Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin

Lai, Chih Ho; Chang, Chia Shuo; Liu, Hsin Ho; Tsai, Yuh Shyan; Hsu, Feng-Ming; Yu, Yung Luen; Lai, Cheng Kuo; Gandee, Leah; Pong, Rey-Chen; Hsu, Heng Wei; Yu, Lan; Saha, Debabrata; Hsieh, Jer Tsong

doi:10.18632/oncotarget.2133

Cited by 24 publications

(28 citation statements)

References 52 publications

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“…PC3-KD cells were maintained in RPMI1640 supplemented with 5% FBS. The cells were cultured in a humidified atmosphere containing 5% CO 2 [27]. …”

Section: Methodsmentioning

confidence: 99%

“…LAPC4-KD cells and nude mice were irradiated at room temperature in ambient air using the Faxitron RX-650 irradiator (Faxitron X-ray, Wheeling, IL) at the indicated doses described in each experiment [27]. …”

Section: Methodsmentioning

confidence: 99%

“…The mice were treated in accordance with the Animal Care and Use Guidelines for Chang Gung University. A suspension of LAPC4 shDAB2IP (2 × 10 6 ) cells mixed with 50% Matrigel (BD Biosciences, Bedford, MA) in 0.1 ml was injected subcutaneously into the right posterior flanks of the mice as described previously [27]. Mice were divided into four groups (6 animals/group), including untreated control, radiation alone, combined treatment of resveratrol (5 mg/kg) or peanut stem extracts (250 mg/kg) with irradiation.…”

Section: Methodsmentioning

confidence: 99%

“…Tissue specimens from mice were formalin-fixed then subjected to IHC staining as described previously [27,34]. Briefly, the tissue sections were de-waxed and rehydrated.…”

Section: Methodsmentioning

confidence: 99%

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Sensitization of Radioresistant Prostate Cancer Cells by Resveratrol Isolated from Arachis hypogaea Stems

et al. 2017

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Resveratrol (RV, 3,4ʹ,5-trihydroxystilbene) is naturally produced by a wide variety of plants including grapes and peanuts (Arachis hypogaea). However, the yield of RV from peanut stem and its potential radiosensitizing effects in prostate cancer (PCa) have not been well investigated. In this study, we characterized RV in peanut stem extract (PSE) for the first time and showed that both RV and PSE dose-dependently induced cell death in DOC-2/DAB2 interactive protein (DAB2IP)-deficient PCa cells with the radioresistant phenotype. Furthermore, the combination of radiation with either RV or PSE induced the death of radioresistant PCa cells through delayed repair of radiation-induced DNA double-strand break (DSB) and prolonged G2/M arrest, which induced apoptosis. The administration of RV and PSE effectively enhanced radiation therapy in the shDAB2IP PCa xenograft mouse model. These results demonstrate the promising synergistic effect of RV and PSE combined with radiation in the treatment of radioresistant PCa.

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“…PC3-KD cells were maintained in RPMI1640 supplemented with 5% FBS. The cells were cultured in a humidified atmosphere containing 5% CO 2 [27]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Tissue specimens from mice were formalin-fixed then subjected to IHC staining as described previously [27,34]. Briefly, the tissue sections were de-waxed and rehydrated.…”

Section: Methodsmentioning

confidence: 99%

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Sensitization of Radioresistant Prostate Cancer Cells by Resveratrol Isolated from Arachis hypogaea Stems

et al. 2017

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“…The lentiviral vectors were constructed using standard molecular cloning procedures as described previously (Lai et al, 2014). Sequence for negative control shRNA (TRCN0000072224) was CGCGATCGTAATCACCCGAGT.…”

Section: Lentiviral Vector Production and Short Hairpin Rna Transfementioning

confidence: 99%

Helicobacter pyloricholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages

Lai

Huang

Cheng

et al. 2018

Cellular Microbiology

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Cholesterol-α-glucosyltransferase (CGT) encoded by the type 1 capsular polysaccharide biosynthesis protein J (capJ) gene of Helicobacter pylori converts cellular cholesterol into cholesteryl glucosides. H. pylori infection induces autophagy that may increase bacterial survival in epithelial cells. However, the role of H. pylori CGT that exploits lipid rafts in interfering with autophagy for bacterial survival in macrophages has not been investigated. Here, we show that wild-type H. pylori carrying CGT modulates cholesterol to trigger autophagy and restrain autophagosome fusion with lysosomes, permitting a significantly higher bacterial burden in macrophages than that in a capJ-knockout (∆CapJ) mutant. Knockdown of autophagy-related protein 12 impairs autophagosome maturation and decreases the survival of internalised H. pylori in macrophages. These results demonstrate that CGT plays a crucial role in the manipulation of the autophagy process to impair macrophage clearance of H. pylori.

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Pretreatment biopsy analysis of DAB2IP identifies subpopulation of high‐risk prostate cancer patients with worse survival following radiation therapy

et al. 2015

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Decreased expression of tumor suppressor DAB2IP is linked to aggressive cancer and radiation resistance in several malignancies, but clinical survival data is largely unknown. We hypothesized that pretreatment DAB2IP reduction would predict worse prostate cancer‐specific survival (PCSS). Immunohistochemistry of pretreatment biopsies was scored by an expert genitourinary pathologist. Other endpoints analyzed include freedom from biochemical failure (FFBF), castration resistance‐free survival (CRFS), and distant metastasis‐free survival (DMFS). Seventy‐nine patients with NCCN‐defined high‐risk prostate cancer treated with radiotherapy from 2005 to 2012 at our institution were evaluated. Twenty‐eight percent (22/79) of pretreatment biopsies revealed DAB2IP‐reduction. The median follow up times were 4.8 years and 5.3 years for patients in the DAB2IP‐reduced group and DAB2IP‐retained group, respectively. Patients with reduced DAB2IP demonstrated worse outcome compared to patients retaining DAB2IP, including FFBF (4‐year: 34 vs. 92%; P < 0.0001), CRFS (4‐year: 58 vs. 96%; P = 0.0039), DMFS (4‐year: 58 vs. 100%; P = 0.0006), and PCSS (5‐year: 83 vs. 100%; P = 0.0102). Univariate analysis showed T stage, N stage, and Gleason score were statistically significant variables. Pretreatment tumor DAB2IP status remained significant in multivariable analyses. This study suggests that about one‐fourth of men with high‐risk prostate cancer have decreased tumor expression of DAB2IP. This subpopulation with reduced DAB2IP has a suboptimal response and worse malignancy‐specific survival following radiation therapy and androgen deprivation. DAB2IP loss may be a genetic explanation for the observed differences in aggressive tumor characteristics and radiation resistance. Further study into improving treatment response and survival in this subpopulation is warranted.

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Sensitization of radio-resistant prostate cancer cells with a unique cytolethal distending toxin

Cited by 24 publications

References 52 publications

Sensitization of Radioresistant Prostate Cancer Cells by Resveratrol Isolated from Arachis hypogaea Stems

Sensitization of Radioresistant Prostate Cancer Cells by Resveratrol Isolated from Arachis hypogaea Stems

Helicobacter pyloricholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages

Pretreatment biopsy analysis of DAB2IP identifies subpopulation of high‐risk prostate cancer patients with worse survival following radiation therapy

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