Sensitivity ofSaccharomyces cerevisiaeto tannic acid is due to iron deprivation

Abstract

Tannic acid inhibited the growth of the yeast Saccharomyces cerevisiae. Growth medium supplementation with more nitrogen or metal ions showed that only iron ions could restore the maximal growth rate of S. cerevisiae. Tannic acid resistant mutants were previously isolated by screening for tannic acid resistance and were all cytoplasmic petite mutants. While the wild type was very sensitive to iron deprivation conditions when grown in aerobic conditions, the mutants, whether grown aerobically or anaerobically, showed the same growth rate under iron-limited conditions as under iron-repleted conditions. Also, the wild type grown anaerobically was not affected by iron-limited conditions. Cytoplasmic petite mutants obtained by ethidium bromide mutagenesis behaved like the other mutants. During iron limitation, the wild type showed a reduced oxygen uptake rate. Maximal growth rate of the wild type in iron-limited conditions could be restored by the addition to the media of unsaturated fatty acids and sterol. Iron deprivation caused by tannic acid may thus affect the synthesis of a functional respiratory chain as well as the synthesis of unsaturated fatty acids and (or) sterol.

A Cytoprotective and Degradable Metal–Polyphenol Nanoshell for Single‐Cell Encapsulation

“…It is noted that the encapsulated cells showed an unperturbed growth rate (m) (0.525 AE 0.032 h À1 for native yeast; 0.594 AE 0.059 h À1 for yeast@[TA-Fe III ] 2 ; 0.569 AE 0.072 h À1 for yeast@[TA-Fe III ] 4 ), even though TA was considered as a restrictive agent for the respiratory chain, possibly leading to slower growth rates and less fermentation products. [17] The result additionally confirmed that the combination of TA and Fe III (and the shell-forming conditions) did not alter the metabolic activities. On the other hand, the HCl treatment (20 mm ; 30 or 90 min) almost restored the division ability of both yeast@[TA-Fe III ] 2 and yeast@[TA-Fe III ] 4 (for 30 min HCl treatment, Figure S6).…”

Respiratory Deficiency Enhances the Sensitivity Candida to Photodynamic Treatment

“…Furthermore, RD mutants of both S. cerevisiae (52,53) and C. glabrata (54) display wall alterations that increased concanavalin A binding to the cell surface. In S. cerevisiae, RD mutants also displayed increased sensitivity to calcoflour white, suggesting a weakened cell wall in these strains (55).…”

Application of Proanthocyanidins from Peanut Skins as a Natural Yeast Inhibitory Agent

“…The PSE could be acting as a yeast inhibitory agent through a variety of mechanisms, either synergistically or not. One of the explanations for yeast inhibition due to tannins (proanthocyanidins) is iron deprivation (Wauters and others 2001). It was found in the Wauters and others (2001) study that growth medium supplementation with more nitrogen or metal ions showed that only iron ions could restore the maximal growth rate of S. cerevisiae .…”

“…One of the explanations for yeast inhibition due to tannins (proanthocyanidins) is iron deprivation (Wauters and others 2001). It was found in the Wauters and others (2001) study that growth medium supplementation with more nitrogen or metal ions showed that only iron ions could restore the maximal growth rate of S. cerevisiae . Tannnins are known transition metal chelators (Rice‐Evans and others 1997; Malesev and Kuntic 2007).…”