone and Chanforan, 2015), Allura Red (Masone and Chanforan, 2015;Wang et al, 2014;Wu et al, 2015a) Sunset Yellow, Quinoline Yellow (Masone andChanforan, 2015), and Patent Blue (Tellier et al, 2012). This indicates a potential for drugedye interactions.…”

Surface Chemistry Architecture of Silica Nanoparticles Determine the Efficiency of in Vivo Fluorescence Lymph Node Mapping

“…5À9 In this context, the diagnostic potential of NP-based NIR contrast agents is particularly appreciated considering the deep tissue penetration of NIR radiation and the minimal induced tissue autofluorescence. 10 Several NIR emitting tracers, such as indocyanine green (ICG), 11 Patent Blue V, 12 silicon dots, 13 or quantum dots, 14 have already been used as lymphatic tracers for sentinel lymph node (SLN) detection in preclinical models. However, many properties such as size, architecture, and toxicity of these tracers are not appropriate and need to be improved.…”

Red blood cell transport mechanisms in polyester thread-based blood typing devices

“…The three zones can be seen in Fig. 1d, and are: 1. a RBC dosing zone, which had the deep crimson colour of blood because of the presence of a large number of RBCs; 2. a yellowish separation zone, where it was hypothesised that plasma had separated from the whole blood and wicked further along the thread; and 3. a dry zone which no blood or plasma had reached, which remained coloured only by the non-fluorescent blue dye [30], Patent Blue, added to the antibody solution for identification purposes.…”