Sensitivity of Koilocytosis, Immunocytochemistry, and Electron Microscopy as Compared to DNA Hybridization in Detecting Human Papillomavirus in Cervical and Vaginal Condyloma and Intraepithelial Neoplasia

Sato, Shinji; Okagaki, Takashi; Clark, Barbara A.; Twiggs, Leo B.; Fukushima, Michio; Ostrow, Ronald S.; Faras, Anthony J.

doi:10.1097/00004347-198612000-00002

Cited by 36 publications

(13 citation statements)

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“…Thus, the percentage of antigen-positive specimens decreases as the severity of the disease increases from mild dysplasia to carcinoma in situ to invasive carcinoma (45,84,125,164,183,187,194,199). In fact, the percentage of antigen-positive cells in adjacent condylomatous tissue decreases as the severity of the lesion increases (57,84).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Human papillomaviruses: are we ready to type?

Roman

Fife

1989

Clin Microbiol Rev

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The issue of determining which human papillomavirus (HPV) is present in a clinical specimen (typing specimens for HPVs) is receiving attention because HPVs cause condyloma acuminata and are associated with the continuum of disease which ranges from dysplasia to invasive genital cancer. Morphological inspection of precancerous lesions is not sufficient to determine which lesions will progress and which will not. A number of research tools based primarily on deoxyribonucleic acid hybridization have been developed. These permit identification and typing of HPV in genital tract scrapings or biopsies. Some HPV types (e.g., HPV-16 and HPV-18) have been identified in high-grade dysplasias and carcinomas more commonly than other types (e.g., HPV-6) and have been designated "high risk" types for cervical cancer. Thus, the question arises whether HPV typing would improve patient management by providing increased sensitivity for detection of patients at risk or by providing a prognostic indicator. In this review, the available typing methods are reviewed from the standpoint of their sensitivity, specificity, and ease of application to large-scale screening programs. Data implicating HPVs in the genesis of genital tract cancers are reviewed, as is the association of specific HPV types with specific outcomes. We conclude that there is currently no simple, inexpensive assay for HPV types, although such assays may be developed in the future. Analysis of the typing data indicates that, while HPV types can be designated high risk and low risk, these designations are not absolute and thus the low-risk group should not be ignored. In addition, interpretation of the data is complicated by finding high-risk types in individuals with no indication of disease. Insufficient data exist to indicate whether knowledge of the presence of a given HPV type is a better prognostic indicator than cytological or histological results. Thus, more research is needed before it can be determined whether typing information will augment the method currently in use for deciding treatment regimen and whether it warrants widespread use.

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Section: Immunocytochemistrymentioning

confidence: 99%

Human papillomaviruses: are we ready to type?

Roman

Fife

1989

Clin Microbiol Rev

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“…47 HPV-18 is more consistently associated with adenocarcinomas and small-cell neuroendocrine cancers of the cervix and relatively less frequently with invasive squamous cancer, although in absolute terms HPV 18 is much more common in squamous lesions (as are all HPVs). The true prevalence of the more recently described types (eg, HPVs 31,33,35,39,42,43,44,45,51,52,56, and others) may be underestimated as they have not been generally available for large-scale screening, but recent epidemiologic studies have helped to clarify worldwide type distributions. 48,49 Thus, well over 95% of premalignant and malignant squamous lesions of the uterine cervix are caused by HPV.…”

Section: Hpv Epithelial Biologymentioning

confidence: 99%

The Impact of Human Papillomavirus Biology on the Clinical Practice of Cervical Pathology

Stoler¹

2005

Pathology Case Reviews

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Human papillomaviruses are present in virtually all cervical cancers and cancer precursors. The transcriptional activity of the virus and the interactions of viral proteins with the epithelial cell determine the pathology of cervical neoplasia. Morphologic criteria are understandable relative to the 2 phenotypes of viral-host interaction (ie, a productive versus a proliferative phenotype). However, the subjective nature of morphologic interpretation has led to an increased utility of HPV and other molecular tests to increase both sensitivity and specificity of cervical cellular diagnosis. The development of HPV vaccines promises to dramatically impact the natural history of HPV infection.H uman papillomaviruses (HPVs) are necessary etiologic agents for cervical neoplasia. More than 2 decades of work has revealed the interplay of these common epitheliotropic viruses with cervical epithelial cells. The pathologic classification of cervical neoplasia, as well as the clinical management of these lesions, has been greatly influenced by these biologic insights. This essay will briefly review these concepts, with an emphasis on the mechanisms by which HPVs produce abnormal cervical morphology, as well as their role in the initiation and promotion of cervical cancer development. These concepts will then be extended to a discussion of the utility of HPV and related tests for diagnosis, as well as a preview of the upcoming event of HPV vaccine implementation.Ancient writings have described skin warts as a problem for humankind. In the beginning of the 20th century, Ciuffo 1 established the viral etiology of human warts (papillomas) by using cell-free extracts from wart tissue as an inoculum for human-to-human transmission experiments. Warts are present in essentially all vertebrate species in which they have been looked for. Shope and Hurst, 2 in 1933, described the first papillomavirus in cottontail rabbits (CRPV). Subsequent experimentation in this system, including the use of coal tar as a tumor promoter, stimulated early concepts of cancer initiation and promotion and confirmed CRPV as the first example of a DNA tumor virus. 2-4 The development of electron microscopy revealed the ultrastructural morphology of papillomaviruses. Clinical studies using electron microscopy revealed that certain kinds of warts were more productive of virions than others. For example, plantar warts often had abundant viral particles, whereas genital warts had relatively few. [5][6][7][8] Papillomaviruses are resistant to tissue culture, making the characterization of these viruses quite difficult. Biochemical and immunologic characterization carried out on viral proteins derived from direct extracts of warts suggested that there was a single type of HPV, an erroneous view that was held through the 1960s. 9 The subsequent revolution in modern biology permitted a more accurate characterization of the papillomavirus family. Clones of the HPV genomes were used to probe different pathologic processes and establish the relationship of those lesion...

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“…We also reported that Southern blot hybridization is more sensitive than immunohistochemistry or electron microscopy (Sato et al 1987), and in situ hybridization is a particularly powerful technique for analyzing the localization of HPV DNA in paraffin-embedded tissues of cervical and vulvar lesions (Tase et al 1988;Sato et al 1989). …”

mentioning

confidence: 92%

Detection of human papillomavirus DNA in genital condylomata in women and their male partners by using in situ hybridization with digoxygenin labeled probes.

Konno

Shikano

Horiguchi

et al. 1990

Tohoku J. Exp. Med.

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Twelve couples (12 women and their male partners) presenting genital warts were investigated in order to evaluate the sexual transmission of human papillomavirus (HPV) in mutual partners and the localization of HPV DNA. Formalin-fixed, paraffin-embedded biopsy samples of 12 vulvar Condylomata, and 12 penile cndylomata from male partners were analyzed for the presence of HPV DNA-6, -11, and 16/18 by using in situ hybridization with digoxygenin labeled DNA probes. HPV DNA was identified in 9 women (75%) and in 9 men (75%). HPV-6 was frequently identified, being revealed in 42% of the vulvar speciemens, in 67% of the cervical specimens and 58% of the penile specimens. Seven of 9 (77%) positive couples shared the same HPV DNA, and 2 couples harbored different HPV DNA types between the partners. The signal intensity of the HPV DNA was generally strong in superficial cell layers, weak in parabasal or basal cell layers. No malignant lesions resulted from the condyloma acuminatum caused by HPV-6 or -11. There were only mild dysplasia in the both sexes.vulva, cervix and penis ; condyloma ; human papillomavirus (HPV) ; male partners ; in situ hybridization Condyloma acuminatum is currently accepted to be a sexually transmitted disease (STD) caused by human papillomavirus (HPV) infection. HPV affects the anogenital tract in both sexes, and evidence has accumulated that HPV infections of the cervix, vulva and penis are associated with epithelial proliferation and malignant lesions (Gissmann et al. 1982;Reid et al. 1982;Durst et al. 1983;Okagaki 1984;Campion et al. 1985;Syrjanen 1986;Schneider et al.

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Sensitivity of Koilocytosis, Immunocytochemistry, and Electron Microscopy as Compared to DNA Hybridization in Detecting Human Papillomavirus in Cervical and Vaginal Condyloma and Intraepithelial Neoplasia

Cited by 36 publications

References 0 publications

Human papillomaviruses: are we ready to type?

Human papillomaviruses: are we ready to type?

The Impact of Human Papillomavirus Biology on the Clinical Practice of Cervical Pathology

Detection of human papillomavirus DNA in genital condylomata in women and their male partners by using in situ hybridization with digoxygenin labeled probes.

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