Sensory innervation of rat contracture shoulder model

Ochiai, Nobuyasu; Ohtori, Seiji; Kenmoku, Tomonori; Yamazaki, Hironori; Ochiai, Satoko Shiozawa; Saisu, Takashi; Matsuki, Keisuke; Takahashi, Kazuhisa

doi:10.1016/j.jse.2012.02.011

Cited by 15 publications

(23 citation statements)

References 16 publications

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“…The optimal choice of animal and methodology that would be most useful in creating a model for frozen shoulder remains unclear. Because the gross anatomy of the rat shoulder is quite similar to that of humans, four of seven of the aforementioned studies used rats as a frozen shoulder model [15–17, 20]. At present, the development of an animal model for primary frozen shoulder is considered to be an impossibility.…”

Section: Discussionmentioning

confidence: 99%

“…Immobilization has been accepted as common predisposing factor for secondary frozen shoulder. Therefore, most studies have used immobilization for developing a secondary frozen shoulder model and demonstrated that shoulder immobilization can induce adhesion of the joint and capsular contracture [15–17, 20]. There are several different methods that can be used to achieve a shoulder contracture model using immobilization.…”

Section: Discussionmentioning

confidence: 99%

“…There are several different methods that can be used to achieve a shoulder contracture model using immobilization. Molding plaster or extra-articular fixation using a plastic plate or suture material has been used for this purpose [15–17, 20]. In the current study, we chose a rat shoulder contracture model using molding plaster because it was considered a simple and effective secondary frozen shoulder model based on literature reviews and the results of a pilot study.…”

Section: Discussionmentioning

confidence: 99%

“…Although animal frozen shoulder models using mouse, rat, or canine have been developed [14–20], a rat shoulder contracture model using immobilization has been among the most frequently established due to similarities with human anatomy and a high levels of reproducibility [15–17, 20]. However, few studies have been conducted to investigate the serial pathophysiologic changes to the glenohumeral joint following immobilization while developing such animal frozen shoulder models.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a frozen shoulder model using immobilization in rats

Kim

Lee²,

Lho

et al. 2016

J Orthop Surg Res

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BackgroundThe objective of this study was to investigate serial changes for histology of joint capsule and range of motion of the glenohumeral joint after immobilization in rats. We hypothesized that a rat shoulder contracture model using immobilization would be capable of producing effects on the glenohumeral joint similar to those seen in patients with frozen shoulder.MethodsSixty-four Sprague-Dawley rats were randomly divided into one control group (n = 8) and seven immobilization groups (n = 8 per group) that were immobilized with molding plaster for 3 days, or for 1, 2, 3, 4, 5, or 6 weeks. At each time point, eight rats were euthanized for histologic evaluation of the axillary recess and for measurement of the abduction angle.ResultsInfiltration of inflammatory cells was found in the synovial tissue until 2 weeks after immobilization. However, inflammatory cells were diminished and fibrosis was dominantly observed in the synovium and subsynovial tissue 3 weeks after immobilization. From 1 week after immobilization, the abduction angle of all immobilization groups at each time point was significantly lower than that of the control group.ConclusionsOur study demonstrated that a rat frozen shoulder model using immobilization generates the pathophysiologic process of inflammation leading to fibrosis on the glenohumeral joint similar to that seen in patients with frozen shoulder. This model was attained within 3 weeks after immobilization. It may serve as a useful tool to investigate pathogenesis at the molecular level and identify potential target genes that are involved in the development of frozen shoulder.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of a frozen shoulder model using immobilization in rats

Kim

Lee²,

Lho

et al. 2016

J Orthop Surg Res

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“…These observations suggest that understanding the etiology at the molecular level is essential to establish better therapeutic interventions and prophylaxis for frozen shoulder. In accordance with these observations, several studies have investigated changes in the gene expression patterns of joint tissues collected from human patients with frozen shoulder and from animal models (including rat, rabbit, and canine) of joint contracture . These studies have provided important information concerning the pathology of frozen shoulder and the potential target genes for treatment.…”

mentioning

confidence: 92%

Generation and characterization of a novel shoulder contracture mouse model

Oki

Shirasawa

Yoda

et al. 2015

Journal Orthopaedic Research

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Frozen shoulder is a relatively common disorder that leads to severe pain and stiffness in the shoulder joint. Although this disorder is self-limiting in nature, the symptoms often persist for years, resulting in severe disability. Recent studies using human specimens and animal models have shown distinct changes in the gene expression patterns in frozen shoulder tissue, indicating that novel therapeutic intervention could be achieved by controlling the genes that are potentially involved in the development of frozen shoulder. To achieve this goal, it is imperative to develop a reliable animal joint contracture model in which gene expression can be manipulated by gene targeting and transgenic technologies. Here, we describe a novel shoulder contracture mouse model. We found that this model mimics the clinical presentation of human frozen shoulder and recapitulates the changes in the gene expression pattern and the histology of frozen shoulder and joint contracture in humans and other larger animal models. The model is highly reproducible, without any major complications. Therefore, the present model may serve as a useful tool for investigating frozen shoulder etiology and for identifying its potential target genes.

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Efficacy of hyaluronic acid or steroid injections for the treatment of a rat model of rotator cuff injury

Yamaguchi

Ochiai

Sasaki

et al. 2015

Journal Orthopaedic Research

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This study evaluated dorsal root ganglia from C3-C7, analyzed gait, and compared the expression of calcitonin generelated peptide (CGRP) which was a marker of inflammatory pain in a rat rotator cuff tear model in which the supraspinatus and infraspinatus tendons were detached; comparisons were made to a sham group in which only the tendons were exposed. Fluorogold was injected into the glenohumeral joint 21 days after surgery in both groups, and saline, steroids, or hyaluronic acid was injected into the glenohumeral joint in the rotator cuff tear group 26 days after surgery. The proportions of CGRP-immunoreactive neurons were higher and the gait parameters were impaired in the rotator cuff tear group compared to in the sham group. However, the CGRP expression was reduced and the gait was improved with steroid or hyaluronic acid injection compared to saline, suggesting that both hyaluronic acid and steroid injections suppressed of inflammation which thought to be provided pain relief. While there were no significant differences, the suppression of CGRP expression and the improved gait after hyaluronic acid and steroid injections suggested that both methods were effective for rat rotator cuff tear model. Keywords: rotator cuff tear model; pain relief; hyaluronic acid; dorsal root ganglion; CatWalkThe main symptoms of a rotator cuff tear (RCT) are shoulder pain and limited range of motion. Conservative treatment for RCT consists of medication, fomentation, or injections into the subacromial bursa (SAB) or glenohumeral joint (GHJ). In addition, physical rehabilitation has also been used. Although both steroid 1-3 and hyaluronic acid (HA) injections have been commonly used as a conservative treatment for RCT, it remains unknown which method is more effective for pain relief 2,4-6 Moreover, repeated steroid injections may result in local tissue degradation, including tendon tearing and steroid arthropathy. 1 Although the cause of pain in shoulder pathology has not been fully clarified, calcitonin gene-related peptide (CGRP) was found to be highly expressed in both the GHJ and SAB in a rat contracture shoulder model. These results indicate that up-regulation of CGRP might be closely related to the cause of pain in frozen shoulder; 7 the same mechanism of up-regulation of CGRP has also been hypothesized to be related to RCT of inflammatory pain. For both the GHJ and SAB, sensory information is transmitted from free nerve endings through the dorsal root ganglia (DRG) at C3-C7 to the spinal cord and brain. 8 For investigation of inflammation-related pain, evaluation at the level of the DRG is important especially the expression of CGRP.The purpose of the present study was (i) to evaluate DRG neurons innervating the GHJ and SAB using immunohistochemistry for CGRP in a rat RCT model and (ii) to determine whether steroid or HA injection would provide more effective in rotator cuff tear model. MATERIALS AND METHODSAll animal protocols were approved by the Ethics Committee of Chiba University and conducted in accorda...

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Sensory innervation of rat contracture shoulder model

Cited by 15 publications

References 16 publications

Characterization of a frozen shoulder model using immobilization in rats

Characterization of a frozen shoulder model using immobilization in rats

Generation and characterization of a novel shoulder contracture mouse model

Efficacy of hyaluronic acid or steroid injections for the treatment of a rat model of rotator cuff injury

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