Sensitivity of Neisseria Gonorrhoeae to Partially Purified R-Type Pyocines and a Possible Approach to Epidemiological Typing

Sensitivity of Thermophilic Campylobacters to R-type Pyocines of Pseudomonas Aeruginosa

“…The receptor site for R-type pyocines is in the lipopolysaccharide (LPS) of the cell envelope of P. aeruginosa (Ikeda and Egami, 1973;Govan, 1974;Koval and Meadow, 1977) and a similar location has been suggested in Neisseria (Sidberry and Sadoff, 1977;Sadoff, Zollinger and Sidberry, 1978;Blackwell et al, 1979). Because campylobacter cell walls closely resemble those of other gram-negative organisms, similar receptors for R-type pyocines may exist in the LPS of campylobacters.…”

“…aeruginosa (Gillies and Govan, 1966), strains of N. gonorrhoeae (Blackwell et al, 1979) and non-serogroupable N. meningitidis (Blackwell and Law, 1981). As far as we are aware, the sensitivity of thermophilic campylobacters to R-type pyocines has not been reported previously.…”

“…The R-type pyocines were induced and partially purified as described previously (Blackwell et al, 1979), after the method of Govan (1974). Sensitivity to R-type pyocines was determined by placing drops (15 pl) of a 1 in 2 dilution of each pyocine preparations on to plates of (1) tryptone soya agar supplemented with 5% (v/v) of defibrinated horse-blood, (2) nutrient agar (Columbia Agar Base, Oxoid) containing agar 1% (w/v), and (3) similar nutrient agar containing agar 2% (w/v).…”

“…In this study we examined two possible methods for typing thermophilic campylobacters: examining strains for bacteriocine activity, and testing their sensitivity to partially purified preparations of rod-type (R-type) pyocines of Pseudomonas aeruginosa, which are of use for typing Neisseria gonorrhoeae (Blackwell, Young and Anderson, 1979), and non-serogroupable strains of N. meningitidis (Blackwell and Law, 1981).…”

Absence of bactericidal antibodies against group-I lipopolysaccharide determinants of Neisseria gonorrhoeae during human infection

“…Briefly, gonococcal strains sensitive to pyocins of Kageyama's group R5, lipopolysaccharide (LPS) Group I, are resistant to normal human serum and they bind in lower numbers to polymorphonuclear leukocytes than strains of Group I1 . This second group is sensitive to pyocins of Kageyama's group R1 in addition to R5 and strains of this group are killed by the majority of normal human sera tested (Kageyama, 1975;Blackwell, Young and Anderson, 1979;Winstanley et al, 1983). Strains of Group I1 are thought to have extra LPS components, absent or cryptic in strains of Group I, to which there are bactericidal antibodies in the majority of normal human sera (Schneider et al, 1982;.…”