Sensitivity of neurodegenerative mutants of Drosophila melanogaster from Swiss cheese group to the oxidative stress conditions

Mohylyak, Iryna; Matiytsiv, N. P.; Hrunyk, N. I.; Chernyk, Y. I.

doi:10.7124/bc.000117

Cited by 5 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resistance against prooxidant Н 2 О 2 . Experiment was performed as described earlier (Mohylyak et. al.,2011).…”

Section: Methodsmentioning

confidence: 99%

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melanogaster

Matiytsiv,

Raspopina,

Dronska

et al. 2023

Biol. Stud.

View full text Add to dashboard Cite

Background. The incidence of human neurodegenerative disorders increases continuously as the human population ages. To date, these diseases remain incurable and require complex experimental approaches using tractable models to study the degeneration mechanisms and potential drug intervention regimens. In the current work, we assessed the impact of the neuroprotective drug Сerebrolysin on these neurodegenerative processes in Drosophila Sod1 and swiss cheese (sws) mutants. Materials and Methods. The experiments were conducted using a D. melanogaster Sod1- and sws-dependent neurodegeneration model. Сerebrolysin (3 μL/mL) was added for larvae feeding. In order to evaluate Сerebrolysin influence, several tests were performed: locomotor activity assay, lifespan, size of brain tissue degeneration zones and sensitivity to prooxidant exposion. Results. Dietary supplementation with Сerebrolysin extended the lifespan of all flies under normal circumstances. The drug treatment also reduced the sensitivity of mutant flies to pro-oxidant effects. Moreover, treatment with Сerebrolysin partially diminished the size of degeneration zones in the brain tissue of sws1 mutant flies, without any notable effects on locomotor ability. Conclusions. The data obtained confirm the moderate neuroprotective and/or antioxidant action of Сerebrolysinagainst neurodegenerative processes under different genetic backgrounds.

show abstract

“…Resistance against prooxidant Н 2 О 2 . Experiment was performed as described earlier (Mohylyak et. al.,2011).…”

Section: Methodsmentioning

confidence: 99%

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melanogaster

Matiytsiv,

Raspopina,

Dronska

et al. 2023

Biol. Stud.

View full text Add to dashboard Cite

show abstract

A Private History of Neurogenetics: The swiss cheese Gene and Its Orthologs

2021

View full text Add to dashboard Cite

Morphological Changes in the Hippocampus of Rats in Accelerated Aging

2014

View full text Add to dashboard Cite

The aim of this work was the analysis of structural changes with age in the hippocampus of senescenceaccelerated OXYS rats when signs of accelerated brain aging are missing (age 14 days), developments (age 5 months), and active progresses (age 15 months). The study was performed on 15 OXYS rats and 15 Wistar rats (as a control). After dislocation, brains were dissected, fixed with 10% formalin, embedded in paraffin, and serially cut in coronal sections (5μm thickness). These sections were stained with Cresyl violet and examined with a photomicroscope (Carl Zeiss Axiostar plus, Germany). The total number of hippocampal pyramidal cells in the CA1, CA3 and the dentate gyrus regions were estimated in 14-dayold, 5and 15-month-old OXYS and Wistar rats (n = 5) on the 5 slices of each brain sections. The number of neurons with chromatolysis, hyperchromatic with darkly stained cytoplasm and shrunken neurons were calculated as degenerative neurons. The pictures obtained with the program Carl Zeiss Axio Vision 8.0 with increasing 10  100, determined the average area bodies and nuclei of neurons (mkm2). The significant structural changes of neurons in the CA1, CA3 and dentate gyrus regions of the hippocampus in OXYS rats at 5 month of age are revealed by light microscopy. This results indicates the early develop neurodegeneration in OXYS rats. The most pronounced morphological changes occur in the CA1 region of the hippocampus of OXYS rats and irreversible. The degenerative changes of neurons in the hippocampus increases by the age of 15 months. Morphometric analysis of the average area of bodies and the nuclei of hippocampal neurons in CA1, CA3 and the dentate gyrus regions of OXYS and Wistar rats at 14 days of age showed no significant interline differences. At 5 months of age in the CA1 region of the hippocampus of OXYS rats was determined a significantly lower average body size and nuclei of pyramidal neurons compared with Wistar rats. With age, these changes have progressed and 15-month-old OXYS rats have a significant decrease in the area bodies and nuclei in all studied regions of the hippocampus compared with Wistar rats. These results confirm the promising use OXYS rats as a model of accelerated brain.

show abstract

Sensitivity of neurodegenerative mutants of Drosophila melanogaster from Swiss cheese group to the oxidative stress conditions

Cited by 5 publications

References 19 publications

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melanogaster

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melanogaster

A Private History of Neurogenetics: The swiss cheese Gene and Its Orthologs

Morphological Changes in the Hippocampus of Rats in Accelerated Aging

Contact Info

Product

Resources

About

Sensitivity of neurodegenerative mutants of Drosophila melanogaster from Swiss cheese group to the oxidative stress conditions

Cited by 5 publications

References 19 publications

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melano­gaster

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melano­gaster

A Private History of Neurogenetics: The swiss cheese Gene and Its Orthologs

Morphological Changes in the Hippocampus of Rats in Accelerated Aging

Contact Info

Product

Resources

About

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melanogaster

Сerebrolysin® influences in Sod- and sws-dependent neurodegenerative models of Drosophila melanogaster