Sensitivity Enhancement of Optical Immunosensors with Nanoparticles

Kubitschko, S.; Spinke, J.; Brückner, Timon; Pohl, Stefan; Oranth, Norbert

doi:10.1006/abio.1997.2337

Cited by 128 publications

(80 citation statements)

References 14 publications

(3 reference statements)

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“…A number of researchers have used polystyrene beads bound to the target antigens to enhance the sensitivity of refractometric measurements [7]. This is particularly useful when the target is a small molecule such as a drug.…”

Section: Sensitivitymentioning

confidence: 99%

Optical Properties of Bound Antigen Monolayers for Biomolecular Microsensors

Cohen¹

2004

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REPORT DOCUMENTATION PAGEForm Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. REPORT TYPE AND DATES COVEREDFinal Aug 01 -Sep 03 TITLE AND SUBTITLE OPTICAL PROPERTIES OF BOUND ANTIGEN MONOLAYERS FOR BIOMOLECULAR MICROSENSORS AUTHOR(S)Daniel A. Cohen FUNDING NUMBERSC -F30602-01-2-0538 PE -61101E PR -E117 TA -00 WU -67 PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)University 12b. DISTRIBUTION CODE ABSTRACT (Maximum 200 Words)The formalism of molar refraction was extended to cover the wavelength range of 200 nm -2500 nm, in order to predict the index of refraction and index increment of a wide range of molecules of biochemical interest. Data for the model was obtained using a unique interferometer cell within a standard laboratory spectrophotometer. The resulting models for protein index increment suggest that both the sensitivity and specificity of refractometric sensors may be improved by operating at ultraviolet wavelengths and that measurement at two wavelengths may be used to quantify the total protein present and distinguish between similar proteins, of potential use to reduce false positive responses in affinity assays. The impact of refractive index and particle size on semiconductor-based evanescent field sensors was analyzed, along with the magnitude of optical scattering. The results are particularly important in quantitatively explaining the benefits of using a nanoparticle-enhanced assay, and of using high-index nanoparticles for enhancement. For particles below 100 nm in diameter, optical scattering will be negligible. For particles larger than 100 nm, scattering becomes measurable, and single one-micron diameter particles were detected in a novel monolithic evanescent field sensor. NUMBER OF PAGES 26

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Section: Sensitivitymentioning

confidence: 99%

Optical Properties of Bound Antigen Monolayers for Biomolecular Microsensors

Cohen¹

2004

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“…For example, the detection limit of steroid hormone was thus reduced from 12 to 7ng·ml -1 (Kubitschko et al 1997). The mass-amplified assay is operated in two stages.…”

Section: Introductionmentioning

confidence: 99%

Capabilities of Piezoelectric Immunosensors for Detecting Infections and for Early Clinical Diagnostics

Ermolaeva¹,

Kalmykova²

2012

Advances in Immunoassay Technology

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“…BIAcore SPR instruments have been widely used for years for the analysis of biomolecular interactions. It is extensively applied as a research tool to examine antibody / antigen (McCormick et al, 2004;Kubitschko et al, 1997), oligonucleotide / complementary oligonucleotide (Kai et al, 1999), receptor / target (Zhang et al, 2004) and host / guest (Chen et al, 2002) interactions. These instruments contain sophisticated microfluidics and temperature control units and range from the conventional four channel versions such as BIAcore 3000, to the more recent array format in the BIAcore Flexchip.…”

Section: Introductionmentioning

confidence: 99%