Sensitivity of soil fungal and bacterial community compositions to nitrogen and phosphorus additions in a temperate meadow

Yan, Yan; Sun, Xiaochuan; Sun, Fengwei; Zhao, Yinan; Sun, Wei; Guo, Jixun; Zhang, Tao

doi:10.1007/s11104-021-05237-9

Cited by 26 publications

(20 citation statements)

References 67 publications

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“…However, a recent meta-analysis showed that soil fungal diversity was more sensitive to N addition than bacterial diversity (Yang et al, 2020). This is supported by Yan et al (2021), which reported that the N addition showed non-significant effects on soil bacterial richness and community composition but significantly increased soil fungal richness in a temperate meadow. These varying results also suggest that the responses of different soil microbial groups to nutrient enrichment might be ecosystem specific.…”

Section: Introductionmentioning

confidence: 89%

“…Considerable works have been conducted to explore how N additions influence soil microbial communities (Lu et al, 2021;Yan et al, 2021). These studies generally suggested that N enrichment could affect soil microbial diversity and composition by altering soil pH, plant diversity, and soil N availability (Weand et al, 2010;Yang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Notably, many ecosystems are experiencing simultaneously enrichment of N and P, and quantifying the interactive effects of N and P have been considered to be crucial for predicting the responses of terrestrial ecosystems to nutrient enrichments (Jiang et al, 2019;Yan et al, 2021). Yue et al (2017) have demonstrated that N+P additions largely caused additive effects on soil C storage at the global scale; and another meta-analysis showed that co-additions of N and P have uniformly synergistic effects on aboveground plant biomass across different terrestrial ecosystems (Jiang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Although there have been some studies investigating the effects of N and P enrichment in various ecosystems like tropical forests (Li et al, 2019), croplands (Zheng et al, 2020), and grasslands (Yan et al, 2021), scarce information is available on how N and P additions interactively affect soil bacterial and fungal community in riparian ecosystems. 10.3389/fmicb.2022.999385 Riparian ecosystems have been considered as the effective sink for N and P pollutants from agricultural runoff (Lyu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Soil bacteria are more sensitive than fungi in response to nitrogen and phosphorus enrichment

et al. 2022

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Anthropogenic activities have dramatically increased nitrogen (N) and phosphorous (P) enrichments in terrestrial ecosystems. However, it is still unclear on how bacterial and fungal communities would respond to the simultaneously increased N and P enrichment. In this study, we used a field experiment to simulate N and P input, and examined the effects of N and P additions on the abundance, alpha-diversity, and community composition of soil bacteria and fungi in a riparian forest. Six nutrient-addition treatments, including low N (30 kg N ha–1 year–1), high N (150 kg N ha –1 year–1), low P (30 kg P2O5 ha–1 year–1), high P (150 kg P2O5 ha –1 year–1), low N+P, high N+P, and a control (CK) treatment were set up. We found that the N and P additions significantly affected bacterial abundance, community composition, but not the alpha diversity. Specifically, 16S, nirK, and nirS gene copy numbers were significantly reduced after N and P additions, which were correlated with decreases in soil pH and NO-3-N, respectively; Co-additions of N and P showed significantly antagonistic interactions on bacterial gene copies; Nutrient additions significantly increased the relative abundance of Proteobacteria while reduced the relative abundance of Chloroflexi. Mantel’s test showed that the alteration in bacterial composition was associated with the changes in soil pH and NO-3-N. The nutrient additions did not show significant effects on fungal gene copy numbers, alpha diversity, and community composition, which could be due to non-significant alterations in soil C/N and total P concentration. In conclusion, our results suggest that soil bacteria are more sensitive than fungi in response to N and P enrichment; the alterations in soil pH and NO-3-N explain the effects of N and P enrichment on bacterial communities, respectively; and the co-addition of N and P reduces the negative effects of these two nutrients addition in alone. These findings improve our understanding of microbial response to N and P addition, especially in the context of simultaneous enrichment of anthropogenic nutrient inputs.

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Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Soil bacteria are more sensitive than fungi in response to nitrogen and phosphorus enrichment

et al. 2022

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“…Nitrogen (N) and phosphorus (P) availability often constrain the growth and metabolism of plant and soil microorganisms in terrestrial ecosystems (Dijkstra et al, 2012 ; Yuan and Chen, 2015 ; Jiang et al, 2021 ). Supplies and inputs of N and P have been greatly affected by pre-industrial revolution and human activities (e.g., fossil combustion and excessive fertilizer applications) (Ma et al, 2016 ; Yan et al, 2022 ). Presently, global inputs of N and P have increased by 84.0 and 43.3%, respectively, relative to the input before the 20th century (Peñuelas et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Soil properties rather than plant diversity mediate the response of soil bacterial community to N and P additions in an alpine meadow

et al. 2022

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The alpine meadow on the Qinghai-Tibetan Plateau, which is susceptible to global climate change and human activities, is subject to nutrient addition such as nitrogen (N) and phosphorus (P) to enhance soil available nutrients and ecosystem productivity. Soil bacterial community partly drivers the effects of nutrient additions on ecosystem processes, whereas the factors influencing N and P additions on bacterial community in alpine meadows are not well documented. We conducted a N and P addition experiment in an alpine meadow ecosystem on the Qinghai-Tibetan Plateau with four treatments: untreated control (CK), N addition (N), P addition (P), and NP addition (NP). We employed a high-throughput Illumina Miseq sequencing technology to investigate the response of soil bacterial community to short-term N and P additions. N and P additions decreased soil bacterial richness (OTU numbers and Chao 1 index), and P addition decreased soil bacterial diversity (Shannon and Simpson indices). N addition directly induced the change of soil NH4+−N, and decreased plant diversity. The N and P additions reduced soil bacterial community diversity, whose response was independent with plant diversity. Additionally, nutrient additions altered soil bacterial community composition, which were highly correlated with soil properties (i.e. pH, NH4+−N, and TP) as shown by RDA. Consistently, structural equation modeling results revealed that N addition indirectly acted on soil bacterial community through altering soil available nutrients and pH, while P addition indirectly affected bacterial community by increasing soil P availability. These findings imply that more attention should be paid to soil properties in regulating belowground biodiversity process in alpine meadows under future environmental change scenario.

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Distinct responses of soil bacterial communities to N and P fertilization in a Tibetan alpine meadow of China

et al. 2023

Land Degrad Dev

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Increased nitrogen (N) and phosphorus (P) availability, due to fertilization and deposition, is expected to influence soil microbial community structure and function. However, little is known about the sensitivity and dependency of soil microbial structure on the levels of N and P, and on the integration of N and P inputs in alpine meadow ecosystems. Here, we implemented a 3‐year manipulative fertilization study with four levels (0, 10, 20, and 30 g m−2) of N fertilization, P fertilization, and NP fertilization (N and P fertilization at a ratio of 1:1) in a Tibetan alpine meadow. We examined how greater N and P availability affected bacterial community diversity, community structure in the top‐ (0–10 cm) and sub‐top (10–20 cm) soil, network topological properties, and the potential mechanisms behind them. Although the α and β diversities of bacteria within top‐ and sub‐topsoil were not significantly different from those in the control across N, P, and NP fertilization levels, the integration of NP fertilization significantly changed the relative abundances of more bacterial phyla than the N and P fertilization did separately. Furthermore, the N10 and N20 fertilization treatments strengthened bacterial interactions, whereas P and NP fertilization did not affect the complexity and connectivity of the soil bacterial co‐occurrence network. In addition, the mechanism of structuring the bacterial community for separate N and P fertilization differed from that for NP fertilization. The separate application of N and P fertilization affected the bacterial community composition mainly through changes in the total and available P levels in the soil, respectively, whereas NP fertilization shifted the bacterial community composition through decreasing plant species richness. These findings provide a quantification of the response of soil bacterial diversity and composition to different levels of N, P, and NP fertilization. It also suggests that in the Tibetan alpine meadow, soil bacterial communities may alter their fitness according to the relative abundances of oligotrophic/copiotrophic bacterial taxa, rather than through changing their diversity.

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Sensitivity of soil fungal and bacterial community compositions to nitrogen and phosphorus additions in a temperate meadow

Cited by 26 publications

References 67 publications

Soil bacteria are more sensitive than fungi in response to nitrogen and phosphorus enrichment

Soil bacteria are more sensitive than fungi in response to nitrogen and phosphorus enrichment

Soil properties rather than plant diversity mediate the response of soil bacterial community to N and P additions in an alpine meadow

Distinct responses of soil bacterial communities to N and P fertilization in a Tibetan alpine meadow of China

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