Sensory transducers of E. coli are encoded by homologous genes

Boyd, Alan; Krikos, Alexandra

doi:10.1016/0092-8674(81)90202-6

Cited by 63 publications

(39 citation statements)

References 32 publications

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“…The two domains of these methyl-accepting chemotaxis proteins (MCPs; Mr = 58,000 to 60,000) are connected by a single hydrophobic transmembrane helix of -24 residues. These structural features have been deduced from a limited number of nucleotide sequences of genes for MCPs from members of the family Enterobacteriaceae, primarily the Escherichia coli tap, tar, tsr, and trg genes (4,5,14) and the Salmonella typhimurium tar gene (26). There is good experimental evidence that MCPs function more generally in motile prokaryotes in transduction of outside chemical and physical signals to the flagellar motor.…”

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confidence: 99%

Nucleotide sequence of dcrA, a Desulfovibrio vulgaris Hildenborough chemoreceptor gene, and its expression in Escherichia coli

Dolla

Brumlik

et al. 1992

J Bacteriol

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The amino acid sequence of DcrA (Mr = 73,000), deduced from the nucleotide sequence of the derA gene from the anaerobic, sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough, indicates a structure similar to the methyl-accepting chemotaxis proteins from Escherichia coli, including a periplasmic NH2-terminal domain (Mr = 20,700) separated from the cytoplasmic COOH-terminal domain (Mr = 50,300)

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confidence: 99%

Nucleotide sequence of dcrA, a Desulfovibrio vulgaris Hildenborough chemoreceptor gene, and its expression in Escherichia coli

Dolla

Brumlik

et al. 1992

J Bacteriol

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“…The Tar protein of Escherichia coli is one of a family of homologous chemotactic sensory transducers (4,6,19). These proteins are found in the cytoplasmic membrane and enable the bacterium to monitor its chemical surroundings.…”

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Aspartate and maltose-binding protein interact with adjacent sites in the Tar chemotactic signal transducer of Escherichia coli

et al. 1992

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“…4) (24). Functional homology is demonstrated from cross-species complementation (9,10) and because these receptors are a common substrate for the CheR methylase and CheB demethylase (28,30 (3,34). This is in sharp contrast to the similar chromosomal locations seen so far among the chemotaxis genes from E. coli and S. typhimurium (9,10).…”

Section: Methodsmentioning

confidence: 99%

“…This gene has been named tip, for taxis-involved protein, following the example set in naming the tap gene (taxis-associated protein), for which ligand specificity is also not known (3). In this report we describe the structural and functional properties of Tip receptors and compare Tip receptors with the possibly analogous Tap receptors in E. coli cells.…”

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“…The trg gene encodes a receptor which recognizes the ligand-occupied forms of the ribose-and galactosebinding proteins' (12). An additional gene with receptorlike properties, tap (taxis-associated protein) has been identified tandem to the E. coli aspartate receptor gene (3,25,34). The specificity of the tap receptor is not known, but it is not a duplicate aspartate or serine receptor gene (25).…”

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Identification of the tip-encoded receptor in bacterial sensing

Russo¹,

Koshland²

1986

J Bacteriol

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A chemotaxis gene encoding a protein with receptorlike properties has been identified in Salmonella typhimurium and termed tip for taxis-involved protein. Based on the stringency of DNA hybridization, the tip gene has about 75% homology with a region of the tar gene encoding the cytoplasmic domain of the aspartate receptor. Introduction of the tip gene into a smooth-swimming Escherichia coli receptor mutant (tar tsr tap) restored both chemotaxis ability on soft-agar-tryptone plates and a wild-type swimming phenotype. We have shown, by overexpressing the CheY protein, that shifting of the mutant swimming bias in the absence of receptors is insufficient to restore chemotaxis ability. This suggests that in addition to resetting the swimming bias, the tip gene product functions as a receptor. By functional criteria, we found that Tip is not a duplicate aspartate (Tar) or serine (Tsr) receptor gene. Based on behavioral properties, the S. typhimurium Tip receptor provides functional features similar to those of the E. coli Tap receptor.Bacteria detect and respond to a variety of chemicals in their surroundings (4,16,27). The receptor genes for several of these chemoeffectors have been identified and cloned from Escherichia coli and Salmonella typhimurium strains. The membrane-bound primary receptors for aspartate and serine are encoded by the tar and tsr genes, respectively (25,26,33). The trg gene encodes a receptor which recognizes the ligand-occupied forms of the ribose-and galactosebinding proteins' (12). An additional gene with receptorlike properties, tap (taxis-associated protein) has been identified tandem to the E. coli aspartate receptor gene (3,25,34). The specificity of the tap receptor is not known, but it is not a duplicate aspartate or serine receptor gene (25).Recently, the sequences for the tar, tsr, tap, and trg receptor genes were determined (1, 2, 18, 24). The receptor sequences show a striking degree of structural homology (1, 18). Although the different receptors from both E. coli and S. typhimurium are homologous, this identity is more pronounced in certain regions of the sequence. In the carboxyterminal domains there is greater than 75% identity between the aspartate and serine receptors, yet in the amino-terminal halves the sequences diverge to less than 25% identity. This divergence probably reflects differences in substrate specificity. Therefore, for DNA hybridization a probe covering the carboxy-terminal region would be most likely to detect receptorlike sequences. Such an approach has been used to identify several potential receptor genes in the Salmonella chromosome (D. Clegg and D. E. Koshland, Jr., unpublished observations).In the initial cloning of chemotaxis genes from S. typhimurium, DeFranco and Koshland (9) isolated an EcoRI restriction fragment which complemented the chemotactic migration of a triple-receptor mutant (RP4372) on soft-agar plates containing tryptone (A. DeFranco, Ph.D. thesis, University of California, Berkeley, 1979). This mutant lacks the aspartate'(Tar), serine ...

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Sensory transducers of E. coli are encoded by homologous genes

Cited by 63 publications

References 32 publications

Nucleotide sequence of dcrA, a Desulfovibrio vulgaris Hildenborough chemoreceptor gene, and its expression in Escherichia coli

Nucleotide sequence of dcrA, a Desulfovibrio vulgaris Hildenborough chemoreceptor gene, and its expression in Escherichia coli

Aspartate and maltose-binding protein interact with adjacent sites in the Tar chemotactic signal transducer of Escherichia coli

Identification of the tip-encoded receptor in bacterial sensing

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