Abstract. The present study explores the effect of weed management practices on the properties of soil, including microbial biomass carbon (MBC), soil organic carbon (SOC), basal respiration (BR), induced respiration (IR), and microbial quotient (MQ) in potato cultivation at two agricultural research stations of Alarogh and Samian in Ardabil Province, Iran. We carried out a randomized complete block design with three replications. The treatments were as follows: 1) Trifluralin herbicide (TR), 2) Metribuzin herbicide (ME), 3) cultivator practice (CU), 4)wheat straw mulch (WH), 5) canola straw mulch (CA), 6) black plastic mulch (BPL), 7) transparent plastic mulch (TPL), 8) weed infested (WI), and 9) weed free (WF). Soil sampling was conducted at three stages. The first stage included control index sampling (CIS). The treatments employed in this research caused significant differences in the rates of MBC, SOC, BR, IR, and MQ. The highest rate of MBC was observed in WH and CA at the second and the third soil sampling stages, respectively, compared with other experimental treatments. WH and CA increased the rate of MBC in the second and the third sampling stages in comparison with CIS. The highest SOC was observed in WH and CA at the second and the third soil sampling stages. The highest BR at the second sampling stage was related to CA, WH, and BPL; however, the lowest BR at the second soil sampling stage was related to TR. Furthermore, the highest BR rate at the third sampling stage belonged to WH, CA, and ME. Nevertheless, IR had the highest rate in WH and CA and the lowest rate in the CU at the second sampling stage. Hence, we found that applying plant straw mulch treatments to cover the soil surface for weed management, compared to other methods employed in the experiment, had more positive effects on the biological characteristics measured.