Sensitivity to dynorphin-(1–13) of the presynaptic inhibitory opiate receptors of the guinea-pig heart

Ledda, F.; Mantelli, Laura; Corti, Vittorio

doi:10.1016/0014-2999(85)90013-5

Cited by 36 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are in agreement with previous reports showing preganglionic modulation of mainly cardiac parasympathetic neurotransmitter release (Montel and Starke, 1973;Ledda et al, 1984Ledda et al, , 1985Musha et al, 1989;Adams and Trequattrini, 1998). Because it was previously reported that the autonomic innervations of the rat heart is immature at birth (Smith et al, 1981;Malfatto Slaviková et al, 1993;Slaviková, 1997;Horackova et al, 2000), we also investigated the co-localization of DOR with VAChT neurons in P1 and P7 rats.…”

Section: D-opioid Receptors In the Developing Rat Heartsupporting

confidence: 95%

Developmental expression of δ‐opioid receptors during maturation of the parasympathetic, sympathetic, and sensory innervations of the neonatal heart: Early targets for opioid regulation of autonomic control

Mousa

Shaqura

Schäper

et al. 2011

J of Comparative Neurology

View full text Add to dashboard Cite

Evidence is accumulating regarding the local opioid regulation of heart function. However, the exact anatomical location of δ-opioid receptors (DORs) and expression during maturation of the autonomic and sensory innervations of the neonatal heart is unknown. Therefore, we aimed to characterize target sites for opioids in neonatal rat heart intracardiac ganglia at postnatal day (P)1, P7 and adulthood (P56-P84). Rat heart atria were subjected to reverse-transcriptase polymerase chain reaction, Western blot, radioligand binding, and immunofluorescence confocal analysis of DORs with the neuronal markers vesicular acetylcholine transporter (VAChT), tyrosine hydroxylase (TH), calcitonin gene-related peptide (CGRP), and substance P (SP). Our results demonstrated DOR mRNA, protein, and binding sites that gradually increased from P1 toward adulthood. Immunofluorescence confocal microscopy showed DOR co-localized with VAChT in large-diameter principal neurons, TH-immunoreactive (IR) small intensely fluorescent (SIF) catecholaminergic cells, and CGRP- or SP-IR afferent nerve terminals arborizing within intracardiac ganglia and atrial myocardium. Co-expression of DOR with VAChT-IR neurons was observed from the first day of birth (P1). In contrast, DORs on TH-IR SIF cells or CGRP-IR fibers were not observed in intracardiac ganglia of P1, but rather in P7 rats. The density of nerve fibers in atrial myocardium co-expressing DORs with different neuronal markers increased from neonatal age toward adulthood. In summary, the enhanced DOR expression parallel to the maturation of cardiac parasympathetic, sympathetic, and sensory innervation of the heart suggests that the cardiac opioid system is an important regulator of neonatal and adult heart function through the autonomic nervous system.

show abstract

Section: D-opioid Receptors In the Developing Rat Heartsupporting

confidence: 95%

Developmental expression of δ‐opioid receptors during maturation of the parasympathetic, sympathetic, and sensory innervations of the neonatal heart: Early targets for opioid regulation of autonomic control

Mousa

Shaqura

Schäper

et al. 2011

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…The possibility that these differences were due to the different methods of field stimulation used for atria and ventricles is unlikely, as the response to clonidine, a prejunctional inhibitory agonist, was similar in the two tissues. Also, we have previously observed that dynorphin, an opioid agonist able to inhibit the adrenergic response to field stimulation, was active in ventricles at lower concentrations than in atria (Ledda et al, 1985;Mantelli et al, 1987). Thus, it seems reasonable to conclude that the inhibitory effect of prostaglandins observed in the present study was stronger in atrial than in ventricular preparations.…”

Section: Discussioncontrasting

confidence: 34%

“…1985). The main reasons for this modification were the different responsiveness of atria and ventricles: in fact we previously observed that the total number of field pulses able to evoke a sympathetic response in atria was remarkably lower than that required for ventricular tissue, probably as a consequence of the greater innervation of atria.…”

Section: Introductionmentioning

confidence: 99%

Different effects of prostaglandins on adrenergic neurotransmission in atrial and ventricular preparations

Mantelli

Amerini

Ledda

1990

British J Pharmacology

Self Cite

View full text Add to dashboard Cite

1 The effects of prostaglandin E2 (PGE2) and iloprost on the cardiac response to adrenergic nerve stimulation in guinea-pig atrial and ventricular preparations have been studied.2 In guinea-pig isolated atria both PGE2 (0.1-1O nM) and iloprost (0.1-3 gM) concentration-dependently reduced the cardiac response to adrenergic nerve stimulation. 3 The inhibition of cyclo-oxygenase by indomethacin and acetylsalicylic acid potentiated the response to nerve stimulation in the atrial preparations. 4 Arachidonic acid (i-IOMm) reduced the response to nerve stimulation in atria. This effect was prevented by indomethacin and acetylsalicylic acid. 5 In guinea-pig ventricles PGE2 and iloprost were found to be effective at higher concentrations than in atrial preparations: arachidonic acid, indomethacin or acetylsalicylic acid did not modify the cardiac response to adrenergic nerve stimulation. 6 These results suggest a different modulator role for endogenous prostaglandins in atrial and ventricular tissue.

show abstract

“…(1979), is proposed to be the endogenous ligand for the ~c-receptor (Chavkin et al, 1982). According to earlier morphological studies, dynorphin has mainly been found in different parts of the CNS, but also in peripheral tissues, such as the pancreas (Cetin, 1990), throughout the gastro-intestinal (GI) tract (Ambinder et al, 1979;Kamikawa etal., 1983;Costa et al, 1985;Wolter, 1985), the vas deferens (Oka et al, 1980) and in the circulatory system (Illes et al, 1983;Ledda et al, 1985). There are also reports of involvement of ~:-receptors in the central control of micturition (Sheldon et al, 1987).…”

Section: Introductionmentioning

confidence: 92%

Possible regulatory role of dynorphin A in the urinary bladder

Berggren¹,

Dahlström²,

Rubenson³

et al. 1992

J. Neural Transmission

View full text Add to dashboard Cite

show abstract

Sensitivity to dynorphin-(1–13) of the presynaptic inhibitory opiate receptors of the guinea-pig heart

Cited by 36 publications

References 9 publications

Developmental expression of δ‐opioid receptors during maturation of the parasympathetic, sympathetic, and sensory innervations of the neonatal heart: Early targets for opioid regulation of autonomic control

Developmental expression of δ‐opioid receptors during maturation of the parasympathetic, sympathetic, and sensory innervations of the neonatal heart: Early targets for opioid regulation of autonomic control

Different effects of prostaglandins on adrenergic neurotransmission in atrial and ventricular preparations

Possible regulatory role of dynorphin A in the urinary bladder

Contact Info

Product

Resources

About