Sentinel Lymph Node Biopsy in Cutaneous Melanoma: The WHO Melanoma Program Experience

Cascinelli, Natale; Belli, Filiberto; Santinami, Mario; Fait, Vuk; Testori, Alessandro; Ruka, W.; Cavaliere, R.; Mozzillo, Nicola; Róssi, Carlo; MacKie, Rona M.; Nieweg, Omgo E.; Pace, M; Kirov, K.

doi:10.1007/s10434-000-0469-z

Cited by 293 publications

(172 citation statements)

References 15 publications

Supporting

Mentioning

138

Contrasting

Unclassified

Order By: Relevance

“…This could be explained in part by the short followup period of our study. In contrast to this assumption, two other studies by Nowecki and Cascinelli with similar follow-up periods of 34 and 29 months, respectively, all showed a significant impact of SLN status on overall survival upon multivariate analysis (Cascinelli et al, 2000;Nowecki et al, 2003). Furthermore, Meier demonstrated that distant metastasis in patients with prior metastasis to lymph nodes already evolves after a median followup period of 25 months (Meier et al, 2002).…”

Section: Discussionmentioning

confidence: 92%

See 1 more Smart Citation

Sentinel node status in melanoma patients is not predicitive for overall survival upon multivariate analysis

et al. 2005

View full text Add to dashboard Cite

Sentinel lymph node biopsy (SLNB) has become a widely accepted standard procedure in the staging of patients with cutaneous melanoma and absence of clinical lymph node metastases, although there is no final proof that SLNB influences overall survival in these patients. This study investigated the accuracy of SLNB and the clinical outcome of patients after a mean follow-up of 22 months. Between 1998 and 2003, SLNB was performed in 309 consecutive patients. Patients with one or more positive sentinel lymph nodes (SLNs) were subjected to selective lymphadenectomy (SL). Survival analyses were performed using the Kaplan -Meier approach. A Cox proportional-hazard analysis was used for univariate and multivariate analysis to explore the effect of variables on survival. Sentinel lymph nodes were identified in 299 of 309 patients (success rate: 96.8%). Of these, 69 (23%) had a positive SLN. The falsenegative rate was 9.2%. Recurrence of disease to the regional lymph node basin (3.0%) and to the locoregional skin (2.6%) was rare in SLN-negative patients in contrast to SLN-positive patients (7.2 and 17.4%, respectively). The 3-year overall survival was 93 and 83% for SLN-negative and SLN-positive patients, respectively. Upon multivariate analysis, SLN status (Po0.001), Breslow thickness (Po0.02) and ulceration (Po0.026) were all found to be independent prognostic factors with respect to disease-free survival, whereas Breslow thickness proved to be the only significant factor with respect to overall survival.

show abstract

Section: Discussionmentioning

confidence: 92%

“…Lymphoscintigraphy according to Berger and Cascinelli (Berger et al, 1997;Cascinelli et al, 2000) was performed the day before surgery by intradermal administration of technetium-99m-labelled nanocolloid at a dose of 30 -60 MBq (Nanocoll s ; Sorin Biomedica, Saluggia, Italy) around the biopsy site. Dynamic and static images were obtained.…”

Section: Methodsmentioning

confidence: 99%

Sentinel node status in melanoma patients is not predicitive for overall survival upon multivariate analysis

et al. 2005

View full text Add to dashboard Cite

show abstract

“…When this combined approach is used, 98% of sentinel nodes are successfully identified in our department which corresponds favourably with the published literature (6)(7)(8)(9)(10)(11) . Sentinel lymph node biopsy has been studied most extensively in the context of malignant melanoma (12) .…”

Section: Sentinel Lymph Node Biopsy (Fig-2)mentioning

confidence: 99%

Surgical Management of M etastatic groin lymphadenopathyRetrospective study of 50 Cases in Regional Cancer Center

Dasappa¹

2018

jmscr

View full text Add to dashboard Cite

show abstract

“…The first protocol is based on cutting the sentinel lymph node into 1-3 mm thick slices. 11 A certain number of microtome sections from each slice is stained by H&E and immunohistochemistry.…”

mentioning

confidence: 99%

Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients

et al. 2009

View full text Add to dashboard Cite

The optimal processing for the pathology of sentinel lymph nodes of patients with melanoma is still a matter of debate. We compared two protocols of sentinel lymph node processing, which were consecutively applied. For the first protocol, the sentinel lymph nodes were cut into 1-2 mm thick slices. From each slice, 12 microtome sections were stained (multiple slices protocol). For the second protocol, which is a modification of the recent European Organisation for Research and Treatment of Cancer protocol, the sentinel lymph nodes were bivalved. Five consecutive series of microtome sections, with gaps of 50 lm between them, were prepared from each cut surface (bivalving protocol). H&E and immunohistochemical staining were integral elements of both protocols. A total of 584 sentinel lymph nodes (1.8±0.9 per patient) were examined. The percentages of micrometastases (29 versus 27%) and of capsular naevi (13 versus 15%) detected were very similar for both protocols. As shown by multivariate logistic regression, Breslow thickness (P ¼ 0.003) and younger age (P ¼ 0.01) correlated with nodal metastasis. The type of histological preparation, ulceration and sex were not significant. The multiple slices protocol produced, on average, 4 paraffin blocks and 46 microtome sections per node. The bivalving protocol constantly produced 2 paraffin blocks and 42 microtome sections. For technical processing, the multiple slices protocol required, on average, 38 min per sentinel lymph node, whereas the bivalving protocol required 55 min. Both protocols yielded excellent detection rates with a similar amount of work being required on the part of the pathologist. Compared with the bivalving protocol, the multiple slices protocol was less labor intensive for the technical staff.

show abstract

Sentinel Lymph Node Biopsy in Cutaneous Melanoma: The WHO Melanoma Program Experience

Cited by 293 publications

References 15 publications

Sentinel node status in melanoma patients is not predicitive for overall survival upon multivariate analysis

Sentinel node status in melanoma patients is not predicitive for overall survival upon multivariate analysis

Surgical Management of M etastatic groin lymphadenopathyRetrospective study of 50 Cases in Regional Cancer Center

Cutting a sentinel lymph node into slices is the optimal first step for examination of sentinel lymph nodes in melanoma patients

Contact Info

Product

Resources

About