Sensory nerve endings in the rat oro-facial region labeled by the anterograde and transganglionic transport of horseradish peroxidase: a new method for tracing peripheral nerve fibers

Marfurt, Carl F.; Turner, Dennis F.

doi:10.1016/0006-8993(83)91277-5

Cited by 59 publications

(29 citation statements)

References 53 publications

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“…In the present study, trigeminal sensory nerve fibers in the JE and OSE of the rat were demonstrated by the WGA-HRP anterograde transport technique, which allows the sensory nerves of known origin to be selectively and sensitively visualized (Robertson and Aldskogius, 1982;Marfurt and Turner, 1983;Chan and Byers, 1985b;Aldskogius et al, 1986). Previous investigators have used two methods in attempting to delineate sensory nerve fibers in these epithelia.…”

Section: Discussionmentioning

confidence: 99%

“…The injection ofWGA-HRP (wheat-germ agglutinin-horseradish peroxidase, Toyobo Co., Osaka, Japan) into the trigeminal ganglion was carried out as described by Marfurt and Turner (1983): The head of each animal was mounted dorsal side up on a stereotaxic apparatus. The left trigeminal ganglion was exposed on the floor ofthe middle cranial fossa as follows: A small portion of the parietal bone was removed and the underlying brain tissue aspirated.…”

Section: Methodsmentioning

confidence: 99%

“…Selective visualization of sensory nerve fibers was carried out by means of anterograde axonal transport of wheat-germ agglutininhorseradish peroxidase injectedinto the trigeminal ganglion (Marfurt and Turner, 1983;Chan and Byers, 1985a).…”

Section: Introductionmentioning

confidence: 99%

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A Light and Electron Microscopic Anterograde WGA-HRP Tracing Study on the Sensory Innervation of Junctional and Sulcular Epithelium in the Rat Molar

et al. 1992

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The sensory innervation of junctional and oral sulcular epithelium was investigated by use of anterograde transport of wheat-germ agglutinin-horseradish peroxidase from the trigeminal ganglion. By light microscopy, labeled intra-epithelial nerve fibers were observed forming a dense plexus in the apical two-thirds of the junctional epithelium, with some fibers located near the enamel space. Occasional fibers extended coronally to the sulcus bottom. By electron microscopy, labeled intra-epithelial axon terminals or varicosities were demonstrated to be in close contact with both junctional epithelial cells and neutrophils. These varicosities, which were occasionally surrounded by the cytoplasmic processes of epithelial cells or neutrophils, frequently contained large granular and small clear vesicles. In contrast to the junctional epithelium, the oral sulcular epithelium was sparsely innervated, except for the transition region between the oral sulcular epithelium and the junctional epithelium, where a dense innervation by labeled intraepithelial fibers was found. These fibers extended as far as the stratum spinosum. Electron microscopy revealed mitochondria-filled profiles of varicosities between epithelial cells. This study shows differences in the distribution and ultrastructure of sensory nerves between the junctional and oral sulcular epithelia, and further provides morphological evidence that sensory nerves in the junctional epithelium come into contact not only with epithelial cells but also with neutrophils.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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A Light and Electron Microscopic Anterograde WGA-HRP Tracing Study on the Sensory Innervation of Junctional and Sulcular Epithelium in the Rat Molar

et al. 1992

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“…In addition, many of the cells and agents with which the dental nerve fibers interact are shown. Mature teeth receive an enormous sensory innervation, most of which terminates in the odontoblast layer, predentin, and inner 0.1 mm of dentin of the crown (Byers, 1984;Byers and Kish, 1976;Byers and Nä rhi , 2002;Fearnhead, 1961;Gunji, 1982;Hildebrand et al, 1995;Maeda et al, 1985Maeda et al, , 1987Marfurt and Turner, 1983;Silverman and Kruger, 1987) (Figs. 2-5).…”

Section: Normal Innervation Of Teethmentioning

confidence: 98%

Dental neuroplasticity, neuro‐pulpal interactions, and nerve regeneration

Byers

Suzuki

Maeda

2003

Microscopy Res & Technique

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This review covers current information about the ability of dental nerves to regenerate and the role of tooth pulp in recruitment of regenerating nerve fibers. In addition, the participation of dental nerves in pulpal injury responses and healing is discussed, especially concerning pulp regeneration and reinnervation after tooth replantation. The complex innervation of teeth is highly asymmetric and guided towards specific microenvironments along blood vessels or in the crown pulp and dentin. Pulpal products such as nerve growth factor are distributed in the same asymmetric gradients as the dentinal sensory innervation, suggesting regulation and recruitment of those nerve fibers by those specific factors. The nerve fibers have important effects on pulpal blood flow and inflammation, while their sprouting and cytochemical changes after tooth injury are in response to altered pulpal cytochemistry. Thus, their pattern and neuropeptide intensity are indicators of pulp status, while their local actions continually affect that status. When denervated teeth are injured, either by pulp exposure on the occlusal surface or by replantation, they have more pulpal necrosis than occurs for innervated teeth. However, small pulp exposures on the side of denervated crowns or larger lesions in germ-free animals can heal well, showing the value of postoperative protection from occlusal trauma or from infection. Current ideas about dental neuroplasticity, neuro-pulpal interactions, and nerve regeneration are related to the overall topics of tooth biomimetics and pulp/dentin regeneration.

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“…Previous studies have shown that free horseradish peroxidase (HRP) and HRP conjugated to wheat germ agglutinin (WGA-HRP) and to choleragenoid (B-HRP) are efficient tracers for anterograde labeling of the peripheral innervation of various tissues after ganglion injection (Robertson and Aldskogius, 1982;Marfurt and Turner, 1983;Aldskogius et al, 1986;Fundin et al, 1994). These two HRP conjugates have been shown to give rise to different central labeling patterns after skin or ganglion injections (Robertson and Arvidsson, 1985;Robertson and Grant, 1985).…”

mentioning

confidence: 97%

Innervation of the hard palate in the rat studied by anterograde transport of horseradish peroxidase conjugates

Arvidsson

Fundin

Pfaller

1995

J of Comparative Neurology

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The innervation of the rat hard palate and the bordering part of the soft palate was studied after anterograde transport of horseradish peroxidase conjugated to wheat germ agglutinin (WGA-HRP) and to choleragenoid (B-HRP) in separate experiments. WGA-HRP labeling showed leakage from several types of nerve endings, whereas B-HRP did not. Both conjugates gave rise to heavy labeling of a variety of nerve endings. Intragemmal and, especially, perigemmal fibers were labeled in chemosensory corpuscles, which were most common in the medial wall of the incisive canal and in the most anterior part of the soft palate. Ruffini endings of different sizes were labeled in the incisive papilla. Other subepithelial endings forming elongated expanded profiles with medium- to large-caliber source fibers were most common in protruding parts of the palate. Labeled intraepithelial endings included Merkel endings, which were most frequent in the incisive papilla and the rugae. Other labeled profiles were medium-caliber afferents giving rise to irregular, beaded, and sometimes branched endings often located far superficially in the epithelium. Such endings were present both within and between protruding parts of the palate. Fine-caliber intraepithelial endings were labeled almost exclusively in WGA-HRP experiments.

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Sensory nerve endings in the rat oro-facial region labeled by the anterograde and transganglionic transport of horseradish peroxidase: a new method for tracing peripheral nerve fibers

Cited by 59 publications

References 53 publications

A Light and Electron Microscopic Anterograde WGA-HRP Tracing Study on the Sensory Innervation of Junctional and Sulcular Epithelium in the Rat Molar

A Light and Electron Microscopic Anterograde WGA-HRP Tracing Study on the Sensory Innervation of Junctional and Sulcular Epithelium in the Rat Molar

Dental neuroplasticity, neuro‐pulpal interactions, and nerve regeneration

Innervation of the hard palate in the rat studied by anterograde transport of horseradish peroxidase conjugates

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