Sensitivity to salinization and acclimation potential of amphibian (Pelophylax perezi) and fish (Lepomis gibbosus) models

Venâncio, Cátia; Castro, Bruno B.; Ribeiro, Rui; Antunes, Sara C.; Lopes, Isabel

doi:10.1016/j.ecoenv.2019.01.099

Cited by 24 publications

(19 citation statements)

References 47 publications

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“…Freshwater ecosystems have been degraded by increases in salinity [1], and many organisms inhabiting in freshwater habitats are physiologically affected by this salinization [2]. In particular, amphibians are more sensitive to changes in salinity than other taxa [3] because they have permeable skin and a complex life history [4]. Amphibians are highly threatened, experiencing the highest extinction rates in vertebrates (ca.…”

Section: Introductionmentioning

confidence: 99%

Physiological Response of Pelophylax nigromaculatus Adults to Salinity Exposure

Park

2020

Animals

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Many freshwater ecosystems are becoming more saline, and amphibians, which have permeable skin, are sensitive to this change. We studied the physiological responses to high salinity and recovery from saline exposure in adult frogs (Pelophylax nigromaculatus). Frogs that experienced severe salinity were exposed to saline conditions for 6 days, while those in the moderate group were exposed to saline conditions for 40 days, followed by a recovery period in freshwater for 20 days. Our data showed that during exposure to saline conditions of severe and moderate groups, serum electrolytes increased, protein concentrations decreased, and creatinine, an indicator of renal function, sharply increased. However, renal tissue sampled after exposure did not show renal dysfunction. In addition, serum components that changed during exposure to salinity returned to their initial values during the recovery period. Thus, adult anurans can be resilient, to some extent, to saline conditions in habitats that experience either rapid or slow salinity changes.

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Section: Introductionmentioning

confidence: 99%

Physiological Response of Pelophylax nigromaculatus Adults to Salinity Exposure

Park

2020

Animals

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“…But, a large amount of them have not been utilized by fisheries, and the research on fish culture in saline‐alkali waters needs further breakthrough. Research on salt and alkali tolerance of fish has been carried out relatively late, which most of the resistance studies have performed using conventional physiological and biochemical studies [31, 32]. Although high salinity in water have different physical and chemical effects and toxic effects on fish, Barbus capito can still grow and reproduce normally in a certain concentration of saline‐alkali environment.…”

Section: Discussionmentioning

confidence: 99%

“…But, a large amount of them have not been utilized by fisheries, and the research on fish culture in saline-alkali waters needs further breakthrough. Research on salt and alkali tolerance of fish has been carried out relatively late, which most of the resistance studies have performed using conventional physiological and biochemical studies [31,32]. excellent salt-and-alkali-resistant has become a better experimental materials for research on fish germplasm resources and salt and alkali tolerance mechanisms [33].…”

Section: Discussionmentioning

confidence: 99%

High‐throughput metabolomics method based on liquid chromatography‐mass spectrometry: Insights into the underlying mechanisms of salinity–alkalinity exposure‐induced metabolites changes in Barbus capito

Sun

Han

Yao

et al. 2020

J of Separation Science

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It is critical to investigate the adaptive development and the physiological mechanism of fish in external stimulation. In this study, the response of Barbus capito to salinity–alkalinity exposure was explored by high‐throughput nontargeted and liquid chromatography–mass spectrometry‐based metabolomics to investigate metabolic biomarker and pathway changes. Meanwhile, the biochemical indexes of Barbus capito were measured to discover the chronic impairment response to salinity–alkalinity exposures. A total of 29 tissue metabolites were determined to deciphering the endogenous metabolic changes of fishes during the different concentration salinity–alkalinity exposures environment, which were mainly involved in the key metabolism including the phenylalanine, tyrosine, and tryptophan biosynthesis, arachidonic acid metabolism, pyruvate metabolism, citrate cycle, and glycerophospholipid metabolism. Finally, we found the amino acid metabolism as key target was associated with the endogenous metabolites and metabolic pathways of Barbus capito to salinity–alkalinity exposures. In conclusion, metabolomics is a potentially powerful tool to reveal the mechanism information of fish in various exposure environments.

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“…Although a subset of amphibians is tolerant of saline conditions (Hopkins and Brodie, 2015), many are considered vulnerable to increased salinization because they have narrow osmotic ranges (Chinathamby et al, 2006;Sanzo and Hecnar, 2006;Brady, 2013), possess highly permeable skin (Pough, 2007), and rely on freshwater habitats for reproduction, development, and dwelling (Vences and Köhler, 2008). While even modest increases in salt can be detrimental (Venâncio et al, 2019), concentrations can become especially high in shallow and/or temporary habitats (e.g., vernal 4 pools), which many amphibians require to complete their lifecycle (Semlitsch and Skelly, 2007).…”

Section: Salt Pollution: a Widespread Problem For Freshwater Organismsmentioning

confidence: 99%

“…Elevated salinity in freshwater habitats spurs a suite of negative effects across biological levels, from genetic to ecosystem (Hintz et al, 2017;Gibbons et al, 2018;Merrick and Searle, 2019;Venâncio et al, 2019). For many freshwater taxa, salt pollution causes numerous sublethal effects, including changes in foraging and anti-predator behavior (Hall et al, 2017); changes in life history traits (Faulkner et al, 2019); and increased malformations (Brady, 2013;Hopkins et al, 2013;Alam et al, 2020).…”

Section: Salt Pollution: a Widespread Problem For Freshwater Organismsmentioning

confidence: 99%

Road salt compromises functional morphology of larval gills in populations of an amphibian

Szeligowski

Scanley

Broadbridge

et al. 2020

Preprint

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Throughout much of the world, winter deicing practices have led to secondary salinization of freshwater habitats, where numerous taxa are vulnerable to elevated salinity. Many amphibians are of particular concern because of their permeable skin and reliance on small ponds and pools, where salinity levels can be high. The early life-history stages of amphibians that develop in these habitats are especially sensitive to salt exposure. Larvae developing in salt-polluted environments must osmoregulate through ion exchange in gills. While salt-induced changes to the physiology of ion exchange in amphibian gills is generally understood, functionally relevant changes in gill morphology remain poorly described. Yet the structure of gills should be an important component affecting their ionoregulatory capacity, for instance in terms available surface area. Larval amphibian gills also play critical roles in gas exchange and foraging. Thus, changes in gill morphology due to salt pollution potentially affect not only osmoregulation, but also respiration and feeding. Here, we used a chronic exposure experiment to quantify the effect of salinity on larval gill morphology in populations of the wood frog (Rana sylvatica). We measured a suite of morphological traits on gill tufts, where ionoregulation and gas exchange occur, and on gill filters, which are used in feeding. Larvae raised in high salinity conditions had gill tufts with lower surface area to volume ratio, while epithelial cells on these tufts were less circular but occurred at higher densities. Gill filters showed increased spacing, which can potentially reduce their efficiency in filtering food particles. Together, these changes seem likely to diminish the ionoregulatory and respiratory capacity of gill tufts, and compromise feeding functionality of gill filters. Thus, a singular change in the aquatic environment from a widespread pollutant has the potential to generate a suite of consequences via changes in gill morphology. Critically, this suite of negative effects is likely most detrimental in salinized environments, where ionoregulatory demands are higher, which in turn should increase respiratory demands along with energy acquisition demands through foraging.Summary StatementChronic road salt exposure alters the functional morphology of gills in larval amphibians, potentially compromising osmoregulation, feeding, and respiration.

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Sensitivity to salinization and acclimation potential of amphibian (Pelophylax perezi) and fish (Lepomis gibbosus) models

Cited by 24 publications

References 47 publications

Physiological Response of Pelophylax nigromaculatus Adults to Salinity Exposure

Physiological Response of Pelophylax nigromaculatus Adults to Salinity Exposure

High‐throughput metabolomics method based on liquid chromatography‐mass spectrometry: Insights into the underlying mechanisms of salinity–alkalinity exposure‐induced metabolites changes in Barbus capito

Road salt compromises functional morphology of larval gills in populations of an amphibian

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