Sensitivity of Various Salmonella Strains to Felix 0–1 Phage

Kallings, Lars Olof

doi:10.1111/j.1699-0463.1967.tb01312.x

Cited by 35 publications

(10 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After first descriptions in the 1960's, bacteriophages are currently again in the focus of scientists. The Felix O1 bacteriophage in particular appears to be relatively salmonella-specific and sensitive: 99.5 % of salmonella cultures were lysed by this phage, with only less than 0.3 % of other Enterobacteriaceae being affected [14]. In another study, 98.2 % of salmonella strains isolated from human patients were lysed, while only 1.4 % of other Enterobacteriaceae were also lysed [35].…”

Section: Introductionmentioning

confidence: 92%

The use of a salmonella bacteriophage in bearded dragons: application, passage time and reisolation

Renfert¹,

Rabsch

Fruth

et al. 2019

Tierarztl Prax Ausg K

View full text Add to dashboard Cite

Objective This study determined the passage time and phage propagation time of a salmonella specific phage, Felix O1, in bearded dragons, based on reisolation from cloacal swabs and faecal samples following oral administration, as a possible tool for reducing the zoonotic risk of salmonella from pet reptiles. An application scheme for this phage in bearded dragons was developed. Material and methods Ten healthy bearded dragons (Pogona vitticeps) were used in the study. The pH tolerance of the phage was tested and drugs were used to evaluate their influence on the gastric pH of the reptiles. After pH adjustment, the phage was administered orally for 12 consecutive days. Over 60 days, swabs were taken from the cloaca and examined for the presence of phages using culture and PCR. Furthermore, faecal samples were collected for phage quantification. Results Felix O1 displayed no activity at pH below 2.8. A calcium- and magnesium carbonate buffer induced an appropriate gastric pH increase for 30 minutes. Phages were reisolated for up to 24 days (mean shedding: 19 days) after last administration. Titres between 105 and 107 plaque forming units/g faeces were detected. The animals did not show any clinical signs related to phage application. Conclusion and clinical relevance The study provides first results on oral administration, passage time, and reisolation of a phage in reptiles. It could be shown that the phage was able to replicate in the intestine, and was shed for a prolonged period and therefore could potentially contribute to a reduction of salmonella shedding.

show abstract

Section: Introductionmentioning

confidence: 92%

The use of a salmonella bacteriophage in bearded dragons: application, passage time and reisolation

Renfert¹,

Rabsch

Fruth

et al. 2019

Tierarztl Prax Ausg K

View full text Add to dashboard Cite

show abstract

“…of a phage is the highest dilution which produces confl uent or semi-confl uent lysis on its homologous type strain, the strain on which the phage had been propagated. If the phages were to be used undiluted many of the reactions might be nonspecifi c [ 202 ]. Some of the phages producing small or minute plaques may only have to be diluted to 10 −3 , whereas others that produce large plaques can be diluted to 10 −5 or 10 −6 to obtain the R.T.D.…”

Section: Phage Typing Proceduresmentioning

confidence: 99%

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Switt

Sulakvelidze

Wiedmann

et al. 2014

Methods in Molecular Biology

View full text Add to dashboard Cite

Since this book was originally published in 2007 there has been a significant increase in the number of Salmonella bacteriophages, particularly lytic virus, and Salmonella strains which have been fully sequenced. In addition, new insights into phage taxonomy have resulted in new phage genera, some of which have been recognized by the International Committee of Taxonomy of Viruses (ICTV). The properties of each of these genera are discussed, along with the role of phage as agents of genetic exchange, as therapeutic agents, and their involvement in phage typing.

show abstract

“…The expected result would have been a synergistic effect of each different phages against the Salmonella infection (15,90,95,145) The results observed in this trial indicated a trend in reducing the level of Salmonella by administration route of the phage cocktail (Table 4 and In light of these results, a second approach was carried out to search for a single phage that is capable of lysing a broad range of Salmonella. An in vitro assay showed that F0-1 phage effectively lysed the serovars of Salmonella most frequently found on swine farms (Table 6) and multi-drug resistant strains of Salmonella serovar Typhimurium (Table 7), consistent with previous studies (61,(85)(86)(87). It was found that F0-1 phage can adsorb (Table 8), which agreed with previous studies (51, 74,75).…”

Section: Chapter 5 Discussionsupporting

confidence: 90%

Reduction of Salmonella enterica serovar Typhimurium in swine by bacteriophage treatment

Lee

View full text Add to dashboard Cite

show abstract

Sensitivity of Various Salmonella Strains to Felix 0–1 Phage

Cited by 35 publications

References 4 publications

The use of a salmonella bacteriophage in bearded dragons: application, passage time and reisolation

The use of a salmonella bacteriophage in bearded dragons: application, passage time and reisolation

Salmonella Phages and Prophages: Genomics, Taxonomy, and Applied Aspects

Reduction of Salmonella enterica serovar Typhimurium in swine by bacteriophage treatment

Contact Info

Product

Resources

About