Sentinel Lymph Node Mapping in Melanoma with Technetium-99m Dextran

Neubauer, Stefan; Mena, Iván X.; Iglesis, Rodrigo; Schwartz, R. D.; Acevedo, Juan Carlos; Leon, A.; Gómez, Luis Fiter

doi:10.1089/10849780152389447

Cited by 9 publications

(6 citation statements)

References 9 publications

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“…[43][44][45] In addition, paramagnetic or radiolabeled polysacchrides have been applied for diagnostic imaging. [46][47][48][49][50][51][52][53][54] Another notable feature of polysaccharide is to compose different sugars that can be recognized by the respective cell receptors of sugar specificity. This biological recognition not only permits the receptor-specific targeting of agents to the cell but also accelerates their cell uptake via the receptor-mediated endocytosis.…”

Section: Introductionmentioning

confidence: 99%

Promoted Adipogenesis of Rat Mesenchymal Stem Cells by Transfection of Small Interfering RNA Complexed with a Cationized Dextran

Nagane

Tabata

2010

Tissue Engineering Part A

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The objective of this study is to investigate the possibility of small interfering RNA (siRNA) complexed with a cationized dextran of nonviral carrier to biologically modify the adipogenesis extent of bone marrow-derived mesenchymal stem cells (MSC). Spermine was chemically introduced to the hydroxyl groups of dextran to prepare the cationized dextran (spermine-dextran). The spermine-dextran could form a complex with siRNA, and the physicochemical properties were changed by the molecular weight of dextran, the molar percentage of spermine introduced to dextran, and the molar ratio of nitrogen molecule of spermine-dextran to the phosphorous ones of siRNA (N/P ratio). The gene expression level of luciferase or green fluorescence protein was significantly suppressed by transfection with the complex of spermine-dextran and siRNA. The gene expression level by the complex decreased with an increase in the extent of complex internalized. Biochemical experiments revealed that culture in an adipogenic differentiation medium allowed MSC to differentiate into adipogenic cells. However, upon culturing with siRNA of anti-transcription coactivator containing PDZ-binding motif (TAZ) for osteogenic differentiation complexed with the spermine-dextran, the adipogenesis of MSC was further promoted. It is concluded that the spemine-dextran was a promising nonviral carrier to suppress the expression level of differentiation gene, resulting in the modification of cell differentiation direction.

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Section: Introductionmentioning

confidence: 99%

Promoted Adipogenesis of Rat Mesenchymal Stem Cells by Transfection of Small Interfering RNA Complexed with a Cationized Dextran

Nagane

Tabata

2010

Tissue Engineering Part A

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“…BENNETT & LAGO (4) claimed that good results were obtained using dextran 500 99m Tc in head and neck melanoma, while NEUBAUER et al (13) described equal with dextran 500 in cutaneous melanomas.…”

Section: Discussionmentioning

confidence: 99%

Phytate technetium-99m versus dextran 500 technetium-99m in the sentinel lymph node biopsy

et al. 2006

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“…Unexpectedly, in each of these patients lymphoscintigraphy found a SLN in both the contralateral and ipsilateral axillae. Drainage pathways on the trunk are known to be complex and unpredictable 14–17. In the patients that followed, ultrasound in truncal melanoma was extended to both axillae as well as both inguinal areas.…”

Section: Discussionmentioning

confidence: 99%

Preoperative ultrasonographic identification of the sentinel lymph node in patients with malignant melanoma

Kahle¹,

Hoffend²,

Wacker³

et al. 2003

Cancer

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BACKGROUND Dissection of the “sentinel lymph node” (SLN) as identified by lymphoscintigraphy is becoming increasingly important in the treatment of patients with malignant melanoma. The purpose of the current study was to determine whether the SLN also could be identified by ultrasound. METHODS Sixty‐seven patients with malignant melanoma (40 females and 27 males, with an average age of 48.8 years) in whom extirpation of the SLN was indicated underwent ultrasonography of the regional lymph nodes prior to preoperative lymphoscintigraphy. The location of the melanoma was the legs in 30 patients, the arms in 14 patients, and the trunk in 23 patients. During regional ultrasonography, the location of the lymph nodes that differed in the cortex/medulla ratio from the surrounding lymph nodes was marked on the skin corresponding to the planes of insonation (M1) when the probe was held vertically to the skin surface. After lymphoscintigraphy using technetium‐99m, the position of a gamma probe at which the highest count rate vertical to the skin surface was recorded also was marked (M2). RESULTS In the inguinal region, the agreement between M1 and M2 was found to be 100% (40 of 40 SLNs) and was 72.5% in the axilla (29 of 40 SLNs). In patients with melanomas located on the leg, the location of M1 and M2 agreed in 97% of cases (36 of 37 lymph nodes in 30 patients); in patients with melanomas located on the arms, the agreement was 76% (13 of 17 lymph nodes in 14 patients) and in patients with melanomas located on the trunk, the agreement was 75% (21 of 28 lymph nodes in 23 patients). The position documented by ultrasound relative to the neighboring structures of the SLN was confirmed intraoperatively in all cases. CONCLUSIONS The results of the current study indicate that the SLN in patients with melanoma located on the limbs, especially the legs, are characterized by a specific sonomorphologic pattern. Preoperative sonography might constitute an important addition to lymphoscintigraphy in the planning of SLN biopsy. Cancer 2003;97:1947–54. © 2003 American Cancer Society. DOI 10.1002/cncr.11261

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Sentinel Lymph Node Mapping in Melanoma with Technetium-99m Dextran

Abstract: Tc99m-Dextran provided good definition of lymph channels and sentinel node localization, without the risks related to the use of potentially hazardous labeled materials of biological origin.

Cited by 9 publications

References 9 publications

Promoted Adipogenesis of Rat Mesenchymal Stem Cells by Transfection of Small Interfering RNA Complexed with a Cationized Dextran

Promoted Adipogenesis of Rat Mesenchymal Stem Cells by Transfection of Small Interfering RNA Complexed with a Cationized Dextran

Phytate technetium-99m versus dextran 500 technetium-99m in the sentinel lymph node biopsy

Preoperative ultrasonographic identification of the sentinel lymph node in patients with malignant melanoma

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