Sensitivity of <i>Podosphaera xanthii</i>
 populations to anti-powdery-mildew fungicides in Spain

Bellón-Gómez, Davinia; Vela‐Corcía, David; Pérez-Garcı́a, Alejandro; Torés, J. A.

doi:10.1002/ps.3943

Cited by 33 publications

(21 citation statements)

References 24 publications

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“…On the contrary, spore germination lower than 15% was observed in inoculated leaves treated with the fungicide as positive control, as expected. In fact, this chemical is a well‐known powdery mildew fungicide that prevents powdery mildew sporulation and suppresses new subsequent fungal infections . Most of the metabolites tested significantly reduced powdery mildew spore germination (Table ), with large differences between the effects of the different compounds.…”

Section: Resultsmentioning

confidence: 99%

“…In fact, this chemical is a well-known powdery mildew fungicide that prevents powdery mildew sporulation and suppresses new subsequent fungal infections. 27 Most of the metabolites tested significantly reduced powdery mildew spore germination (Table 2), with large differences between the effects of the different compounds. Metabolites 1, 2 and 3 were effective in reducing spore germination, with an effect that was dose dependent ( Table 2).…”

Section: Inhibition Of Powdery Mildew Spore Germinationmentioning

confidence: 98%

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Impact of fungal and plant metabolites application on early development stages of pea powdery mildew

Barilli

González-Bernal

Cimmino

et al. 2019

Pest Management Science

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BACKGROUND Pea powdery mildew incited by Erysiphe pisi represents a major constraint for pea crops worldwide. Crop protection is largely based on chemical control, although recently a renewed interest in the discovery of natural products as alternatives to synthetic fungicides application has emerged. Thus, 12 bioactive plant and fungal metabolites belonging to different class of natural compounds were evaluated, together with a commercial fungicide, at different concentrations on detached pea leaves for their potential to inhibit spore germination and subsequent stages of fungal growth. The most effective metabolites were tested at different concentrations in planta under controlled conditions to evaluate the level of control achieved by treatments before, concurrently and after pathogen inoculation. Pathogen development was macroscopically scored on whole plants as percentage of disease severity and area under the disease progress curve. RESULTS Cavoxin, inuloxin C and sphaeropsidin A strongly inhibited E. pisi germination and haustoria formation and reduced colony size. This effect was dose dependent. These results were further confirmed in whole plants by spraying the metabolites on plant leaves for preventive or curative control, which reduced fungal developmental of E. pisi at levels comparable with those obtained by application of the fungicide. CONCLUSIONS Cavoxin, inuloxin C and sphaeropsidin A have potential as alternatives to synthetic fungicides for the control of crop pathogens of economic importance such as powdery mildew. © 2019 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Inhibition Of Powdery Mildew Spore Germinationmentioning

confidence: 98%

Impact of fungal and plant metabolites application on early development stages of pea powdery mildew

Barilli

González-Bernal

Cimmino

et al. 2019

Pest Management Science

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“…However, mutations in SQR subunits have conferred SDHI resistance in 14 fungal species thus far 24 , highlighting the need for new antifungal agents with diverse mechanisms of action. Although F. graminearum remains susceptible to chemical SDHIs for the time being, mutations that reduce susceptibility have been identified in a number of pathogens including B. cinerea 32 , 33 , Podosphaera xanthii 34 , 35 , Alternaria spp. 36 , 37 and Sclerotinia sclerotiorum 38 .…”

Section: Discussionmentioning

confidence: 99%

The selective antifungal activity of Drosophila melanogaster metchnikowin reflects the species-dependent inhibition of succinate–coenzyme Q reductase

Moghaddam

Gross

Becker

et al. 2017

Sci Rep

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Insect-derived antifungal peptides have a significant economic potential, particularly for the engineering of pathogen-resistant crops. However, the nonspecific antifungal activity of such peptides could result in detrimental effects against beneficial fungi, whose interactions with plants promote growth or increase resistance against biotic and abiotic stress. The antifungal peptide metchnikowin (Mtk) from Drosophila melanogaster acts selectively against pathogenic Ascomycota, including Fusarium graminearum, without affecting Basidiomycota such as the beneficial symbiont Piriformospora indica. Here we investigated the mechanism responsible for the selective antifungal activity of Mtk by using the peptide to probe a yeast two-hybrid library of F. graminearum cDNAs. We found that Mtk specifically targets the iron-sulfur subunit (SdhB) of succinate–coenzyme Q reductase (SQR). A functional assay based on the succinate dehydrogenase (SDH) activity of mitochondrial complex II clearly demonstrated that Mtk inhibited the SDH activity of F. graminearum mitochondrial SQR by up to 52%, but that the equivalent enzyme in P. indica was unaffected. A phylogenetic analysis of the SdhB family revealed a significant divergence between the Ascomycota and Basidiomycota. SQR is one of the key targets of antifungal agents and we therefore propose Mtk as an environmentally sustainable and more selective alternative to chemical fungicides.

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“…10-mm discs were excised with a sterilized cork borer, incubated with cell-free supernatants for 2 h, and then left to dry. Finally, the discs were inoculated with P. xanthii conidia as previously described 99 .…”

Section: Methodsmentioning

confidence: 99%

Dual functionality of the TasA amyloid protein inBacillusphysiology and fitness on the phylloplane

Cámara-Almirón

Navarro

Magno-Pérez-Bryan

et al. 2019

Preprint

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AbstractBacteria can form biofilms that consist of multicellular communities embedded in an extracellular matrix (ECM). Previous studies have demonstrated that genetic pathways involved in biofilm formation are activated under a variety of environmental conditions to enhance bacterial fitness; however, the functions of the individual ECM components are still poorly understood. In Bacillus subtilis, the main protein component of the ECM is the functional amyloid TasA. In this study, we demonstrate that beyond their well-known defect in biofilm formation, ΔtasA cells also exhibit a range of cytological symptoms indicative of excessive cellular stress, including DNA damage accumulation, changes in membrane potential, higher susceptibility to oxidative stress, and alterations in membrane dynamics. Collectively, these events can lead to increased programmed cell death in the colony. We show that these major physiological changes in ΔtasA cells are likely independent of the structural role of TasA during amyloid fiber formation in the ECM. The presence of TasA in cellular membranes, which would place it in proximity to functional membrane microdomains, and mislocalization of the flotillin-like protein FloT in ΔtasA cells, led us to propose a role for TasA in the stabilization of membrane dynamics as cells enter stationary phase. We found that these alterations caused by the absence of TasA impair the survival, colonization and competition of Bacillus cells on the phylloplane. Taken together, our results allow the separation of two complementary roles of this functional amyloid protein: i) structural functions during ECM assembly and interactions with plants, and ii) a physiological function in which TasA, via its localization to the cell membrane, stabilizes membrane dynamics and supports more effective cellular adaptation to environmental cues.

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Sensitivity of Podosphaera xanthii populations to anti-powdery-mildew fungicides in Spain

Abstract: Results suggest that thiophanate-methyl has become completely ineffective for controlling cucurbit powdery mildew in Spain. By contrast, bupirimate and quinoxyfen remain as very effective tools for cucurbit powdery mildew management. © 2014 Society of Chemical Industry.

Cited by 33 publications

References 24 publications

Impact of fungal and plant metabolites application on early development stages of pea powdery mildew

Impact of fungal and plant metabolites application on early development stages of pea powdery mildew

The selective antifungal activity of Drosophila melanogaster metchnikowin reflects the species-dependent inhibition of succinate–coenzyme Q reductase

Dual functionality of the TasA amyloid protein inBacillusphysiology and fitness on the phylloplane

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