Sensitivity of Ocular Anterior Chamber Tissues to Oxidative Damage and Its Relevance to the Pathogenesis of Glaucoma

Izzotti, Alberto; Saccà, Sergio Claudio; Longobardi, M.; Cartiglia, Cristina

doi:10.1167/iovs.09-3871

Cited by 129 publications

(104 citation statements)

References 56 publications

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“…This issue is relevant because we recently demonstrated that TM is the most sensitive tissue to in vitro oxidative stress in the anterior chamber. 13 The quantification of mtDNA 4977 was carried out by quantitative polymerase chain reaction (QPCR), which is highly sensitive and reliable as confirmed by the increasing number of articles using this method. 14,15 Insofar, evidence demonstrating the purported role of mitochondrial damage in POAG pathogenesis is overwhelming, but most results were not obtained using POAG target tissue, ie, the TM.…”

Section: He Trabecular Meshworkmentioning

confidence: 98%

Mitochondrial Damage in the Trabecular Meshwork of Patients With Glaucoma

et al. 2010

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Section: He Trabecular Meshworkmentioning

confidence: 98%

Mitochondrial Damage in the Trabecular Meshwork of Patients With Glaucoma

et al. 2010

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“…Recent reports that vitrectomy and cataract surgery can increase oxygen delivery to the outflow pathway and directly increases oxidative stress (Chang, 2006;Luk et al, 2009;Siegfried et al, 2010). Oxidative stress may also present an important pathogenic step in primary open-angle glaucoma by inducing human trabecular meshwork degeneration, favouring an intraocular pressure (IOP) increase, thus priming the glaucoma pathogenic cascade (Izzotti et al, 2009;Sacca et al, 2005). The iris microvasculature being the predominant source of oxygen in the region most likely plays a critical role in maintaining oxygen homeostasis.…”

Section: Introductionmentioning

confidence: 99%

Quantitative study of the microvasculature and its endothelial cells in the porcine iris

Yang

Cringle

et al. 2015

Experimental Eye Research

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“…Recent reports that intraocular surgery can disturb the intraocular oxygen gradients, increase oxygen delivery to the outflow pathway and directly induce oxidative stress (Chang, 2006;Luk et al, 2009;Siegfried et al, 2010). Oxidative stress may also present an important pathogenic step in primary open-angle glaucoma by inducing trabecular meshwork degeneration, favouring an intraocular pressure (IOP) increase, thus priming the glaucoma pathogenic cascade (Izzotti et al, 2009;Sacca et al, 2005). Hence, any iris vascular abnormality would probably affect the Fig.…”

Section: Discussionmentioning

confidence: 99%

Intracellular cytoskeleton and junction proteins of endothelial cells in the porcine iris microvasculature

Yang

Cringle

et al. 2015

Experimental Eye Research

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Recently we reported studies of the iris microvasculature and its endothelial cells using intra-luminal micro-perfusion, fixation, and silver staining, suggesting that the iris vascular endothelium may be crucial for maintaining homeostasis in the ocular anterior segment. Here we present information regarding the intracellular structure and cell junctions of the iris endothelium. Thirty-seven porcine eyes were used for this study. The temporal long posterior ciliary artery was cannulated to assess the iris microvascular network and its endothelium using intra-luminal micro-perfusion, fixation, and staining with phalloidin for intracellular cytoskeleton f-actin, and with antibodies against claudin-5 and VE-cadherin for junction proteins. Nuclei were counterstained with Hoechst. The iris was flat-mounted for confocal imaging. The iris microvasculature was studied for its distribution, branch orders and endothelial morphometrics with endothelial cell length measured for each vessel order. Our results showed that morphometrics of the iris microvasculature was comparable with our previous silver staining. Abundant stress fibres and peripheral border staining were seen within the endothelial cells in larger arteries. An obvious decrease in cytoplasmic stress fibres was evident further downstream in the smaller arterioles, and they tended to be absent from capillaries and veins. Endothelial intercellular junctions throughout the iris vasculature were VE-cadherin and claudin-5 immuno-positive, indicating the presence of both adherent junctions and tight junctions between vascular endothelial cells throughout the iris microvasculature. Unevenness of claudin-5 staining was noted along the endothelial cell borders in almost every order of vessels, especially in veins and small arterioles. Our results suggest that significant heterogeneity of intracellular structure and junction proteins is present in different orders of the iris vasculature in addition to vascular diameter and shape of the endothelia. Detailed information of the topography and intracellular structure and junction proteins of the endothelium of the iris microvasculature combined with unique structural features of the iris may help us to further understand the physiological and pathogenic roles of the iris vasculature in relevant ocular diseases.

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Sensitivity of Ocular Anterior Chamber Tissues to Oxidative Damage and Its Relevance to the Pathogenesis of Glaucoma

Cited by 129 publications

References 56 publications

Mitochondrial Damage in the Trabecular Meshwork of Patients With Glaucoma

Mitochondrial Damage in the Trabecular Meshwork of Patients With Glaucoma

Quantitative study of the microvasculature and its endothelial cells in the porcine iris

Intracellular cytoskeleton and junction proteins of endothelial cells in the porcine iris microvasculature

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