Sensitivity enhancement of a “bananatrode” biosensor for dopamine based on SECM studies inside its reaction layer

Őri, Zsuzsanna; Ciucu, Anton Alexandru; Mihailciuc, Constantin; Stefanescu, Cristian Dragos; Nagy, Lı́via; Nagy, Géza

doi:10.1016/j.snb.2013.08.063

Cited by 6 publications

(2 citation statements)

References 30 publications

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“…5b). When the graph was examined, it was determined that the highest reduction current of dopamine-o-quinone was at −0.15 V. In the literature, it was stated that DA biosensors studied at different potentials as −0.1 V, 19 −0.15 V 12 and −0.2 V. 20…”

Section: Resultsmentioning

confidence: 99%

A Sensitive Amperometric Biosensor Based on Carbon Dot 3-Chloropropyl-trimethoxysilane Modified Electrode for Detection of Neurotransmitter Dopamine

Akbıyık

Bodur²,

Keskin³

et al. 2023

J. Electrochem. Soc.

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Dopamine (DA) is an important electroactive neurotransmitter. The concentration of DA in the body of a healthy person is approximately 1.0x10-7-1.0x10-3 M. A decrease in dopamine concentration is associated with Parkinson’s disease. Thus, it is important to determine the amount of dopamine in early diagnosis of Parkinson’s disease. Different methods such as immunoassay, flow injection analysis (FIA), high performance liquid chromatography (HPLC) etc. could be used for determination of dopamine but they are expensive and have long determination times, and pre analytic processes. In this study, a tyrosinase based amperometric biosensor was developed with carbon paste electrode modified with carbon nano dot 3-Chloropropyl-trimethoxysilane (CDs-CPTMS) for determination of the amount of dopamine. CDs-CPTMS was synthesized for the first time. Determination of dopamine was carried out by the reduction of dopamine-o-quinone at -0.15 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the dopamine response of the prepared biosensor and interference effect were investigated. There was no interference effect of uric acid and ascorbic acid. The designed biosensor has wide working range (0.001- 0.01μM and 0.01- 0.1 μM), low limit of detection, very good reproducibility and shelf life. In addition, the preparation of the biosensor is practical and cost-effective.

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Section: Resultsmentioning

confidence: 99%

A Sensitive Amperometric Biosensor Based on Carbon Dot 3-Chloropropyl-trimethoxysilane Modified Electrode for Detection of Neurotransmitter Dopamine

Akbıyık

Bodur²,

Keskin³

et al. 2023

J. Electrochem. Soc.

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“…In the construction of tissue-based electrochemical biosensors, tissue homogenates from various living organisms such as pigs (Thoppe Rajendran et al 2020), mushrooms (Sezgintürk & Dinçkaya 2012) and plant tissues including banana (Ozcan & Sagiroglu 2010), artichoke (Odaci et al 2004), Myrtle (Ayna & Akyilmaz 2018) are employed for detection of toxins (Sanders et al 2001), drugs (Thoppe Rajendran et al 2020), herbicides (Breton et al 2006) and phenolic compounds such as rutin (Zwirtes de Oliveira et al 2006), epinephrine (Felix et al 2006), caffeic acid (Fernandes et al 2007), catechol (Ozcan & Sagiroglu 2010) and dopamine (Ori et al 2014).…”

Section: Introductionmentioning

confidence: 99%

SENSITIVE DETERMINATION OF 3,4-DIHYDROXY-L-PHENYLALANINE BY A CLOUD FUNNEL MUSHROOM (Clitocybe nebularis (Batsch), P. Kumm.) HOMOGENATE-BASED AMPEROMETRIC BIOSENSOR

Asav

2021

Trakya University Journal of Natural Sciences

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3,4-dihydroxy-L-phenylalanine (L-DOPA) is one of the precursor molecules for the biosynthesis of neurotransmitters in the brain. Monitoring of L-DOPA levels as a drug or biomolecule in biological fluids is crucial for the treatment of patients suffering from Parkinson's Disease. This study aimed to construct a cloud funnel mushroom (Clitocybe nebularis (Batsch), P. Kumm.) tissue homogenate-based biosensor for precise and sensitive detection of L-DOPA in artificial plasma and urine. For this purpose, in the fabrication of the biosensor, tissue homogenate of C. nebularis was immobilized into a carbon paste electrode by using graphite, mineral oil, gelatine and glutaraldehyde. The amperometric signals corresponding to 600 s were recorded as response current for each L-DOPA concentration. All amperometric measurements were carried out at ⁻700 mV (versus Ag|AgCl). The present biosensor successfully detected L-DOPA with a linear dynamic range at 2.5-100 µM and Limit of Detection (LOD) value as 0.76 µM, as well as standard deviation as ±0.41 µM and coefficient of variation as 0.82% (n=16). Additionally, the determination of L-DOPA spiked in artificial plasma and urine was carried out successfully. The present work would be the first study that utilized C. nebularis tissue as a biosensor component.Özet: 3,4-dihidroksifenilalanin (L-DOPA), beyinde nörotransmitter sentezi için öncül moleküllerden biridir. Biyolojik sıvılarda, ilaç veya biyomolekül olarak L-DOPA düzeylerinin izlenmesi, Parkinson hastalığına sahip kişilerin tedavi süreci için önemlidir. Bu çalışmanın amacı, sentetik plazma ve idrar örneklerinde L-DOPA molekülünün doğru ve duyarlı bir tayinine yönelik, bulutlu huni mantarı (Clitocybe nebularis (Batsch), P. Kumm.) doku homejenatı temelli bir biyosensör sistemi geliştirmektir. Bu bağlamda, biyosensör yapımında, C. nebularis doku homojenatı; grafit, mineral yağ, jelatin ve glutaraldehit kullanılarak karbon pasta elektrot içine immobilize edilmişlerdir. 600. saniyeye karşılık gelen amperometrik sinyaller her L-DOPA konsantrasyonu için yanıt akımı olarak kaydedilmiştir. Tüm amperometrik ölçümler ⁻700 mV (vs Ag|AgCl) potansiyelinde gerçekleştirilmiştir. Geliştirilen biyosensör, L-DOPA molekülünü 2,5-100 µM tayin aralığında ve 0,76 µM tayin limitinin yanı sıra ±0,41 µM standart sapma (n= 16). ve %0,82 varyasyon katsayısı ile saptayabilmiştir. Ayrıca sentetik plazma ve idrar içerisine eklenmiş L-DOPA miktarının da tayini başarı ile gerçekleştirilmiştir. Bu çalışma C. nebularis dokusunun ilk kez bir biyosensör bileşeni olarak kullanıldığı çalışmadır.Protein assays via BSA and Coomassie Brilliant Blue G-250 were carried out according to the Standard Bradford method (Bradford 1976) for determination of the protein amounts of C. nebularis tissue homogenates.A modified version of the ABTS method described by Shin & Lee (2000) was used for determination of laccase activity of tissue homogenates. The absorbance values at 420 nm and 25°C of the assay mixture containing tissue homogenates (0.1 mL) and ABTS (0.9 mL) were

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Graphene Paste Electrode: Analytical Applications for the Quantification of Dopamine, Phenolic Compounds and Ethanol

et al. 2014

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This work reports the analytical applications of a graphene paste electrode (GrPE) for the quantification of dopamine, ethanol and phenolic compounds. Dopamine was detected by differential pulse voltammetry‐adsorptive stripping with medium exchange at submicromolar levels even in the presence of high excess of ascorbic acid and serotonin. The electrocatalytic activity of graphene towards the oxidation of NADH and the reduction of quinones allowed the sensitive amperometric determination of ethanol and phenols using GrPE modified with alcohol dehydrogenase/NAD+ or polyphenol oxidase, respectively, with successful applications in real samples like alcoholic beverages and tea.

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Sensitivity enhancement of a “bananatrode” biosensor for dopamine based on SECM studies inside its reaction layer

Cited by 6 publications

References 30 publications

A Sensitive Amperometric Biosensor Based on Carbon Dot 3-Chloropropyl-trimethoxysilane Modified Electrode for Detection of Neurotransmitter Dopamine

A Sensitive Amperometric Biosensor Based on Carbon Dot 3-Chloropropyl-trimethoxysilane Modified Electrode for Detection of Neurotransmitter Dopamine

SENSITIVE DETERMINATION OF 3,4-DIHYDROXY-L-PHENYLALANINE BY A CLOUD FUNNEL MUSHROOM (Clitocybe nebularis (Batsch), P. Kumm.) HOMOGENATE-BASED AMPEROMETRIC BIOSENSOR

Graphene Paste Electrode: Analytical Applications for the Quantification of Dopamine, Phenolic Compounds and Ethanol

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