Sensitivity of the amide I band to matrix manipulation in bone: a Raman micro-spectroscopy and spatially offset Raman spectroscopy study

Ahmed, Rafay; Ünal, Mustafa; Gautam, Rekha; Uppuganti, Sasidhar; Derasari, Shrey; Mahadevan‐Jansen, Anita; Nyman, Jeffry S.

doi:10.1039/d3an00527e

Cited by 4 publications

(1 citation statement)

References 52 publications

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“…Raman spectroscopic data was acquired i) before and after tensile tests, ii) from control and glucose incubated beams. RS was conducted as previously described 19 . Briefly, Raman system (Renishaw InVia Raman microscope) equipped with 785nm excitation laser and a holographic grating of 1200 lines/mm providing spectral resolution of ~1 cm −1 was used for acquisition.…”

Section: Raman Spectroscopy (Rs)mentioning

confidence: 99%

Raman spectroscopic assessment of glycation effect and mechanical damage induced in human cortical bone

Ahmed,

Voziyan,

Uppuganti

et al. 2024

Biomedical Vibrational Spectroscopy 2024: Advances in Research and Industry

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Bone has complex hierarchical structure composed of mineral, collagen, lipids, water and non-collagenous proteins. Being able to probe at the ultrastructural level of bone hierarchy is important to understand age-and disease-related changes in the fracture resistance. Raman spectroscopy (RS) probes molecular vibration and is sensitive to advanced glycation end-products, damage to the bone matrix, thus fracture toughness properties. We studied effect of glycation and mechanical damage on characteristics of Amide I Band. N=20 cadaveric femurs were obtained from Vanderbilt Cadaver Program. We extracted medial and lateral quadrants of cortical bone (~50 mm) along the longitudinal axis of mid-shaft using saw. Dog-bone specimens were created for tensile tests while 2-rectangular beams for glycation incubation. a) For tensile tests, specimens were pulled-to-failure at 5mm/min using 1000 N load cell. b) For glycation, beam-1 (control) was incubated in 50 mM Na3PO4, 0.02% NaN3, protease inhibitor cocktail, and beam-2 (Glucose) with same buffer plus 0.5 M glucose for 5-weeks at 45 0 C, pH 7.6. RS acquired before and after both tests indicates that Amide I sub-peak ratio 1670/1640 significantly increased post tensile tests compared to baseline. 1670 and 1640 cm -1 peaks are associated with random coils and alpha helix respectively. Thus, more disorganized structures are formed because of tensile damages. Glycated beams show lower 1670/1640 ratio compared to baseline and control. This in part due to increase in packing density of collagen molecules upon glycation. Overall, our results indicate that RS can assess cortical damage and sugar-mediated changes in the amide I band.

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Section: Raman Spectroscopy (Rs)mentioning

confidence: 99%