Sensitization of GSH synthesis by curcumin curtails acrolein-induced alveolar epithelial apoptosis via Keap1 cysteine conjugation: A randomized controlled trial and experimental animal model of pneumonitis

Jo, Eun Hee; Moon, Ji Eun; Chang, Moon Han; Lim, Ye Jin; Park, Jung Hyun; Lee, Suk Hee; Cho, Young-Rae; Cho, Art E.; Pack, Seung Pil; Kim, Hyeon-Wee; Crowley, Liana; Le, Brandy; Aykin-Burns, Nūkhet; Chen, Yinfeng; Zhong, Yihang; Zhao, Jiangchao; Liu, Ying; Cha, Hanvit; Pan, Jeong Hoon; Kim, Jae Kyeom; Lee, Jin Hyup

doi:10.1016/j.jare.2022.06.013

Cited by 6 publications

(2 citation statements)

References 76 publications

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“…Activation of the antioxidant defence through the Nrf2 pathway observed by the luciferase reporter assay might not be only due to the increase in ROS production from the activation of NADPH oxidase. Acrolein as an electrophile can induce the activation of Nrf2 independently of NADPH oxidase, by depletion of GSH [ 32 ], by limiting the enzymatic activity of thioredoxin, peroxiredoxins and other Nrf2 transcription targets, through adduct formation [ 70 ] and by potentially reacting with KEAP1 cysteine residues, promoting Nrf2-KEAP1 dissociation [ 31 ] [ 4 ]. This would imply that Nrf2-dependent antioxidant defence is activated immediately following acrolein exposure and further upon ROS production by the activated NADPH oxidase complex [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

The role of acrolein for E-cigarette vapour condensate mediated activation of NADPH oxidase in cultured endothelial cells and macrophages

Kuntic

Oelze

et al. 2023

Pflugers Arch - Eur J Physiol

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Electronic cigarettes (E-cigarettes) have recently become a popular alternative to traditional tobacco cigarettes. Despite being marketed as a healthier alternative, increasing evidence shows that E-cigarette vapour could cause adverse health effects. It has been postulated that degradation products of E-cigarette liquid, mainly reactive aldehydes, are responsible for those effects. Previously, we have demonstrated that E-cigarette vapour exposure causes oxidative stress, inflammation, apoptosis, endothelial dysfunction and hypertension by activating NADPH oxidase in a mouse model. To better understand oxidative stress mechanisms, we have exposed cultured endothelial cells and macrophages to condensed E-cigarette vapour (E-cigarette condensate) and acrolein. In both endothelial cells (EA.hy 926) and macrophages (RAW 264.7), we have observed that E-cigarette condensate incubation causes cell death. Since recent studies have shown that among toxic aldehydes found in E-cigarette vapour, acrolein plays a prominent role, we have incubated the same cell lines with increasing concentrations of acrolein. Upon incubation with acrolein, a translocation of Rac1 to the plasma membrane has been observed, accompanied by an increase in oxidative stress. Whereas reactive oxygen species (ROS) formation by acrolein in cultured endothelial cells was mainly intracellular, the release of ROS in cultured macrophages was both intra- and extracellular. Our data also demonstrate that acrolein activates the nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant pathway and, in general, could mediate E-cigarette vapour-induced oxidative stress and cell death. More mechanistic insight is needed to clarify the toxicity associated with E-cigarette consumption and the possible adverse effects on human health.

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Section: Discussionmentioning

confidence: 99%

The role of acrolein for E-cigarette vapour condensate mediated activation of NADPH oxidase in cultured endothelial cells and macrophages

Kuntic

Oelze

et al. 2023

Pflugers Arch - Eur J Physiol

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“…The accumulation of GSSGs may occur if pollutants or their byproducts directly impede GR activity. Prolonged exposure to pollutants can lead to the depletion of the cellular GSH pool, as GSH is utilized in the process of neutralizing ROS [80,81]. This depletion ultimately results in a diminished GSH/GSSG ratio.…”

Section: Discussionmentioning

confidence: 99%

Seawater Accelerated the Aging of Polystyrene and Enhanced Its Toxic Effects on Caenorhabditis elegans

Zhou,

Wu,

Liu

et al. 2023

IJMS

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Microplastics (MPs) are emerging pollutants and pose a significant threat to marine ecosystems. Although previous studies have documented the mechanisms and toxic effects of aging MPs in various environments, the impact of the marine environment on MPs remains unclear. In the present study, the aging process of polystyrene (PS) in seawater was simulated and the changes in its physicochemical properties were investigated. Our results showed that the surface of the PS eroded in the seawater, which was accompanied by the release of aged MPs with a smaller size. In situ optical photothermal infrared microspectroscopy revealed that the mechanism of PS aging was related to the opening of the carbonyl group and breaking of the bond between carbon and benzene removal. To verify the toxic effects of aged PS, Caenorhabditis elegans was exposed to PS. Aged PS resulted in a greater reduction in locomotion, vitality, and reproduction than virgin PS. Mechanistically, aged PS led to oxidative stress, high glutathione s-transferase activity, and high total glutathione in worms. Together, our findings provided novel information regarding the accelerated aging of PS in seawater and the increased toxicity of aged PS, which could improve our understanding of MPs’ ecotoxicity in the marine environment.

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Association between acrolein exposure and respiratory hazards: A systematic review and meta-analysis

Liu

Lou

Zhang

et al. 2023

Atmospheric Pollution Research

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Sensitization of GSH synthesis by curcumin curtails acrolein-induced alveolar epithelial apoptosis via Keap1 cysteine conjugation: A randomized controlled trial and experimental animal model of pneumonitis

Cited by 6 publications

References 76 publications

The role of acrolein for E-cigarette vapour condensate mediated activation of NADPH oxidase in cultured endothelial cells and macrophages

The role of acrolein for E-cigarette vapour condensate mediated activation of NADPH oxidase in cultured endothelial cells and macrophages

Seawater Accelerated the Aging of Polystyrene and Enhanced Its Toxic Effects on Caenorhabditis elegans

Association between acrolein exposure and respiratory hazards: A systematic review and meta-analysis

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