Sensory hair cells of a fish ear: evidence of multiple types based on ototoxicity sensitivity

Yan, Hong; Saidel, William M.; Chang, Janet S.; Presson, Joelle C.; Popper, Arthur N.

doi:10.1098/rspb.1991.0099

Cited by 54 publications

(22 citation statements)

References 23 publications

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“…Nevertheless, this study shows that gentamicin led to hair cell bundle damage: fusion of stereocilia and areas without any hair bundles. This type of hair cell bundle alteration has previously been described in the fish and lizard inner ear (Presson and Popper, 1990;Yan et al, 1991;Lombarte et al, 1993;Avallone et al, 2008). The average hair cell density in the inner ear sacculi of the control fish (0.022 ± 0.005 hair cells lm À2 ) was in concordance with the average of 0.025 hair cells lm À2 previously described by Dale (1976) in the same fish species.…”

Section: Discussionsupporting

confidence: 89%

“…All fish were anaesthetized by immersion in sea water containing metacaine (55 mg l À1 ) for 5 min and fitted with coloured floy tags (FLOY TAG Inc., USA) for identification before gentamicin injection. Given the health problems and mortality reported in the literature for intramuscular gentamicin injection (Yan et al, 1991;Lombarte et al, 1993), more fish were used for gentamicin intravenous injection (n = 160) than for intrasaccular injection (n = 75) experiments.…”

Section: Animal Origin and Fish Taggingmentioning

confidence: 99%

“…In contrast, intramuscular injections of gentamicin have led to damage of inner ear hair cells in freshwater fish (Yan et al, 1991;Lombarte et al, 1993). This aminoglycoside antibiotic, however, selectively damaged sensory hair cells in the inner ear lagena and utriculus of the teleost species, oscar (Astronotus ocellatus).…”

Section: Introductionmentioning

confidence: 99%

“…This aminoglycoside antibiotic, however, selectively damaged sensory hair cells in the inner ear lagena and utriculus of the teleost species, oscar (Astronotus ocellatus). No apparent damage to saccular hair cells was observed, even after high-dose gentamicin intramuscular injection (Yan et al, 1991). An alternative route of administration is the injection of aminoglycosides directly into the fish sacculus.…”

Section: Introductionmentioning

confidence: 99%

“…Following intrasaccular aminoglycoside injections, no adverse health effects were reported. After intramuscular injections of gentamicin, health disorders were common in the fish: loss of appetite, erratic swimming behaviour associated with weight gain, bloating of the abdomen and swelling around the eyes, indicative of osmoregulatory problems (Yan et al, 1991;Lombarte et al, 1993). Gentamicin causes renal injury after peritoneal injection in the goldfish, C. auratus (Liu et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

et al. 2008

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a b s t r a c tFish models are increasingly being used for hearing research investigations. Aminoglycoside antibiotics that are used for damaging the inner ear hair cells can have systemic side effects leading to death of study animals. This study aimed to compare two methods: (i) systemic (intravenous) and (ii) local (intrasaccular) gentamicin administration for induction of inner ear hair cell damage in the Atlantic cod, Gadus morhua (L.). Hair cell damage was assessed using scanning electron microscopy; hair cell density, prevalence of immature hair cells and kinocilia length were measured. Gentamicin-treated fish were compared with control and sham fish. Intravenous gentamicin led to dose-dependent mortality caused by nephrotoxicity. The only visible effect after treatment was more immature hair cells and shorter kinocilia, the effect on hair cell density was equivocal. Following intrasaccular gentamicin treatment, fish mortality was negligible, and hair cells were damaged regardless of dose. Here, we observed decreased hair cell density, high prevalence of immature hair cells, and significantly shortened kinocilia. Conclusion: intrasaccular injection is preferable to intravenous injection of gentamicin for the study of ototoxicity in the Atlantic cod.

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Section: Discussionsupporting

confidence: 89%

Section: Animal Origin and Fish Taggingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

et al. 2008

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Hair cell development in vivo and in vitro: Analysis by using a monoclonal antibody specific to hair cells in the chick inner ear

Kondo

Sagara

Hirosawa

et al. 2002

J of Comparative Neurology

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The purpose of this study was to establish a hair cell-specific marker and a convenient explant culture system for developing chick otocysts to facilitate in vivo and in vitro studies focusing on hair cell genesis in the inner ear. To achieve this, a hair cell-specific monoclonal antibody, 2A7, was generated by immunizing chick inner ear tissues to a mouse. Through the use of immunofluorescence and immunoelectron microscopy, it was shown that 2A7 immunoreactivity (2A7-IR) was primarily restricted to the apical region of inner ear hair cells, including stereocilia, kinocilia, apical membrane amongst the extending cilia, and superficial layer of the cuticular plate. Although the 2A7 antibody immunolabeled basically all of the hair cells in the posthatch chick inner ear, two different patterns of 2A7-IR were observed; hair cells located in the striolar region of the utricular macula, which consist of two distinct cell types identifiable on the basis of the type of nerve ending, Type I and II hair cells, showed labeling restricted to the basal end of the hair bundles. On the other hand, hair cells in the extrastriolar region, which are exclusively of Type II, showed labeling extending over virtually the entire length of the bundles. These findings raised the possibility that chick vestibular Type II hair cells, characterized by their bouton-type afferent nerve endings, can be divided into two subpopulations. Analysis of developing inner ear by using the 2A7 antibody revealed that this antibody also recognizes newly differentiated immature hair cells. Thus, the 2A7 antibody is able to recognize both immature and mature hair cells in vivo. The developmental potential of embryonic otocysts in vitro was then assessed by using explant cultures as a model. In this study, conventional otocyst explant cultures were modified by placing the tissues on floating polycarbonate filters on culture media, thereby allowing the easy manipulation of explants. In these cultures, 2A7-positive hair cells were differentiated from dividing precursor cells in vitro on the same schedule as in vivo. Furthermore, it was found that hair cells with both types of 2A7-IR were generated in culture as in vivo, indicating that a maturational process of hair cells also occurred. All these results as presented here suggest that the 2A7 monoclonal antibody as a hair cell-specific marker together with the culture system could be a potential tool in analysis of mechanisms underlying hair cell development.

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Morphology and cell type heterogeneities of the inner ear epithelia in adult and juvenile zebrafish (Danio rerio)

Bang

Sewell

Malicki

2001

J of Comparative Neurology

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Although the zebrafish has become an important model for genetic analysis of the vertebrate auditory system, a comprehensive description of the zebrafish ear has been provided for embryonic and larval development only (Haddon and Lewis [1996] J. Comp. Neurol. 365:113). Here we describe the development of sensory maculae in juvenile fish and the morphology of the adult zebrafish ear. This description was obtained via three-dimensional reconstruction of serial sections and confocal microscopy of immunolabeled preparations and includes the Weberian ossicles and fluid spaces. Phalloidin staining, which labels actin filaments of stereocilia, was used to delineate the sensory epithelia, to visualize the distribution of hair cells, to estimate their density in different areas of the maculae, and to perform hair cell counts. Morphology of ciliary bundles in different regions of the lagena, saccule, utricle, macula neglecta, and cristae was characterized with an anti-acetylated tubulin antibody and by phalloidin staining. We have identified two antibodies characterized by region-specific staining patterns in the inner ear epithelia. Zn-1 antibody staining largely correlates with the presence of short-bundle hair cells in the peripheral regions of sensory epithelia. Zn-4 antibody, on the other hand, labels a zone of epithelial cells surrounding the sensory maculae. These analyses extend previous observations of cell-type heterogeneity in both sensory and nonsensory epithelia of the fish ear.

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Sensory hair cells of a fish ear: evidence of multiple types based on ototoxicity sensitivity

Cited by 54 publications

References 23 publications

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations

Hair cell development in vivo and in vitro: Analysis by using a monoclonal antibody specific to hair cells in the chick inner ear

Morphology and cell type heterogeneities of the inner ear epithelia in adult and juvenile zebrafish (Danio rerio)

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