Sensitivity Study of Cancer Antigens (CA-125) Detection Using Interdigitated Electrodes Under Microfluidic Flow Condition

Nunna, Bharath Babu; Mandal, Debdyuti; Lee, Joo Un; Zhuang, Shiqiang; Lee, Eon Soo

doi:10.1007/s12668-018-0589-1

Cited by 11 publications

(7 citation statements)

References 42 publications

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“…Next, AuNPs‐APTES acts as a linker during immobilization by a binding amine group (–NH2) with carboxylic acid‐modified on the virus ssDNA probe. The condensation process successfully helped in binding the AuNPs‐APTES and virus ssDNA 61 . The covalent bonds formed with the linker from the APTES solution are called amide bonds.…”

Section: Resultsmentioning

confidence: 99%

Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor‐based gold nanoparticles

Jaapar

Parmin

Halim

et al. 2021

Biotech and App Biochem

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The E6 region has higher protuberant probability annealing than consensus probe focusing on another region in the human papillomavirus (HPV) genome in terms of detection and screening method. Here, we designed the first multiple virus single‐stranded deoxyribonucleic acid (ssDNA) for multiple detections in an early phase of screening for cervical cancer in the E6 region and became a fundamental evolution of detection electrochemical HPV biosensor. Gene profiling of the virus ssDNA sequences has been carried by high‐end bioinformatics tools such as GenBank, Basic Local Alignment Searching Tools (BLAST), and Clustal OMEGA in a row. The output from bioinformatics tools resulted in 100% of similarities between our virus ssDNA probe and HPV complete genome in the databases. The cross‐validation between HPV genome and our designed virus ssDNA provided high specificity and selectivity during screening methods compared with Pap smear. The DNA probe for HPV 18, 5′ COOH‐GAT CCA GAA GGT ACA GAC GGG GAG GGC ACG 3′, while 5′COOH‐GGG CGC TGT GCA GTG TGT TGG AGA CCC CGA3′ as DNA probe for HPV 58 designed with 66.77% guanine (G) and cytosine (C) content for both. Our virus ssDNA probe for the HPV biosensor promises high sensitivity, specificity, selectivity, repeatability, low fluid consumption, and will be useful in mini‐size diagnostic devices for cervical cancer detection.

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Section: Resultsmentioning

confidence: 99%

Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor‐based gold nanoparticles

Jaapar

Parmin

Halim

et al. 2021

Biotech and App Biochem

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“…This type of technology is based on the microfluidic flow of biofluid to detect a change in capacitance. The signals are amplified and obtained from an electrode platform based on antigen and antibody interaction [192]. Other types of biosensors use carbon nanotubes to enhance the signal and sensitivity levels of biomarker disease detection [193,194].…”

Section: Challenges Currently Facing Tests On the Marketmentioning

confidence: 99%

COVID-19 Biomarkers and Advanced Sensing Technologies for Point-of-Care (POC) Diagnosis

et al. 2021

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COVID-19, also known as SARS-CoV-2 is a novel, respiratory virus currently plaguing humanity. Genetically, at its core, it is a single-strand positive-sense RNA virus. It is a beta-type Coronavirus and is distinct in its structure and binding mechanism compared to other types of coronaviruses. Testing for the virus remains a challenge due to the small market available for at-home detection. Currently, there are three main types of tests for biomarker detection: viral, antigen and antibody. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) remains the gold standard for viral testing. However, the lack of quantitative detection and turnaround time for results are drawbacks. This manuscript focuses on recent advances in COVID-19 detection that have lower limits of detection and faster response times than RT-PCR testing. The advancements in sensing platforms have amplified the detection levels and provided real-time results for SARS-CoV-2 spike protein detection with limits as low as 1 fg/mL in the Graphene Field Effect Transistor (FET) sensor. Additionally, using multiple biomarkers, detection levels can achieve a specificity and sensitivity level comparable to that of PCR testing. Proper biomarker selection coupled with nano sensing detection platforms are key in the widespread use of Point of Care (POC) diagnosis in COVID-19 detection.

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“…Other studies employed the immunosensing strategy based on the detection of carcinoma antigen (CA) 125, as the most widely used glycoprotein for the early diagnosis of ovarian cancer [ 87 , 88 ]. On the one hand, Nami et al developed a paper-based immunosensor by depositing silver nanoparticles onto reduced graphene oxide sheets and subsequently attaching cysteamine-functionalized gold nanoparticles that will further bond with the CA 125 antibody [ 87 ].…”

Section: Diagnostic Applicationsmentioning

confidence: 99%

“…On the one hand, Nami et al developed a paper-based immunosensor by depositing silver nanoparticles onto reduced graphene oxide sheets and subsequently attaching cysteamine-functionalized gold nanoparticles that will further bond with the CA 125 antibody [ 87 ]. On the other hand, Nunna et al developed a self-assembled monolayer by treating gold electrodes with thiourea for the subsequent attachment of the CA 125 antibody through the amine groups [ 88 ]. Similarly, Zheng et al immobilized silver nanoparticles onto the surface of the microchannel for creating a SERS-active substrate that would allow for the attachment of antibodies to capture the target biomarkers CA 125, CA 153 simultaneously, and a carcinoembryonic antigen for breast cancer diagnosis [ 89 ].…”

Section: Diagnostic Applicationsmentioning

confidence: 99%

Biosensors-on-Chip: An Up-to-Date Review

et al. 2020

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Generally, biosensors are designed to translate physical, chemical, or biological events into measurable signals, thus offering qualitative and/or quantitative information regarding the target analytes. While the biosensor field has received considerable scientific interest, integrating this technology with microfluidics could further bring significant improvements in terms of sensitivity and specificity, resolution, automation, throughput, reproducibility, reliability, and accuracy. In this manner, biosensors-on-chip (BoC) could represent the bridging gap between diagnostics in central laboratories and diagnostics at the patient bedside, bringing substantial advancements in point-of-care (PoC) diagnostic applications. In this context, the aim of this manuscript is to provide an up-to-date overview of BoC system development and their most recent application towards the diagnosis of cancer, infectious diseases, and neurodegenerative disorders.

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Sensitivity Study of Cancer Antigens (CA-125) Detection Using Interdigitated Electrodes Under Microfluidic Flow Condition

Cited by 11 publications

References 42 publications

Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor‐based gold nanoparticles

Designing DNA probe from HPV 18 and 58 in the E6 region for sensing element in the development of genosensor‐based gold nanoparticles

COVID-19 Biomarkers and Advanced Sensing Technologies for Point-of-Care (POC) Diagnosis

Biosensors-on-Chip: An Up-to-Date Review

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