Sensitivity of hydrogen bonds of DNA and RNA to hydration, as gauged by 1 J NH measurements in ethanol–water mixtures

Manalo, Marlon N.; Kong, Xiangzhen; LiWang, Andy

doi:10.1007/s10858-006-9132-8

Cited by 7 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chemical shifts of these amide protons of thymine and uracil in the complexes of T‐C16/A‐C16 and U‐C16/T‐C16 were used to obtain values of K a of 534 and 671 M –1 from Benesi–Hildebrand plots, respectively (see Supporting Information). The 1 H NMR titration experiments reveal that the hydrogen bonding strength of the U‐A base pair of RNA is stronger than that of T‐A base pair of DNA as previously described,3, 8, 9 indirect representing the relatively stronger strength of hydrogen bonding interaction within PVBU/PVBT complex.…”

Section: Resultssupporting

confidence: 61%

See 1 more Smart Citation

Never‐smoking nonsmall cell lung cancer as a separate entity

Yano

Miura

Takenaka

et al. 2008

Cancer

119

104

View full text Add to dashboard Cite

BACKGROUND.To propose ‘never‐smoking nonsmall cell lung cancer (NSCLC)’ as a separate entity, the clinicopathologic differences of operable NSCLC between never‐smoking patients and patients with a history of smoking were investigated.METHODS.The medical records of 1405 patients with primary NSCLC who underwent a complete resection at the study institution from 1974 through 2004 were reviewed for clinicopathologic variables and postoperative survival.RESULTS.The proportion of never‐smoking patients with NSCLC has been significantly increasing over 30 years, from 15.9% in the 1970s to 32.8% in the 2000s. A significantly greater proportion of female patients or adenocarcinoma patients was found in the ‘never‐smoking NSCLC’ group in comparison to the ‘smoking NSCLC’ group (85.8% vs 11.2% and 87.8% vs 49.1%, respectively). The proportion of pathologic stage IA disease for the ‘never‐smoking NSCLC’ group was significantly higher than that for the ‘smoking NSCLC’ group (40.1% vs 25.4%; P < .0001). With regard to both overall and cancer‐specific survival, the ‘never‐smoking NSCLC’ patient group was significantly superior to the ‘smoking NSCLC’ group. In addition to smoking status, the factors found to be significantly associated with the postoperative survival rate were sex, histologic type, T classification, and N classification by univariate analyses. A multivariate analysis revealed never‐smoking status to be an independent prognostic factor in addition to pathologic T and N classification.CONCLUSIONS.The differences in the clinicopathologic factors and survivals between the ‘never‐smoking NSCLC’ patient group and the ‘smoking NSCLC’ group suggest that NSCLC in never‐smokers should be considered a separate disease entity. Cancer 2008. © 2008 American Cancer Society.

show abstract

Section: Resultssupporting

confidence: 61%

“…Only a limited number of reports have appeared so far to compare the hydrogen bonding interactions between DNA (T‐A) and RNA (U‐A) base pairs 1–7. Recently, Wang and coworkers8, 9 compared 2h Δ 13 C2 DNA and 2h Δ 13 C2 RNA and indicated that the RNA (U‐A) hydrogen bond is about 3% stronger than the DNA (A‐T) hydrogen bond.…”

Section: Introductionmentioning

confidence: 99%

Never‐smoking nonsmall cell lung cancer as a separate entity

Yano

Miura

Takenaka

et al. 2008

Cancer

119

104

View full text Add to dashboard Cite

show abstract

“…The chemical shifts of these amide protons of thymine and uracil in the complexes of T-C16/A-C16 and U-C16/T-C16 were used to obtain values of K a of 534 and 671 M -1 from Benesi-Hildebrand plots, respectively (see Supporting Information). The 1 H NMR titration experiments reveal that the hydrogen bonding strength of the U-A base pair of RNA is stronger than that of T-A base pair of DNA as previously described, 3,8,9 indirect representing the relatively stronger strength of hydrogen bonding interaction within PVBU/ PVBT complex. Figure 3 displays DSC traces of PVBT/PVBA and PVBU/PVBA complexes, indicating that the glass transition temperatures (T g ) of pure PVBT and PVBU are about 188 and 141 C, respectively.…”

Section: Comparison Of Hydrogen Bonds Between the T-a And U-a Base Pairssupporting

confidence: 62%

Biocomplementary interaction behavior in DNA‐like and RNA‐like polymers

Cheng

Yen

et al. 2009

J. Polym. Sci. A Polym. Chem.

View full text Add to dashboard Cite

A series of nucleobased polymers and copolymers were synthesized through atom transfer radical polymerization (ATRP). Biocomplementary DNA‐ and RNA‐like supramolecular complexes are formed in dilute DMSO solution through nucleobase recognition. 1H NMR titration studies of these complexes in CDCl3 indicated that thymine‐adenine (T‐A) and uracil‐adenine (U‐A) complexes form rapidly on the NMR time scale with high association constants (up to 534 and 671 M–1, respectively) and result in significant Tg increase. WAXD and differential scanning calorimetry analyzes in the bulk state indicate the presence of highly physical cross‐linked structures and provide further details into the nature of the self‐assembly of these systems. Furthermore, this study is of discussion on the difference in the hydrogen bond strength between T‐A and U‐A base pairs within polymer systems, indicating that the strength of hydrogen bonds in RNA U‐A pairs is stronger than that in DNA T‐A base pairs. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6388–6395, 2009

show abstract

“…In order to determine if either or both of these characteristics are present, we examined the impact of temperature on 1 J NH . The magnitude of 1 J NH has previously been used as an effective tool for detecting hydrogen bonds in biomolecules,47, 48 mainly because 1 J NH is not affected by changes in ϕ/ψ torsion angles. Analogous to the effect of temperature change on averaged NH chemical shifts, as described above, increase in sample temperature also influences average 1 J NH values due to an increase in the population in low‐lying unfolded states.…”

Section: Resultsmentioning

confidence: 99%

Transient hydrogen bonding in uniformly ¹³C,¹⁵N‐Labeled Carbohydrates in Water

Norris¹,

Landström²,

Weintraub³

et al. 2011

Biopolymers

View full text Add to dashboard Cite

We report NMR studies of transient hydrogen bonding in a polysaccharide (PS) dissolved in water without cosolvent at ambient temperature. The PS portion of the Escherichia coli O142 lipopolysaccharide is comprised of repeating pentasaccharide units of GalNAc (N-acetyl galactosamine), GlcNAc (N-acetyl glucosamine), and rhamnose in a 3:1:1 ratio, respectively. A 105-ns molecular dynamics (MD) simulation on one pentasaccharide repeat unit predicts transient inter-residue hydrogen bonds from the GalNAc NH groups in the PS. To investigate these predictions experimentally, the PS was uniformly ¹³C,¹⁵N enriched and the NH, carbonyl, C2, C4, and methyl resonances of the GalNAc and GlcNAc residues assigned using through-bond triple-resonance NMR experiments. Temperature dependence of amide NH chemical shifts and one-bond NH J couplings support that NH groups on two of the GalNAc residues are donors in transient hydrogen bonds. The remaining GalNAc and GlcNAc NHs do not appear to be donors from either temperature-dependent chemical shifts or one-bond NH J couplings. These results substantiate the presence of weak or partial hydrogen bonds in carbohydrates, and that MD simulations of repeating units in PSs provide insight into overall PS structure and dynamics.

show abstract

Sensitivity of hydrogen bonds of DNA and RNA to hydration, as gauged by 1 J NH measurements in ethanol–water mixtures

Cited by 7 publications

References 39 publications

Never‐smoking nonsmall cell lung cancer as a separate entity

Never‐smoking nonsmall cell lung cancer as a separate entity

Biocomplementary interaction behavior in DNA‐like and RNA‐like polymers

Transient hydrogen bonding in uniformly ¹³C,¹⁵N‐Labeled Carbohydrates in Water

Contact Info

Product

Resources

About

Sensitivity of hydrogen bonds of DNA and RNA to hydration, as gauged by 1 J NH measurements in ethanol–water mixtures

Cited by 7 publications

References 39 publications

Never‐smoking nonsmall cell lung cancer as a separate entity

Never‐smoking nonsmall cell lung cancer as a separate entity

Biocomplementary interaction behavior in DNA‐like and RNA‐like polymers

Transient hydrogen bonding in uniformly 13C,15N‐Labeled Carbohydrates in Water

Contact Info

Product

Resources

About

Transient hydrogen bonding in uniformly ¹³C,¹⁵N‐Labeled Carbohydrates in Water