Sensory evoked fMRI paradigms in awake mice

Chen, Xifan; Tong, Chuanjun; Han, Zhe; Zhang, Kaiwei; Bo, Binshi; Feng, Yanqiu; Liang, Zhifeng

doi:10.1016/j.neuroimage.2019.116242

Cited by 63 publications

(100 citation statements)

References 46 publications

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“…We have successfully developed an awake fMRI protocol by designing a restraint apparatus and habituation protocol. Many attempts have been made to perform awake mouse fMRI to minimize the effects of anesthesia on neural activity and neurovascular coupling (Chen et al, 2020; Desai et al, 2011; Desjardins et al, 2019; Harris et al, 2015; Schlegel et al, 2015; Yoshida et al, 2016). The fundamental requirement for studying awake animals is a restraint apparatus that can minimize head motion and reduce artifacts associated with body motion.…”

Section: Discussionmentioning

confidence: 99%

“…Minimization of head motion can easily be achieved by adopting the head post, but the procedure to attach the head post to the skull often induces image artifacts through residual blood clots and trapped air bubbles. To minimize the translation of body motions to the head, most published studies tried to use a long tube fitted to the animal body, and some tried to secure all animal limbs using surgical tape, with the animal’s incisors secured over a bite bar (Chen et al, 2020; Desai et al, 2011; Desjardins et al, 2019; Han et al, 2019; Harris et al, 2015; Tsurugizawa et al, 2020; Yoshida et al, 2016). All those procedures could induce more stress and artifacts from body movement.…”

Section: Discussionmentioning

confidence: 99%

“…1A). To secure the head post onto the dorsal skull, we adopted the standard aseptic surgical procedures reported in (Chen et al, 2020; Schlegel et al, 2018). Animals were initially anesthetized with ketamine/xylazine anesthesia (100 mg/kg and 10 mg/kg, respectively, intraperitoneal [IP]).…”

Section: Methodsmentioning

confidence: 99%

“…Also, frame-wise displacements (FD) were calculated from fMRI data using the sum of the absolute displacements of the six motion parameters for each animal and assuming that the mouse brain is a sphere with a radius of 5 mm (Belloy et al, 2018; Jung et al, 2019; Power, Barnes, Snyder, Schlaggar, & Petersen, 2012). After the six motion parameter regression was applied to reduce motion artifacts (Chen et al, 2020; Satterthwaite et al, 2013), the time series were normalized by the average of the pre-stimulus baseline volumes.…”

Section: Methodsmentioning

confidence: 99%

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Characteristics of fMRI responses to visual stimulation in anesthetized vs. awake mice

Dinh

Jung

Shim

et al. 2020

Preprint

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The functional characteristics of the mouse visual system have not previously been well explored using fMRI. In this research, we examined 9.4 T BOLD fMRI responses to visual stimuli of varying pulse durations (1:50 ms) and temporal frequencies (1:10 Hz) under ketamine and xylazine anesthesia and compared fMRI responses of anesthetized and awake mice. Under anesthesia, significant positive BOLD responses were detected bilaterally in the major structures of the visual pathways, including the dorsal lateral geniculate nuclei, superior colliculus, the lateral posterior nucleus of the thalamus, primary visual area, and higher-order visual area. BOLD responses increased slightly with pulse duration, were maximal at 3, 5 Hz stimulation, and significantly decreased at 10 Hz, which were all consistent with previous neurophysiological findings. When the mice were awake, the BOLD fMRI response was faster in all active regions and stronger in the subcortical areas compared with the anesthesia condition. In the V1, the BOLD response was biphasic for 5 Hz stimulation and negative for 10 Hz stimulation under wakefulness, whereas prolonged positive BOLD responses were observed at both frequencies under anesthesia. Unexpected activation was detected in the extrastriate postrhinal area and nonvisual subiculum complex under anesthesia, but not under wakefulness. Widespread positive BOLD activity under anesthesia likely results from the disinhibition and sensitization of excitatory neurons induced by ketamine. Overall, fMRI can be a viable tool for mapping brain-wide functional networks.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Characteristics of fMRI responses to visual stimulation in anesthetized vs. awake mice

Dinh

Jung

Shim

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…However, Wavelet Despiking has not been systematically assessed in task-related fMRI, where the frequency spectrum may be more heterogeneous than in resting-state fMRI. Additionally, in rodents, the hemodynamic response function (HRF) is faster than in primates (Chen et al, 2020, Lebhardt et al, 2016, Schlegel et al, 2015) and therefore has a weaker lowpass filtering effect, potentially allowing true neuronally-based BOLD signal to contain higher frequencies. In line with this, we found Wavelet Despiking to introduce implausible temporal “smearing” such that activations started slightly before the trial.…”

Section: Methodsmentioning

confidence: 99%

Hierarchical cross-scale analysis identifies parallel ventral striatal networks coding for dynamic and stabilized olfactory reward predictions

Winkelmeier

Filosa

Scheller

et al. 2021

Preprint

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The unbiased identification of brain circuits responsible for behavior and their local cellular computations is a challenge for neuroscience. We establish here a hierarchical cross-scale approach from behavioral modeling and fMRI in task-performing mice to cellular network dynamics to identify how reward predictions are represented in the forebrain upon olfactory conditioning. fMRI identified functional segregation in reward prediction and error computations among olfactory cortices and subcortical circuits. Among them, the olfactory tubercle contributed both to dynamic reward predictions and prediction error. In this region, cellular recordings revealed two parallel neuronal populations for prediction coding. One population produced stabilized predictions as distributed stimulus-bound transient network activity; the other evolved during anticipatory waiting and fully reflected predicted value in single-units, dynamically integrating the recent cue-specific history of uncertain outcomes. Thus, the cross-scale approach revealed regional functional differentiation among the distributed forebrain circuits with a limbic hotspot for multiple non-redundant reward prediction coding.

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Simultaneous Functional Magnetic Resonance and Optoacoustic Imaging of Brain‐Wide Sensory Responses in Mice

et al. 2022

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Functional magnetic resonance imaging (fMRI) has massively contributed to the understanding of mammalian brain function. However, the origin and interpretation of the blood oxygen level‐dependent (BOLD) signals retrieved by fMRI remain highly disputed. This article reports on the development of a fully hybridized system enabling concurrent functional magnetic resonance optoacoustic tomography (MROT) measurements of stimulus‐evoked brain‐wide sensory responses in mice. The highly complementary angiographic and soft tissue contrasts of both modalities along with simultaneous multi‐parametric readings of stimulus‐evoked hemodynamic responses are leveraged in order to establish unequivocal links between the various counteracting physiological and metabolic processes in the brain. The results indicate that the BOLD signals are highly correlated, both spatially and temporally, with the total hemoglobin readings resolved with volumetric multi‐spectral optoacoustic tomography. Furthermore, the differential oxygenated and deoxygenated hemoglobin optoacoustic readings exhibit superior sensitivity as compared to the BOLD signals when detecting stimulus‐evoked hemodynamic responses. The fully hybridized MROT approach greatly expands the neuroimaging toolset to comprehensively study neurovascular and neurometabolic coupling mechanisms and related diseases.

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Sensory evoked fMRI paradigms in awake mice

Cited by 63 publications

References 46 publications

Characteristics of fMRI responses to visual stimulation in anesthetized vs. awake mice

Characteristics of fMRI responses to visual stimulation in anesthetized vs. awake mice

Hierarchical cross-scale analysis identifies parallel ventral striatal networks coding for dynamic and stabilized olfactory reward predictions

Simultaneous Functional Magnetic Resonance and Optoacoustic Imaging of Brain‐Wide Sensory Responses in Mice

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