1999
DOI: 10.1093/hmg/8.9.1715
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Sensitivity Issues in DNA Array-Based Expression Measurements and Performance of Nylon Microarrays for Small Samples

Abstract: DNA or oligonucleotide arrays are widely used for large-scale expression measurements, using various implementations: macroarrays in which DNA is spotted onto nylon membranes of relatively large dimensions (with radioactive detection) on the one hand; microarrays on glass slides and oligonucleotide chips, both used with fluorescent probes, on the other hand. Nylon micro-arrays with colourimetric detection have also been described recently. The small physical dimensions of miniaturized systems allow small hybri… Show more

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Cited by 169 publications
(112 citation statements)
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“…Data were further corrected for the amount of spotted cDNA (Bertucci et al, 1999). From the initial set of 7.824 genes related to carcinogenesis, 4.716 were significantly expressed in a majority of samples and were kept for further analysis.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Data were further corrected for the amount of spotted cDNA (Bertucci et al, 1999). From the initial set of 7.824 genes related to carcinogenesis, 4.716 were significantly expressed in a majority of samples and were kept for further analysis.…”
Section: Discussionmentioning
confidence: 99%
“…Several quality control steps were taken. Before each RNA hybridization, the quality of spotting, including the determination of target DNA amount accessible for each spot, was controlled by hybridization of each microarray with a 33 P-labeled oligonucleotide sequence common to all PCR products (Bertucci et al, 1999). After stripping, microarrays were hybridized with 33 P-labeled probes made from total RNA.…”
Section: Microarray Proceduresmentioning
confidence: 99%
“…A-MEXP-50 and E-MEXP-393; ͗www.ebi.ac.uk/miamexpress͘). The feasibility, reproducibility, and sensitivity of spotting procedures have been previously described (25)(26)(27). Nylon microarrays were prepared as described using 3277 IMAGE cDNA clones (3158 nonredundant) coding for genes involved in the immunological process and ubiquitous biological function (26).…”
Section: Microarraymentioning
confidence: 99%
“…Nylon filter preparation with spotted polymerase chain reaction (PCR) products derived from ϳ1000 selected candidate cancer genes, 33 P radioactive hybridization, and data acquisition, normalization, and analysis have been described elsewhere 13,14 and can also be consulted on our web site (http:/tagc.univ-mrs.fr/pub/Cancer/). …”
Section: Dna Arraysmentioning
confidence: 99%