Sensory and vascular changes in a rat monoarthritis model: prophylactic and therapeutic effects of meloxicam

Hashmi, Javeria A.; Yashpal, Kiran; Holdsworth, David W.; Henry, James L.

doi:10.1007/s00011-010-0179-3

Cited by 15 publications

(9 citation statements)

References 56 publications

(70 reference statements)

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“…Our results confirmed that the complete Freund adjuvant model reproduced allodynia and hyperalgesia, as commonly described in patients (28). Pain behavior was dose-dependently reduced by meloxicam as previously shown in inflammatory pain models (21,(29)(30). Signs of inflammation were evidenced as paw circumference (i.e., edema) increase in Moars from day 3 to day 28.…”

Section: Discussionsupporting

confidence: 90%

^99mTc-NTP 15-5 Imaging for Cartilage Involvement in Experimental Rheumatoid Arthritis: Comparison with Routinely Used Molecular Imaging Methods and Sensitivity to Chronic Nonsteroidal Antiinflammatory Drug Treatment

et al. 2015

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This study determined, using the intraarticular complete Freund adjuvant arthritis mice model, whether the radiotracer 99m Tc-N-(triethylammonium)-3-propyl- [15]ane-N5 ( 99m Tc-NTP 15-5) targeting proteoglycans has a pathophysiologic validity for in vivo imaging of rheumatoid arthritis (RA) and its response to chronic nonsteroidal antiinflammatory drugs. Methods: We investigated the time course of cartilage remodeling by 99m Tc-NTP 15-5 scintigraphy, bone damages by 99m Tc-hydroxymethylene diphosphonate imaging, inflammation by 18 F-FDG PET, and joint proteoglycan content and pain behavior in animals, without and with meloxicam treatment. Paw circumference, thermal pain behavior, and histology as well as proteoglycan content of the whole joint were determined. Results: 99m Tc-NTP 15-5 showed specific tracer accumulation within RA joints, with a significant increase in scintigraphic ratio observed in RA versus shams from day 3 to day 28. 18 F-FDG evidenced uptake in RA joints from day 15 to day 29. Animals treated with meloxicam (5 mg/kg) exhibited a dose-dependent decrease in both 99m Tc-NTP 15-5 and 18 F-FDG uptake ratios versus saline-treated animals. 99m Tc-hydroxymethylene diphosphonate bone scans were only positive at day 14 in RA versus shams, with a significant effect of meloxicam. An increase in proteoglycans of RA joint and thermal pain behavior were observed and were dose-dependently reduced by meloxicam. Conclusion: These experimental results bring data in favor of the 99m Tc-NTP 15-5 radiotracer for assessing, in vivo, cartilage remodeling in RA that could be used to monitor therapy.

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Section: Discussionsupporting

confidence: 90%

^99mTc-NTP 15-5 Imaging for Cartilage Involvement in Experimental Rheumatoid Arthritis: Comparison with Routinely Used Molecular Imaging Methods and Sensitivity to Chronic Nonsteroidal Antiinflammatory Drug Treatment

et al. 2015

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“…However, the formation of granulation tissue within the synovial subintimal region resulted in hypertrophic synovial tissue expanded to affect several other joints in the ankle. In this study and a previous study [ 30 ], we used a CFA volume of 20 μL to induce TTJ monoarthritis, which is a smaller volume, compared to many other studies [ 40 , 63 – 66 ]. Even with a volume of 20 μl, unintended massive inflammation affected surrounding tissue in the ankle.…”

Section: Discussionmentioning

confidence: 99%

Effects of buprenorphine on model development in an adjuvant-induced monoarthritis rat model

et al. 2022

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Complete Freund’s adjuvant (CFA)-induced arthritis in rats is a common animal model for studying chronic inflammatory pain. However, modelling of the disease is associated with unnecessary pain and impaired animal wellbeing, particularly in the immediate post-induction phase. Few attempts have been made to counteract these adverse effects with analgesics. The present study investigated the effect of buprenorphine on animal welfare, pain-related behaviour and model-specific parameters during the disease progression in a rat model of CFA-induced monoarthritis. The aim was to reduce or eliminate unnecessary pain in this model, in order to improve animal welfare and to avoid suffering, without compromising the quality of the model. Twenty-four male Sprague Dawley rats were injected with 20 μl of CFA into the left tibio-tarsal joint to induce monoarthritis. Rats were treated with either buprenorphine or carprofen for 15 days during the disease development, and were compared to a saline-treated CFA-injected group or a negative control group. Measurements of welfare, pain-related behaviour and clinical model-specific parameters were collected. The study was terminated after 3 weeks, ending with a histopathologic analysis. Regardless of treatment, CFA-injected rats displayed mechanical hyperalgesia and developed severe histopathological changes associated with arthritis. However, no severe effects on general welfare were found at any time. Buprenorphine treatment reduced facial pain expression scores, improved mobility, stance and lameness scores and it did not supress the CFA-induced ankle swelling, contrary to carprofen. Although buprenorphine failed to demonstrate a robust analgesic effect on the mechanical hyperalgesia in this study, it did not interfere with the development of the intended pathology.

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“…Interestingly, joint immobilization leads to not only contracture, but also joint inflammatory conditions characterized by synovitis [13] and an upregulation of inflammation-related genes [14]. In addition, arthritis-induced contracture is partially prevented and improved by anti-inflammatory drugs [15–17]. As such, anti-inflammatory treatment may prevent immobilization-induced contracture as well as arthritis-induced contracture.…”

Section: Introductionmentioning

confidence: 99%

Cyclooxygenase-2 inhibitor celecoxib attenuates joint contracture following immobilization in rat knees

Ozawa

Kaneguchi

Tanaka

et al. 2016

BMC Musculoskelet Disord

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BackgroundThe aim of this study is to clarify the following two points: First, whether a cyclooxygenase-2 mediated pathway is involved in the formation of immobilization-induced joint contracture and, second, the effectiveness of oral administration of non-steroidal anti-inflammatory drug celecoxib (CBX) for the prevention of myogenic and arthrogenic contracture following immobilization in a rat model.MethodsThirty male rats were randomly divided into three groups: immobilization (Im), Im + CBX, and control (n = 10 each). External fixation immobilized the right knee joint of Im and Im + CBX groups in flexion for 3 weeks. 50 mg/kg of CBX was administrated daily to the Im + CBX group during this period. The passive range of motion (ROM) of knee joints was measured before and after transection of knee flexor muscles and myogenic and arthrogenic ROM restrictions were calculated. The semitendinosus muscles and knee joints were investigated histologically to elucidate factors responsible for contracture.ResultsMyogenic ROM restrictions were exhibited both in Im and Im + CBX groups (44 ± 5 and 36 ± 8 °, respectively), but restrictions significantly decreased in the Im + CBX group compared to the Im group. Significant reductions of the muscle length ratios (Rt/Lt) and sarcomere number ratios (Rt/Lt) in knee flexor semitendinosus muscle, which are responsible for myogenic contracture, were also seen both in Im group (92 ± 5 and 92 ± 4 %, respectively) and Im + CBX group (97 ± 3 and 97 ± 3 %, respectively), but were inhibited by CBX administration (P < 0.05). Im and Im + CBX groups exhibited arthrogenic ROM restrictions with no significant differences (82 ± 3 and 83 ± 5 °, respectively). Posterior synovial length shortening and pathological changes (hemorrhage in joint cavities and capsule edema) in the knee joints were comparable between Im and Im + CBX groups.ConclusionsOral administration of celecoxib partially reduced myogenic ROM restriction concomitantly with knee flexor muscle shortening following immobilization. These results imply that inflammation and nociception are involved in myogenic contracture formation independently of joint immobilization, and that CBX is effective in preventing joint contracture following immobilization in rats.

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Sensory and vascular changes in a rat monoarthritis model: prophylactic and therapeutic effects of meloxicam

Abstract: The present study provides a unique comparison of prophylactic versus therapeutic effects of meloxicam in the CFA-induced model of monoarthritis.

Cited by 15 publications

References 56 publications

^99mTc-NTP 15-5 Imaging for Cartilage Involvement in Experimental Rheumatoid Arthritis: Comparison with Routinely Used Molecular Imaging Methods and Sensitivity to Chronic Nonsteroidal Antiinflammatory Drug Treatment

^99mTc-NTP 15-5 Imaging for Cartilage Involvement in Experimental Rheumatoid Arthritis: Comparison with Routinely Used Molecular Imaging Methods and Sensitivity to Chronic Nonsteroidal Antiinflammatory Drug Treatment

Effects of buprenorphine on model development in an adjuvant-induced monoarthritis rat model

Cyclooxygenase-2 inhibitor celecoxib attenuates joint contracture following immobilization in rat knees

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