Sensitivity of coded aperture Raman spectroscopy to analytes beneath turbid biological tissue and tissue-simulating phantoms

Maher, Jason R.; Matthews, Thomas E.; Reid, Ashley K.; Katz, David F.; Wax, Adam

doi:10.1117/1.jbo.19.11.117001

Cited by 10 publications

(5 citation statements)

References 41 publications

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“…Measurements were taken of snoutto-vent length (SVL), head width (HW), body mass and tail length for all tadpoles; liver weights were also taken for tadpoles collected from CT and WH in 2013. After fixation, the following organs were excised: liver, kidney, heart, muscle, and skin, and slices (~0.5 mm thick) taken using a Stadie-Riggs tissue slicer; a technique previously employed for preparing tissue samples for spectroscopy studies (Maher et al, 2014;Obinaju et al, 2014;Taylor et al, 2011). Slices of each organ were mounted onto Low-E reflective glass slides (Kevley Technologies, Chesterland, OH, USA), dried overnight and stored in a desiccator before subsequent interrogation with ATR-FTIR spectroscopy.…”

Section: Tadpole Collectionmentioning

confidence: 99%

Biospectroscopy reveals the effect of varying water quality on tadpole tissues of the common frog (Rana temporaria)

Strong

Halsall

Ferenčík

et al. 2016

Environmental Pollution

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Amphibians are undergoing large population declines in many regions around the world. As environmental pollution from both agricultural and urban sources has been implicated in such declines, there is a need for a biomonitoring approach to study potential impacts on this vulnerable class of organism. This study assessed the use of infrared (IR) spectroscopy as a tool to detect changes in several tissues (liver, muscle, kidney, heart and skin) of late-stage common frog (Rana temporaria) tadpoles collected from ponds with differing water quality. Small differences in spectral signatures were revealed between a rural agricultural pond and an urban pond receiving wastewater and landfill run-off; these were limited to the liver and heart, although large differences in body size were apparent, surprisingly with tadpoles from the urban site larger than those from the rural site. Large differences in liver spectra were found between tadpoles from the pesticide and nutrient impacted pond compared to the rural agricultural pond, particularly in regions associated with lipids. Liver mass and hepatosomatic indices were found to be significantly increased in tadpoles from the site impacted by pesticides and trace organic chemicals, suggestive of exposure to environmental contamination. Significant alterations were also found in muscle tissue between tadpoles from these two ponds in regions associated with glycogen, potentially indicative of a stress response. This study highlights the use of IR spectroscopy, a low-cost, rapid and reagent-free technique in the biomonitoring of a class of organisms susceptible to environmental degradation.

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Section: Tadpole Collectionmentioning

confidence: 99%

Biospectroscopy reveals the effect of varying water quality on tadpole tissues of the common frog (Rana temporaria)

Strong

Halsall

Ferenčík

et al. 2016

Environmental Pollution

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“…For ATR-FTIR spectroscopy measurements, a longitudinal slice (~0.5 mm thick) was taken from the ventral side of the tadpole using a Stadie-Riggs tissue slicer; a simple technique previously employed for preparing tissue samples for analysis with IR spectroscopy 31 63 64 . Slices were mounted skin side down onto Low-E slides, dried overnight and stored in a desiccator before interrogation with ATR-FTIR spectroscopy.…”

Section: Methodsmentioning

confidence: 99%

Subtle effects of environmental stress observed in the early life stages of the Common frog, Rana temporaria

Strong

Martin

Jones

et al. 2017

Sci Rep

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Worldwide amphibian populations are declining due to habitat loss, disease and pollution. Vulnerability to environmental contaminants such as pesticides will be dependent on the species, the sensitivity of the ontogenic life stage and hence the timing of exposure and the exposure pathway. Herein we investigated the biochemical tissue ‘fingerprint’ in spawn and early-stage tadpoles of the Common frog, Rana temporaria, using attenuated total reflection-Fourier-transform infrared (ATR-FTIR) spectroscopy with the objective of observing differences in the biochemical constituents of the respective amphibian tissues due to varying water quality in urban and agricultural ponds. Our results demonstrate that levels of stress (marked by biochemical constituents such as glycogen that are involved in compensatory metabolic mechanisms) can be observed in tadpoles present in the pond most impacted by pollution (nutrients and pesticides), but large annual variability masked any inter-site differences in the frog spawn. ATR-FTIR spectroscopy is capable of detecting differences in tadpoles that are present in selected ponds with different levels of environmental perturbation and thus serves as a rapid and cost effective tool in assessing stress-related effects of pollution in a vulnerable class of organism.

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“…When measuring bone, confocal RS is primarily sensitive to the cortex, as the transport length of near‐infrared light in bone is only about 500 microns . Diffuse Raman measurements, such as those acquired with coded aperture spectrometers , can sense significantly deeper within the tissue, but provide much coarser resolution that may preclude the separation of signals from different bone layers. Spatially offset Raman spectroscopy (SORS) is another approach that was specifically developed to measure turbid, layered media .…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of spatially offset Raman spectroscopy (SORS) to subcortical bone tissue

et al. 2017

Self Cite

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The development of spatially offset Raman spectroscopy (SORS) has enabled deep, non-invasive chemical characterization of turbid media. Here, we use SORS to measure subcortical bone tissue and depth-resolved biochemical variability in intact, exposed murine bones. We also apply the technique to study a mouse model of the genetic bone disorder osteogenesis imperfecta. The results suggest that SORS is more sensitive to disease-related biochemical differences in subcortical trabecular bone and marrow than conventional Raman measurements.

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Sensitivity of coded aperture Raman spectroscopy to analytes beneath turbid biological tissue and tissue-simulating phantoms

Cited by 10 publications

References 41 publications

Biospectroscopy reveals the effect of varying water quality on tadpole tissues of the common frog (Rana temporaria)

Biospectroscopy reveals the effect of varying water quality on tadpole tissues of the common frog (Rana temporaria)

Subtle effects of environmental stress observed in the early life stages of the Common frog, Rana temporaria

Sensitivity of spatially offset Raman spectroscopy (SORS) to subcortical bone tissue

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